Untitled

Abstract

Our results indicate that trans-resveratrol-3-O-glucuronide and trans-resveratrol-3-sulfate are the most abundant metabolites of resveratrol. Virtually no unconjugated resveratrol was detected in urine or serum samples, which might have implications regarding the significance of in vitro studies that used only unconjugated resveratrol.

Resveratrol as a Chemopreventive Agent: A Promising Molecule for Fighting Cancer

“…The reaction is catalyzed by UDP-glucuronosyltransferases of the family 1A. Two major metabolites have been characterized by LC-MS on human hepatocytes: trans-resveratrol 3-O-glucuronide and trans resveratrol-3-sulfate [253].…”

“…Interestingly, Walle et al (2004) still detect a blood peak of 1.3 µM after 6 hours, indicating an entero-hepatic recirculation of metabolites reabsorbed after intestinal hydrolysis [246]. Conversely, Yu et al (2002) consider that oral absorption of resveratrol (and not gavage) may increase transiently free resveratrol concentration in the blood via buccal mucosa absorption [253]. Moreover, it is probable that tissue sulfatases and glucuronidases can hydrolyze conjugated resveratrol and lead to higher local tissue concentrations of aglycone.…”

“…As underlined by Yu et al (2002), in vitro studies using unconjugated resveratrol only need to be completed by experiments with metabolites of resveratrol [253]. While bioavailability is better understood in the gut tract and related organs, in vitro and in vivo studies on lung and breast cell/tissue must consider resveratrol metabolism and tissue bioabsorption.…”

Quantitation of trans-resveratrol and detection of its metabolites in human plasma and urine by high performance liquid chromatography

“…Part of the development and refinement of the analytical assay included the validation of a gradient elution HPLC assay for plasma and urine. Several methods have been published for the analysis of resveratrol and some of its metabolites [21,[24][25][26] This method is an improvement on the UV-HPLC method developed by Zhu et al, in 1999 [21] as it allows resolution of the several human phase II metabolites of resveratrol, by direct means rather than by deconjugation, (many of which are very polar and difficult to resolve in gradient modifications of the previous method) in addition to trans-resveratrol and uses a mobile phase that can be directly transferred to LC-MSMS for identification of these metabolites. Wang et al, in 2005 [26] published an HPLC-MSMS method for identification of major metabolites in rat urine, this method identified several conjugated metabolites as well as dihydroresveratrol and its monosulfate conjugate that were found in humans by Walle et al, in 2004 [27].…”

Preparação e caracterização de suspensões coloidais de nanocarreadores lipídicos contendo resveratrol destinados à administração cutânea

“…1,2 Vários estudos têm destacado e comprovado os benefícios do resveratrol à saúde. Esta substância tem demonstrado propriedades quimiopreventivas, [3][4][5][6][7][8] antioxidante, 9,10 antiplaquetária, 11 antifúngica, 12 anti-inflamatória, 13 cardioprotetora, 14,15 entre outras. O resveratrol age como efetor biológico pleiotrópico que regula as três fases do processo da carcinogênese, ou seja, início, promoção e progressão do tumor.…”