Black Soldier Fly (Hermetia illucens) Larvae Significantly Change the Microbial Community in Chicken Manure

Zhang, Xingxiao; Zhang, Junzhe; Jiang, Linlin; Yu, Xin; Zhu, Hongwei; Zhang, Jianlong; Feng, Zhibin; Zhang, Xiang; Chen, Guozhong; Zhang, Zhijian

doi:10.1007/s00284-020-02276-w

Cited by 30 publications

(34 citation statements)

References 37 publications

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“…It is associated not only with complete elimination of the mycelial forms but also with a decrease in the number of unicellular fungi: the diversity of unicellular fungi reduced from 8 ( Meyerozyma , Diutina , Apiotrichum , Vishniacozyma , Pichia , Trichosporon , Rhodotorula , and Sporobolomyces ) to 2 ( Diutina and Pichia ) genera. Some authors associate the decrease in the diversity of the BSFL substrate microbiome with antimicrobial substances secreted by the larvae, 28,29 whereas others believe that the reduction in the community's alpha diversity is related to the antimicrobial effect of Aspergillus and Penicillum 10 . However, the latter seems unlikely, because we showed the same tendency in the absence of representatives of these genera.…”

Section: Discussionmentioning

confidence: 57%

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Dramatic effect of black soldier fly larvae on fungal community in a compost

et al. 2021

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BACKGROUND Black soldier fly larvae (BSFL) offer very good prospects for the production of cheap and high‐quality dietary protein. This insect is able to consume low‐quality substrates, including food waste. The properties and safety of the compost obtained are largely determined by its microbiome. However, while the bacterial component of the BSFL‐compost microbiome has been much studied, little is known about its fungal component. In plant‐based rearing substrates both the biomass and the metabolic activity of fungi often exceed the biomass and the activity of prokaryotes. So, the purpose of this study was to investigate the fungal community of the compost produced by BSF larvae reared on a food waste substrate. RESULTS Community structure was determined by metabarcoding of the ITS (internal transcribed spacer) region. Species composition and abundance were determined using the plating technique and subsequent identification of the isolated pure cultures. It was found that the primary mycobiome of the food waste substrate that was used consisted of 19 families, represented mainly by phytopathogenic and endophytic genera. Larva incubation led to the complete elimination of all mycelial fungi from the resulting compost. The final mycobiome consisted of only two yeast species, Pichia kudriavzevii and Diutina rugosa, with a total abundance of 1.2 × 107 CFU g−1. CONCLUSIONS The rearing of BSFL led to the complete elimination of mycelial fungi from its feed substrate. The final compost lacked harmful fungi, including molds. This information may be crucial for BSF compost utilization. The phenomenon was also an interesting aspect of zoomicrobial interactions in nature and agriculture. © 2021 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 57%

“…The resulting microbiome may play a decisive role in the quality of compost and future feed. Much attention is currently being given to the prokaryotic component of such substrates 6‐10 . However, such microbiomes consist only to a small extent of prokaryotes, and to a greater extent, of fungi 11 .…”

Section: Introductionmentioning

confidence: 99%

Dramatic effect of black soldier fly larvae on fungal community in a compost

et al. 2021

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“…Previous studies demonstrated that BSFL can grow well on chicken litter ( 29 , 30 ). At the same time, the larvae can change the microbial community of the manure ( 31 ) and even reduce specific food pathogens, such as Escherichia coli , Salmonella , or S. aureus , when growing on manure ( 32 , 33 ). Rearing BSFL on manure is currently not allowed in Europe, however, as defined in Regulation (EC) No.…”

Section: Discussionmentioning

confidence: 99%

Staphylococcus aureus in Substrates for Black Soldier Fly Larvae (Hermetia illucens) and Its Dynamics during Rearing

et al. 2021

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“…This capability is influenced by the presence and abundance of specific bacterial taxa within the gastrointestinal tract that are fundamental for bioconversion processes ( 28 , 29 ). The nutritional versatility of BSFs results in a rich and diverse bacterial microbiome ( 30 , 31 ), with widespread potential for complex bacterial networking ( 32 ). We compared the growth and development of BSFs under two different conditions of nutrient restriction (NR), namely, fresh fruit and vegetable NR diets (NRF and NRV, respectively), and a normal full-nutrient (FN) diet.…”

Section: Introductionmentioning

confidence: 99%