Bladder function in a cannabinoid receptor type 1 knockout mouse

Abstract

Objective• To evaluate bladder function in an established cannabinoid type 1 (CB1) receptor knockout (KO) mouse model via organ-bath (in vitro) and urodynamic (cystometric; in vivo) experiments. Materials and Methods• In all, 20 8-week-old female wildtype (WT) mice (C57BL/6) and 20 age-matched CB1 KO mice were used. • Six mice from each group were used for the organ-bath experiments, where the contractile responses of bladder tissue strips after carbachol exposure (carbachol concentration response curve [CCRC]; myogenic contraction) and during electrical field stimulation (EFS; neurogenic contraction) were assessed.• In all, 14 mice per group were used for cystometric experiments without any anaesthesia, in which standard urodynamic variables were assessed 3 days after bladder catheterisation. Results• The CCRCs of bladder strips from CB1 KO mice were similar to those of WT mice. However, during EFS the bladder strips from the CB1 KO mice had significantly lower contractile responses than WT preparations, Conclusions• In vitro, bladder strips from CB1 KO mice responded to muscarinic receptor stimulation similarly as the WT controls, but were less responsive to electrical stimulation of nerves. In vivo, CB1 KO mice had a higher micturition frequency and more spontaneous activity than WT mice.• The present findings suggest that CB1 receptors are involved in peripheral and central nervous control of micturition.

Expression of cannabinoid 1 and, 2 receptors and the effects of cannabinoid 1 and, 2 receptor agonists on detrusor overactivity associated with bladder outlet obstruction in rats

“…They also suggested that CB2 receptor expression did not increase at the onset of DO or an inflammatory response because CB2 receptors are mainly expressed in peripheral immune cells; our results support their hypothesis. In a study using CB1 receptor-knockout mice, Füllhase et al reported that the pathophysiologic role of CB1 receptors, but not that of CB2 receptors, is related to central and peripheral nervous control of micturition [ 19 , 20 ].…”

Serotonin Receptor 5-HT3A Affects Development of Bladder Innervation and Urinary Bladder Function

“…At least six reproducible micturition cycles were recorded and used to quantify multiple parameters. Specifically, intercontractile interval (ICI, time between micturition events), maximal voiding pressure (VP), bladder capacity (BC, defined as the saline infusion rate mu ltiplied by the intercontractile interval), void volume (VV), and non-voiding bladder contraction (NVC, intravesical pressure increases >5 mmHg during the filling phase, without the release of fluid from the urethra) and voiding efficiency (VE, voided volume/bladder capacity × 100) were determined (Vera and Nadelhaft, 2001 ; Füllhase et al, 2013 ; Lai et al, 2016 ; Wu et al, 2016 ). Estrous cycle phase in female mice was not recorded prior to conducting cystometry.…”

Prognostic scores based on the preoperative plasma fibrinogen and serum albumin level as a prognostic factor in patients with upper urinary tract urothelial carcinoma

“…However, reports on the preoperative prognostic factors are still limited in UTUC. Some groups illuminated certain prognostic indexes or models to predict the clinical outcome of UTUC [ 6 , 7 ]. Increasing evidence shows that hemostatic factors, nutritional deficiencies and systemic inflammatory response (SIR) might play an important role in the development and progress of human cancer [ 8 ].…”