Bladder urinary oxygen tension is correlated with urinary microbiota composition

Shannon, Megan; Limeira, Roberto; Johansen, Danielle; Gao, Xiang; Lin, Huaiying; Dong, Qunfeng; Wolfe, Alan J.

doi:10.1007/s00192-019-03931-y

Cited by 15 publications

(12 citation statements)

References 20 publications

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“…Arterial blood, for example, exhibits an observed range of oxygen tension of 75 to 100 mm Hg in healthy adults, while the UT oxygen tension ranges from 0.47 to 51.5 mm Hg (Fig. 1) (69,85,86). Shannon et al recently reported that variation in the bladder urinary oxygen tension is correlated with compositional changes in the urinary microbiota (69).…”

Section: The Human Urinary Microbiome: An Unexpected Niche Comes Into Focusmentioning

confidence: 99%

“…Urine pH varies among individuals and is usually acidic, although healthy urine pH can range from 5 to 8 (82)(83)(84). Given that many bacteria possess strictly aerobic or anaerobic metabolism, oxygen availability in the UT may play a role in shaping the ecology and spatial organization of the UT microbiota (69,85,86). Arterial blood, for example, exhibits an observed range of oxygen tension of 75 to 100 mm Hg in healthy adults, while the UT oxygen tension ranges from 0.47 to 51.5 mm Hg (Fig.…”

Section: The Human Urinary Microbiome: An Unexpected Niche Comes Into Focusmentioning

confidence: 99%

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Advances in Understanding the Human Urinary Microbiome and Its Potential Role in Urinary Tract Infection

Neugent

Hulyalkar

Nguyen

et al. 2020

mBio

188

145

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Recent advances in the analysis of microbial communities colonizing the human body have identified a resident microbial community in the human urinary tract (UT). Compared to many other microbial niches, the human UT harbors a relatively low biomass. Studies have identified many genera and species that may constitute a core urinary microbiome. However, the contribution of the UT microbiome to urinary tract infection (UTI) and recurrent UTI (rUTI) pathobiology is not yet clearly understood. Evidence suggests that commensal species within the UT and urogenital tract (UGT) microbiomes, such as Lactobacillus crispatus, may act to protect against colonization with uropathogens. However, the mechanisms and fundamental biology of the urinary microbiome-host relationship are not understood. The ability to measure and characterize the urinary microbiome has been enabled through the development of next-generation sequencing and bioinformatic platforms that allow for the unbiased detection of resident microbial DNA. Translating technological advances into clinical insight will require further study of the microbial and genomic ecology of the urinary microbiome in both health and disease. Future diagnostic, prognostic, and therapeutic options for the management of UTI may soon incorporate efforts to measure, restore, and/or preserve the native, healthy ecology of the urinary microbiomes.

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Section: The Human Urinary Microbiome: An Unexpected Niche Comes Into Focusmentioning

confidence: 99%

Section: The Human Urinary Microbiome: An Unexpected Niche Comes Into Focusmentioning

confidence: 99%

Advances in Understanding the Human Urinary Microbiome and Its Potential Role in Urinary Tract Infection

Neugent

Hulyalkar

Nguyen

et al. 2020

mBio

188

145

View full text Add to dashboard Cite

show abstract

“…Similar to the uropathogenic Escherichia coli ( Sokurenko et al., 1998 ), some members of the urinary microbial community could use type 1 fimbriae to bind to specific urothelial proteins, uroplakins, using them as binding sites. Other factors such as pH, oxygen tension or nutrient availability have been described as key determinants; changes in oxygen tension have been associated with alterations in the urinary microbiota ( Shannon et al., 2019 ). In addition, some members of the urinary microbial community, such as Lactobacillus or Streptococcus , have glycosaminoglycan-degrading enzymes, being able to metabolize the urothelium layer and favoring the availability of sugars ( Kawai et al., 2018 ).…”

Section: Urinary Microbiome or Urobiomementioning

confidence: 99%

Urinary Microbiome: Yin and Yang of the Urinary Tract

Pérez-Carrasco

Soriano‐Lerma

Soriano

et al. 2021

Front. Cell. Infect. Microbiol.

127

119

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The application of next generation sequencing techniques has allowed the characterization of the urinary tract microbiome and has led to the rejection of the pre-established concept of sterility in the urinary bladder. Not only have microbial communities in the urinary tract been implicated in the maintenance of health but alterations in their composition have also been associated with different urinary pathologies, such as urinary tract infections (UTI). Therefore, the study of the urinary microbiome in healthy individuals, as well as its involvement in disease through the proliferation of opportunistic pathogens, could open a potential field of study, leading to new insights into prevention, diagnosis and treatment strategies for urinary pathologies. In this review we present an overview of the current state of knowledge about the urinary microbiome in health and disease, as well as its involvement in the development of new therapeutic strategies.

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“…Three The hypervariable region targeted by the primer was V4 solely in 17 studies [20,22,30,[32][33][34][35]38,[40][41][42][43]45,48,49,55,56]; V3 in one study [28]; V6 in two studies [31,44]; V9 in one study [27]; V3-V4 in four studies [21,37,50,54]; V1-V3 in one study [19]; V1-V2 in one study [29]; V3-V5 in one study [39]; V4-V6 in one study [36]. One study used primers targeting V2, 4, 8 and V3, 6, 7, 9 regions [24], and one study targeted V1-3 and V4-6 regions [26].…”

Section: Mitigating the Impact Of Contamination During Dna Extraction Sequencing And Data Analysismentioning

confidence: 99%

“…negative controls were reported in 11 studies (1070 patients-35.6%)[20,25,26,36,40,45,47,48,50,52]. Two negative controls were used in 11 studies (885 patients-29%)[19,[29][30][31][32]34,35,42,44,53,56]. One negative control was used in two studies (73 patients-2.9%)[39,41,55].…”

mentioning

confidence: 99%

Urinary Microbiota—Are We Ready for Prime Time? A Literature Review of Study Methods’ Critical Steps in Avoiding Contamination and Minimizing Biased Results

et al. 2020

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Within the last few years, there have been an increased number of clinical studies involving urinary microbiota. Low-biomass microbiome sequencing (e.g., urine, lung, placenta, blood) is easily biased by contamination or cross-contamination. So far, a few critical steps, from sampling urine to processing and analyzing, have been described (e.g., urine collection modality, sample volume size, snap freezing, negative controls usage, laboratory risks for contamination assessment, contamination of negative results reporting, exploration and discussion of the impact of contamination for the final results, etc.) We performed a literature search (Pubmed, Scopus and Embase) and reviewed the published articles related to urinary microbiome, evaluating how the aforementioned critical steps to obtain unbiased, reliable results have been taken or have been reported. We identified different urinary microbiome evaluation protocols, with non-homogenous reporting systems, which can make gathering results into consistent data for similar topics difficult and further burden the already so complex emerging field of urinary microbiome. We concluded that to ease the progress in this field, a joint approach from researchers, authors and publishers would be necessary in order to create mandatory reporting systems which would allow to recognize pitfalls and avoid compromising a promising field of research.

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Bladder urinary oxygen tension is correlated with urinary microbiota composition

Cited by 15 publications

References 20 publications

Advances in Understanding the Human Urinary Microbiome and Its Potential Role in Urinary Tract Infection

Advances in Understanding the Human Urinary Microbiome and Its Potential Role in Urinary Tract Infection

Urinary Microbiome: Yin and Yang of the Urinary Tract

Urinary Microbiota—Are We Ready for Prime Time? A Literature Review of Study Methods’ Critical Steps in Avoiding Contamination and Minimizing Biased Results

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