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Banik, Rathindra Mohan; Santhiagu, A.

doi:10.1023/a:1020405217277

Cited by 7 publications

(1 citation statement)

References 12 publications

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“…1 Many metabolites and/or bioproducts present narrow tolerance limits of pH, ionic strength, temperature, osmotic pressure, and surface charges; thus, the extraction and isolation techniques must be specific and compatible with the product. 2 Conventional techniques used for product recovery from biotechnological processes are usually expensive and present low yields. 3 There have been, therefore, considerable efforts from the industrial and academic communities for the development of cost-effective separation techniques, 4 such as liquid-liquid extraction in aqueous biphasic systems (ABS).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Cation Influence on the Formation and Extraction Capability of Ionic-Liquid-Based Aqueous Biphasic Systems

et al. 2009

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In addition to the large range of applications proposed in literature, ionic liquids (ILs) have been recently reported to be able to form aqueous biphasic systems (ABS). They could thus be interesting media in biotechnological applications for the separation and purification of vital biomolecules. Therefore, in this work, a systematic study involving a large number of imidazolium-based ILs was conducted to provide new information related to ILs' ABS-promoting capability and extraction ability. For that purpose, the influence of the number of alkyl groups present at the cation, the cation side alkyl chain length, and the presence of double bonds, aromatic rings, and hydroxyl groups on this alkyl chain were evaluated. Ternary phase diagrams of the ABS formed by these ILs and K 3 PO 4 and the respective tie-lines were measured and presented. The ABS here investigated were further characterized for the first time accordingly to their extractive potential for amino acids, where L-tryptophan was selected as a model biomolecule. The partition coefficients here obtained were shown to be substantially larger than those observed in conventional ABS, demonstrating therefore the fine potential of IL-based ABS for biomolecules separation and purification.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Cation Influence on the Formation and Extraction Capability of Ionic-Liquid-Based Aqueous Biphasic Systems

et al. 2009

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Extractive fermentation in cloud point system for lipase production by Serratia marcescens ECU1010

Pan

Wang

et al. 2009

Appl Microbiol Biotechnol

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Extractive microbial fermentation for production of lipase by Serratia marcescens ECU1010 has been carried out in cloud point system. The cloud point system is composed of mixture nonionic surfactants with a ratio of Triton X-114 to Triton X-45 4:1 in aqueous solution. The lipase prefers to partition into the surfactant rich phase (coacervate phase) whereas the cells and other hydrophilic proteins retain in the dilute phase of cloud point system. Thus, a concentration factor 4.2-fold and a purification factor 1.3-fold of the lipase have been achieved in the extractive fermentation process. This is the first report about extractive fermentation of proteins in cloud point system.

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A comprehensive perspective on sustainable bioprocessing through extractive fermentation: challenges and prospects

Muniasamy¹,

Venkatachalam²,

Rangarajan³

et al. 2023

Rev Environ Sci Biotechnol

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Untitled

Cited by 7 publications

References 12 publications

Evaluation of Cation Influence on the Formation and Extraction Capability of Ionic-Liquid-Based Aqueous Biphasic Systems

Evaluation of Cation Influence on the Formation and Extraction Capability of Ionic-Liquid-Based Aqueous Biphasic Systems

Extractive fermentation in cloud point system for lipase production by Serratia marcescens ECU1010

A comprehensive perspective on sustainable bioprocessing through extractive fermentation: challenges and prospects

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