Blasts from the past: is morphology useful in PGT-A tested and untested frozen embryo transfers?

Shear, Matthew A.; Vaughan, Denis A.; Modest, Anna M.; Seidler, Emily A.; Leung, Angela Q.; Hacker, Michele R.; Sakkas, Denny; Penzias, Alan S.

doi:10.1016/j.rbmo.2020.07.014

Cited by 25 publications

(6 citation statements)

References 42 publications

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“…These results align with those of previous reports that used PGT-A, with similar outcomes for day-5 or day-6 euploid transfers (22)(23)(24)(25). A recent study evaluating the effect of the day of cryopreservation showed that in frozen transfers of euploid embryos assessed by PGT-A, the day of cryopreservation did not significantly affect live birth rates (26).…”

Section: What Additional Evidence Is Needed To Support the Pro?supporting

confidence: 90%

Noninvasive preimplantation genetic testing for aneuploidy in spent culture medium as a substitute for trophectoderm biopsy

Rubio

Racowsky

Barad

et al. 2021

Fertility and Sterility

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Section: What Additional Evidence Is Needed To Support the Pro?supporting

confidence: 90%

Noninvasive preimplantation genetic testing for aneuploidy in spent culture medium as a substitute for trophectoderm biopsy

Rubio

Racowsky

Barad

et al. 2021

Fertility and Sterility

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“…In laboratories, morphological evaluation has been the main strategy applied to choose the embryos to transfer. Most reports demonstrate that the implantation, clinical pregnancy and live birth rates in the day-5 blastocyst group are significantly higher than those in the day-6 blastocyst group[ 12 , 26 , 34 ] and that morphologic parameters (degree of expansion, ICM grade, and TE grade) are significantly positively correlated with the outcomes after blastocyst transfer[ 27 , 29 ]. Some studies suggested that the transfer of euploid blastocysts tested by PGT-A can lead to higher rates of clinical pregnancy, live birth, and implantation than those of non-PGT-A tested blastocysts [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

The correlation between morphological parameters and the incidence of de novo chromosomal abnormalities in 3238 biopsied blastocysts

Gao

Wei

Zhu

et al. 2023

J Assist Reprod Genet

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Purpose The aim of this study was to determine the relationship between morphological parameters and the incidence of de novo chromosomal abnormalities. Methods This was a retrospective cohort study of 652 patients who underwent 921 cycles with 3238 blastocysts biopsied. The embryo grades were evaluated according to Gardner and Schoolcraft’s system. The incidence of euploidy, whole chromosomal aneuploidy (W-aneuploidy), segmental chromosomal aneuploidy (S-aneuploidy), and mosaicism in trophectoderm (TE) cell biopsies was analyzed. Results The euploidy decreased significantly with maternal age and was positively correlated biopsy day and morphological parameters. The W-aneuploidy increased significantly with maternal age and was negatively correlated biopsy day and morphological parameters. Parental age, TE biopsy day, and morphological parameters were not associated with S-aneuploidy and mosaicism, except that TE grade C blastocysts had significantly higher mosaicism than TE grade A blastocysts. Subanalysis in different female age groups showed that euploidy and W-aneuploidy had a significant correlation with TE biopsy day among women aged ≤ 30 y and 31–35 y, with expansion degree among women aged ≥ 36 y, with ICM grade among women aged ≥ 31 y, and with TE grade among all female age ranges. Conclusion Female age, embryo developmental speed and blastocyst morphological parameters are associated with euploidy and whole chromosomal aneuploidy. The predictive value of these factors varies across female age groups. Parental age, embryo developmental speed, expansion degree, and ICM grade are not associated with the incidence of segmental aneuploidy or mosaicism, but TE grade seemingly has a weak correlation with segmental aneuploidy and mosaicism in embryos.

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“…The main concern with extended embryo culture is the impact on viability and reproductive potential if embryos are left in culture to day 6. Notably, several studies showed that in PGT-A cases with day-6 vitrification, clinical outcomes were similar to those with day-5 embryos (Kaye et al, 2017;Tiegs et al, 2019;Viñals-Gonzalez et al, 2019;Shear et al, 2020). Additionally, in a pilot study using niPGT-A and culturing blastocysts until day 6, pregnancy rates were high and similar to PGT-A cases with day-5 or -6 vitrification (Franco et al, 2021).…”

Section: ) How Long Should the Medium Be In Contact With The Embryo Before Its Collection?mentioning

confidence: 93%

Non-invasive preimplantation genetic testing for aneuploidies: an update

Navarro-Sánchez

García-Pascual

Rubio

et al. 2022

Reproductive BioMedicine Online

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Aneuploidy is common among preimplantation human embryos used in assisted reproductive technology. Because abnormal chromosome number can negatively affect reproductive outcome, in vitro-fertilized embryos routinely undergo aneuploidy testing before transfer into the uterus. This testing typically involves an invasive trophectoderm biopsy of a blastocyst-stage embryo. However, emerging evidence indicates that, during in vitro development, embryos secrete cell-free DNA into their culture medium; this phenomenon suggests the potential for an alternative, noninvasive assay for aneuploidy. Embryonic cell-free DNA-based assays exhibit high concordance with trophectoderm biopsies, inner cell mass and the whole blastocyst. Yet, informativity and concordance rates may be influenced by several factors: culture day when medium is collected, contamination with external and/or cumulus cell DNA, and previous manipulation of the embryos. In this review, we discuss non-invasive embryonic cell-free DNA analysis as a biomarker to prioritize blastocysts for transfer to help increase implantation rates and reduce miscarriage rates and time to achieve pregnancy. We also discuss ongoing research on the mechanisms underlying embryonic cell-free DNA secretion and how this impacts its role as a biomarker of aneuploidy.

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Blasts from the past: is morphology useful in PGT-A tested and untested frozen embryo transfers?

Cited by 25 publications

References 42 publications

Noninvasive preimplantation genetic testing for aneuploidy in spent culture medium as a substitute for trophectoderm biopsy

Noninvasive preimplantation genetic testing for aneuploidy in spent culture medium as a substitute for trophectoderm biopsy

The correlation between morphological parameters and the incidence of de novo chromosomal abnormalities in 3238 biopsied blastocysts

Non-invasive preimplantation genetic testing for aneuploidies: an update

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