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Noll, Thomas; Eisenkrätzer, Detlef; Kiesewetter, André; Dinter, André; Zeng, Steffen; Wandrey, Christian; Biselli, M.; Berger, Eric G.

doi:10.1023/a:1015512607352

Cited by 3 publications

(2 citation statements)

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“…The fluidized bed reactor was preferred to a fixed bed reactor for better mass transport of dissolved hydrogen, avoiding concentration gradients over the cross-section of the bed. [27][28][29] Immobilization experiments were carried out with beads of graphite or glass. With graphite beads the reactor could be operated for more than 80 h (number of residence times more than 40) at a low conversion level, ensuring linear correlation of conversion and activity.…”

Section: Resultsmentioning

confidence: 99%

Utilization of adsorption effects for the continuous reduction of NADP⁺with molecular hydrogen by Pyrococcus furiosus hydrogenase

et al. 2003

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The use of hydrogenases for the direct reduction of cofactors with molecular hydrogen provides a means to utilize this clean and strong reducing agent for use in reductive biotransformations. The hydrogenase I from Pyrococcus furiosus is a promising enzyme for the production of NADPH from NADP + with molecular hydrogen. We observed adsorption of the active enzyme by using cyclic voltammetry. Our investigations revealed an electroactive enzyme species adsorbed onto the surface of the working electrode. This facile route for immobilisation of the active enzyme was subsequently utilized for the continuous reduction of NADP + to NADPH with molecular hydrogen in a continuously operated fluidized bed reactor with hydrogenase adsorbed on graphite beads. The reactor was operated at low conversion for more than 40 residence times (80 h). The apparent retention was found to be 98% on unmodified graphite carriers.

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Section: Resultsmentioning

confidence: 99%

Utilization of adsorption effects for the continuous reduction of NADP⁺with molecular hydrogen by Pyrococcus furiosus hydrogenase

et al. 2003

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“…. Antibodies, immunoglobulins and other pharmaceutically important proteins can be manufactured using this technology [9,10].…”

Section: Bubble-free Oxygen Gassingmentioning

confidence: 99%

Membranes in Biotechnology

Lütz¹,

Rao²,

Wandrey³

2006

Chem Eng & Technol

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Membranes have become an integral part of biotechnological processes. They have proved to be useful tools for the retention of biocatalysts in industrial biotransformations. This paper describes the development of membrane reactors and the application of membrane-based processes in biotransformations. Membrane systems for substrate dosing (e.g., aeration), catalyst retention (e.g., ultrafiltration), product separation (e.g., pervaporation), and salt removal (electrodialysis) from solutions are dealt with. The use of membranes in bioelectrochemistry is also discussed.

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Experimental Investigation on Shear Deformation of Fresh Concrete

Li¹,

Li²

2010

Nihon Kenchiku Gakkai Kozokei Ronbunshu

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Cited by 3 publications

References 20 publications

Utilization of adsorption effects for the continuous reduction of NADP⁺with molecular hydrogen by Pyrococcus furiosus hydrogenase

Utilization of adsorption effects for the continuous reduction of NADP⁺with molecular hydrogen by Pyrococcus furiosus hydrogenase

Membranes in Biotechnology

Experimental Investigation on Shear Deformation of Fresh Concrete

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Untitled

Cited by 3 publications

References 20 publications

Utilization of adsorption effects for the continuous reduction of NADP+with molecular hydrogen by Pyrococcus furiosus hydrogenase

Utilization of adsorption effects for the continuous reduction of NADP+with molecular hydrogen by Pyrococcus furiosus hydrogenase

Membranes in Biotechnology

Experimental Investigation on Shear Deformation of Fresh Concrete

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Utilization of adsorption effects for the continuous reduction of NADP⁺with molecular hydrogen by Pyrococcus furiosus hydrogenase

Utilization of adsorption effects for the continuous reduction of NADP⁺with molecular hydrogen by Pyrococcus furiosus hydrogenase