Blackcurrant Alleviates Dextran Sulfate Sodium (DSS)-Induced Colitis in Mice

Abstract

Previous studies have reported that anthocyanin (ACN)-rich materials have beneficial effects on ulcerative colitis (UC). Blackcurrant (BC) has been known as one of the foods rich in ACN, while studies demonstrating its effect on UC are rare. This study attempted to investigate the protective effects of whole BC in mice with colitis using dextran sulfate sodium (DSS). Mice were orally given whole BC powder at a dose of 150 mg daily for four weeks, and colitis was induced by drinking 3% DSS for six days. Whole BC relieved symptoms of colitis and pathological changes in the colon. The overproduction of pro-inflammatory cytokines such as IL-1β, TNF-α, and IL-6 in serum and colon tissues was also reduced by whole BC. In addition, whole BC significantly lowered the levels of mRNA and protein of downstream targets in the NF-κB signaling pathway. Furthermore, BC administration increased the expression of genes related to barrier function: ZO-1, occludin, and mucin. Moreover, the whole BC modulated the relative abundance of gut microbiota altered with DSS. Therefore, the whole BC has demonstrated the potential to prevent colitis through attenuation of the inflammatory response and regulation of the gut microbial composition.

Plant-based milk alleviates antibiotics-induced intestinal barrier damage associated with modulation of gut microbiome and metabolome

“…It is widely believed that anti-inflammatory cytokines play a crucial role in regulating the function between the intestinal barrier and intestinal epithelium, whereby increased intestinal permeability allows for a malfunctioning of the immune response to intestinal bacterial antigens, which in turn triggers intestinal inflammation [46,52] . Damage to intestinal epithelial cells caused by CS was reported to worsen the inflammatory response by increasing the generation of pro-inflammatory cytokines [15] , such as TNF-α, IL-1β and IL-6.…”

The Effects of Synbiotics on Dextran-Sodium-Sulfate-Induced Acute Colitis: The Impact of Chitosan Oligosaccharides on Endogenous/Exogenous Lactiplantibacillus plantarum

“…Abnormal immune responses in the gut are a feature of colitis [35], which is characterized by the increase of inflammatory cytokines (TNF-α, IL-1β, IL-6, etc) and decrease of anti-inflammatory cytokines, such as IL-10, and these changes are observed in patients with IBD [36] and DSS-induced colitis mice [37]. The results of the current study revealed the changes in cytokine levels resulting from immunological abnormalities caused by DSS in the gut were attenuated by L. plantarum, COS, and the synbiotics.…”

Penetapan Kadar Antosianin Ekstrak Etanol Bunga Telang (Clitoria ternatea) dengan Metode Differensial pH

“…Antosianin merupakan golongan flavonoid yang banyak terdapat pada buah dan sayuran berwarna gelap, antara lain kubis ungu, anggur ungu, blueberry, ceri, raspberry, beras hitam (D. Li et al 2015), banyak terkandung pada ubi jalar ungu (Rahmah, Ramdan, and Lestari 2023). Tanaman blackcurrant kaya akan kandungan antosianin (Moon, Cha, and Kim 2023). Antosianin merupakan pigmen berwarna yang larut dalam air yang termasuk dalam kelompok fenolik.…”