The Effects of Synbiotics on Dextran-Sodium-Sulfate-Induced Acute Colitis: The Impact of Chitosan Oligosaccharides on Endogenous/Exogenous Lactiplantibacillus plantarum

Abstract

In this work, Lactiplantibacillus plantarum (L. plantarum) isolated from mice feces (LP-M) and pickles (LP-P) were chosen as the endogenous and exogenous L. plantarum, respectively, which were separately combined with chitosan oligosaccharides (COS) to be synbiotics. The anti-inflammatory activity of LP-M, LP-P, COS, and the synbiotics was explored using dextran-sodium-sulfate (DSS)-induced acute colitis mice, as well as by comparing the synergistic effects of COS with LP-M or LP-P. The results revealed that L. plantarum, COS, and the synbiotics alleviated the symptoms of mice colitis and inhibited the changes in short-chain fatty acids (SCFAs), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, IL-10, and myeloperoxidase (MPO) caused by DSS. In addition, the intervention of L. plantarum, COS, and the synbiotics increased the relative abundance of beneficial bacteria Muribaculaceae and Lactobacillus and suppressed the pathogenic bacteria Turicibacter and Escherichia-Shigella. There was no statistically difference between LP-M and the endogenous synbiotics on intestinal immunity and metabolism. However, the exogenous synbiotics improved SCFAs, inhibited the changes in cytokines and MPO activity, and restored the gut microbiota more effectively than exogenous L. plantarum LP-P. This indicated that the anti-inflammatory activity of exogenous LP-P can be increased by combining it with COS as a synbiotic.

Soluble dietary fibers from solid-state fermentation of wheat bran by the fungus Cordyceps cicadae and their effects on colitis mice

“…50 In addition, SCFAs further assisted in enhancing the intestinal barrier function and maintaining the balance of the intestinal microbiota. 51,52 Numerous studies have shown that the short-chain fatty acid content is decreasing in DSS-induced colitis mice. 28,53 This is consistent with the results of our study.…”

Alleviating effect of selenium-enriched Lactobacillus plantarum 6076 on dextran sulfate sodium-induced colitis and liver inflammation in mice

“…37,38 The HS intervention group showed an increase in the relative abundance of Dubosiella, which is consistent with the effect of prebiotics on DSS-induced acute colitis. 39 Dubosiella, an anaerobic Gram-positive bacterial genus, is believed to possess multifaceted benefits, such as regulating metabolism, enhancing intestinal immune function, and resisting inflammatory diseases. 40 Furthermore, similarities observed in the gut microbiota between the control group and the HS group imply that SL may enhance intestinal health by modulating the structure of the gut microbiota and constraining the activity of pathogenic bacteria in the intestinal tract.…”

A Keystone Gut Bacterium Christensenella minuta—A Potential Biotherapeutic Agent for Obesity and Associated Metabolic Diseases

“…It has been intrinsically linked to metabolic diseases such as type 2 diabetes mellitus, obesity, and inflammatory bowel disease [ 11 , 12 , 13 ]. Therefore, maintaining a favorable equilibrium in the gut microbiome is beneficial for host health [ 14 , 15 ].…”