Blastocyst outcomes after sequential media culture vs single step media culture in a human IVF program

Calle, Juan Felipe Vélez de la; Pfeffer, Jérôme; Taar, J.P.; Prigent, Yann

doi:10.1016/j.fertnstert.2013.07.1192

Cited by 5 publications

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“…We further examined 23 studies for eligibility: -A total of three studies were excluded: Two studies used Fertilization and then Cleavage Medium and not a system for Cleavage and then Blastocyst [20,21], and one study was excluded because a different oxygen tension was used in the different groups [22]. -A total of 20 studies were included in this review: Ten studies that randomized oocytes/zygotes [23][24][25][26][27][28][29][30][31][32] and two studies that randomized women [33,34] were included in the forest plots; however, six studies that randomized oocytes/zygotes [35][36][37][38][39][40] and two studies that randomized women [41,42] were not included in the forest plots because it was not possible to extract data for the metaanalyses.…”

Section: Study Selectionmentioning

confidence: 99%

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Sfontouris

Martins

Nastri

et al. 2016

J Assist Reprod Genet

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Purpose The purpose of this study was to undertake a review of the available evidence comparing the use of a single medium versus sequential media for embryo culture to the blastocyst stage in clinical IVF. Methods We searched the Cochrane Central, PubMed, Scopus, ClinicalTrials.gov, Current Controlled Trials and WHO International Clinical Trials Registry Platform to identify randomized controlled trials comparing single versus sequential media for blastocyst culture and ongoing pregnancy rate. Included studies randomized either oocytes/zygotes or women. Eligible oocyte/zygote studies were analyzed to assess the risk difference (RD) and 95 % confidence intervals (CI) between the two media systems; eligible woman-based studies were analyzed to assess the risk ratio (RR) and 95 % CI for clinical pregnancy rate. Results No differences were observed between single and sequential media for either ongoing pregnancy per randomized woman (relative risk (RR) = 0.9, 95 % CI = 0.7 to 1.3, two studies including 246 women, I 2 = 0 %) or clinical pregnancy per randomized woman (RR = 1.0, 95 % CI = 0.7 to 1.4, one study including 100 women); or miscarriage per clinical pregnancy: RR = 1.3, 95 % CI = 0.4 to 4.3, two studies including 246 participants, I 2 = 0 %). Single media use was associated with an increase blastocyst formation per randomized oocyte/ zygote (relative distribution (RD) = +0.06, 95 % CI = +0.01 to Declaration of Authors' Roles Conception and design (CR, LR, WPM, IAS, and NR-F); search strategy (WPM, IAS); data extraction (WPM, PAN, IGRV, CON), analysis, and interpretation (all authors); writing the article (all authors). All authors had full access to all the data in the study and approved the final manuscript.Capsule In conclusion, there is insufficient evidence to recommend either sequential or single-step media as being superior for the culture of embryos to days 5/6 blastocyst stage. +0.12, ten studies including 7455 oocytes/zygotes, I 2 = 83 %) but not top/high blastocyst formation (RD = +0.05, 95 % CI = −0.01 to +0.11, five studies including 3879 oocytes/zygotes, I 2 = 93 %). The overall quality of the evidence was very low for all these four outcomes. Electronic supplementary materialConclusions Although using a single medium for extended culture has some practical advantages and blastocyst formation rates appear to be higher, there is insufficient evidence to recommend either sequential or single-step media as being superior for the culture of embryos to days 5/6. Future studies comparing these two media systems in well-designed trials should be performed.

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Section: Study Selectionmentioning

confidence: 99%

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Sfontouris

Martins

Nastri

et al. 2016

J Assist Reprod Genet

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“…Furthermore, it might decrease the stress caused to embryos by pipetting and variations in temperature and pH ( Reed et al , 2009 ). Avoiding embryo manipulation is a top priority in in vitro cultures to mitigate possible epigenetic risks ( Velez de la Calle et al , 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Embryo development until blastocyst stage with and without renewal of single medium on day 3

Peña¹,

Dávalos²,

Rechkemmer³

et al. 2018

JBRA Assisted Reproduction

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ObjectiveThis study aimed to assess the effects of using a single culture medium not renewed on day 3 on the development of the embryo up to the blastocyst stage.MethodsThe study was carried out in the Assisted Reproduction Laboratory of Clínica Miraflores in the period ranging from April to December of 2016. The study included 589 human embryos obtained from 82 couples submitted to IVF/ICSI with donor oocytes. The couples were randomly divided into two groups: group 1 (medium renewed on day 3) and group 2 (medium not renewed on day 3).ResultsSignificant differences in pregnancy rates, implantation rates, and blastocyst formation were not observed.ConclusionNo statistically significant difference was found between the groups with respect to the analyzed parameters. However, a non-significant trend was observed in favor of the group without medium renewal for clinical pregnancy and positive pregnancy rates (β-hCG).

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Undisturbed culture: a clinical examination of this culture strategy on embryo in vitro development and clinical outcomes

Valera,

Garg,

Bori

et al. 2024

Fertility and Sterility

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Blastocyst outcomes after sequential media culture vs single step media culture in a human IVF program

Cited by 5 publications

References 0 publications

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Blastocyst culture using single versus sequential media in clinical IVF: a systematic review and meta-analysis of randomized controlled trials

Embryo development until blastocyst stage with and without renewal of single medium on day 3

Undisturbed culture: a clinical examination of this culture strategy on embryo in vitro development and clinical outcomes

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