Black Carbon Content in Airway Macrophages is Associated with Reduced CD80 Expression and Increased Exacerbations in Former Smokers With COPD

Tejwani, Vickram; Moughames, Eric; Suresh, Karthik; Tang, Shih En; Mair, Laura G.; Romero, Karina; Putcha, Nirupama; Alexis, Neil E.; Woo, Han; D’Alessio, Franco R.; Hansel, Nadia N.

doi:10.15326/jcopdf.2020.0170

Cited by 3 publications

(8 citation statements)

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“…This study also identified a similar association with greater odds of a severe exacerbation. Furthermore, consistent with that prior study of former smokers with COPD [15] and a study by Kulkarni et al of children with asthma, [32] we identified an association with worse lung function. There is no established MCID for FEV 1 % predicted, however regulators have noted a change of 5-10% of change from baseline is considered to be clinically important [35].…”

Section: Discussionsupporting

confidence: 92%

“…Compared to other studies of AM BC obtained via induced sputum, [14,15,32] participants from this study had a higher observed BC deposition. This may be because those studies were of children or former smokers with COPD.…”

Section: Discussioncontrasting

confidence: 74%

“…The impact of AM BC area on respiratory disease morbidity in COPD has been largely unexplored beyond a small single center study of former smokers with COPD demonstrating an association with increased risk of severe exacerbations and nominally worse lung function which did not identify an association with other COPD outcomes [15]. This study also identified a similar association with greater odds of a severe exacerbation.…”

Section: Discussionmentioning

confidence: 65%

“…In single center studies, inhaled smoke from combusted biomass and indoor PM have been linked to higher AM BC in healthy volunteers and former smokers with COPD, respectively [13,14]. We also recently reported an association of AM BC area with increased severe exacerbations and nominally worse lung function among a small cohort of former smokers with COPD [15].…”

Section: Introductionmentioning

confidence: 77%

“…Given the increase in overall macrophage count, there will be an increase in total carbon and therefore AM carbon effect may be amplified in individuals with COPD. Prior studies have typically utilized the mean carbon area per macrophage, [13][14][15]20] which does not account for variation in macrophage area. However, a recent study assessing both BC area and BC percent identified stronger associations between seven day PM 2.5 exposures and BC percent (compared to BC area) [38].…”

Section: Discussionmentioning

confidence: 99%

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Black carbon content in airway macrophages is associated with increased severe exacerbations and worse COPD morbidity in SPIROMICS

et al. 2022

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Background Airway macrophages (AM), crucial for the immune response in chronic obstructive pulmonary disease (COPD), are exposed to environmental particulate matter (PM), which they retain in their cytoplasm as black carbon (BC). However, whether AM BC accurately reflects environmental PM2.5 exposure, and can serve as a biomarker of COPD outcomes, is unknown. Methods We analyzed induced sputum from participants at 7 of 12 sites SPIROMICS sites for AM BC content, which we related to exposures and to lung function and respiratory outcomes. Models were adjusted for batch (first vs. second), age, race (white vs. non-white), income (<$35,000, $35,000~$74,999, ≥$75,000, decline to answer), BMI, and use of long-acting beta-agonist/long-acting muscarinic antagonists, with sensitivity analysis performed with inclusion of urinary cotinine and lung function as covariates. Results Of 324 participants, 143 were current smokers and 201 had spirometric-confirmed COPD. Modeled indoor fine (< 2.5 μm in aerodynamic diameter) particulate matter (PM2.5) and urinary cotinine were associated with higher AM BC. Other assessed indoor and ambient pollutant exposures were not associated with higher AM BC. Higher AM BC was associated with worse lung function and odds of severe exacerbation, as well as worse functional status, respiratory symptoms and quality of life. Conclusion Indoor PM2.5 and cigarette smoke exposure may lead to increased AM BC deposition. Black carbon content in AMs is associated with worse COPD morbidity in current and former smokers, which remained after sensitivity analysis adjusting for cigarette smoke burden. Airway macrophage BC, which may alter macrophage function, could serve as a predictor of experiencing worse respiratory symptoms and impaired lung function.

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Section: Discussionsupporting

confidence: 92%

Section: Discussioncontrasting

confidence: 74%

Section: Discussionmentioning

confidence: 65%

Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

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Black carbon content in airway macrophages is associated with increased severe exacerbations and worse COPD morbidity in SPIROMICS

et al. 2022

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Airway and Systemic Prostaglandin E2 Association with COPD Symptoms and Macrophage Phenotype

Tejwani¹,

Villabona-Rueda²,

Khare³

et al. 2023

J COPD F

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The Effects of Ginkgo biloba Extract on Autophagy in Human Macrophages Stimulated by Cigarette Smoke Extract

Zhang¹,

Zhu²,

Zhang³

et al. 2023

Front. Biosci. (Landmark Ed)

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Objective: To investigate the effects of Ginkgo biloba extract (GBE) on autophagy in human macrophages stimulated by cigarette smoke extract (CSE). Methods: The human monocyte cell line U937 was cultured in vitro, and phorbol ester (PMA) was added to the cell culture medium to induce differentiation into human macrophages. CSE was prepared by traditional methods for experiments. The cells were divided into four groups: the blank group, the CSE model group, the GBE + CSE group, and the rapamycin + CSE group. Immunofluorescence was used to identify human macrophages, transmission electron microscopy was used to observe the ultrastructure of human macrophages in each group, ELISA was used to measure the amount of IL-6 and IL-10 in the supernatant from each group of cells, the mRNA levels of p62, ATG5, ATG7, and Rab7 were measured by real-time qPCR, and the protein expression levels of p62, ATG5, ATG7, and Rab7 were measured by Western blotting. Results: U937 cells were successfully differentiated into human macrophages after induction with PMA. The CSE model group had many more autophagosomes than the blank group. Compared with the CSE model group, the GBE + CSE group and the rapamycin + CSE group had significantly more autophagolysosomal. Compared with the other groups, the CSE model group had a higher level of IL-6 but a lower level of IL-10 in the supernatant (p < 0.05). Compared with the blank group, the mRNA and protein expression levels of p62 in the CSE model group were significantly decreased, while the mRNA and protein expression levels of ATG5 and ATG7 were significantly increased in the CSE model group (p < 0.05). No difference was found in the mRNA and protein expression levels of Rab7 between the blank group and the CSE model group. Compared with the CSE model group, the IL-6 level in the GBE + CSE group and the rapamycin + CSE group cell culture supernatant decreased significantly, p62 mRNA and protein expression significantly decreased, while ATG5, ATG7, and Rab7 mRNA and protein expression levels were significantly increased (p < 0.05). Moreover, increased LC3-II/LC3-I ratio were also found in the GBE + CSE group and the rapamycin + CSE group compared with the CSE model group. Conclusions: GBE could promote the fusion of autophagosomes and lysosomes in human macrophages, enhance the autophagy function of human macrophages, and reduce the damaging effect of CSE on the autophagy function of macrophages.

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Black Carbon Content in Airway Macrophages is Associated with Reduced CD80 Expression and Increased Exacerbations in Former Smokers With COPD

Abstract: For personal use only. Permission required for all other uses.

Cited by 3 publications

References 36 publications

Black carbon content in airway macrophages is associated with increased severe exacerbations and worse COPD morbidity in SPIROMICS

Black carbon content in airway macrophages is associated with increased severe exacerbations and worse COPD morbidity in SPIROMICS

Airway and Systemic Prostaglandin E2 Association with COPD Symptoms and Macrophage Phenotype

The Effects of Ginkgo biloba Extract on Autophagy in Human Macrophages Stimulated by Cigarette Smoke Extract

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