Preparação e caracterização de suspensões coloidais de nanocarreadores lipídicos contendo resveratrol destinados à administração cutânea

Nemen, Daiane; Lemos‐Senna, Elenara

doi:10.1590/s0100-40422011000300008

Cited by 32 publications

(15 citation statements)

References 34 publications

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“…The polydispersity index, which provides information about thehomogeneity of the distribution of sizes was low (<0.3) for all samples, indicating the formation of monodisperse systems [32] or narrow range of sizes. The LMC and LEC showed no significant difference (p ≤ 0.05) of the polydispersity index compared to the control (without sample).…”

Section: Particle Size Polydispersity Index and Entrapment Efficiencymentioning

confidence: 91%

Development and Characterization of Nanovesicles Containing Phenolic Compounds of Microalgae Spirulina Strain LEB-18 and Chlorella pyrenoidosa

Assis¹,

Machado²,

Motta³

et al. 2014

AMPC

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The objective of this study was to elaborate liposomes, through the lipid film hydration methodology, to nanoencapsulate phenolic compounds of Spirulina LEB-18 and Chlorella pyrenoidosa microalgae, and evaluate their physicochemical characteristics and storage stability for 21 days. The total phenolic compounds were evaluated using a calibration curve of gallic acid using methanol and ethanol as extraction solvents. The size and polydispersity index of nanovesicles were determined by light scattering and the percentage encapsulation efficiency was determined by a centrifugation process. The stability of the liposomes at storage time was measured by zeta potential for 21 days. The methanol extracts from Spirulina had a higher content of phenolic compounds (2.62 mg gallic acid•g −1 of microalgae) compared to the extracts of Chlorella. However, liposomes with ethanolic extracts of the two algae showed higher encapsulation efficiency. The value was higher (96.40%) for Chlorella. All samples obtained nanometric size, with the highest value obtained for the liposome containing ethanol extract of Chlorella (239 nm) differing significantly (p ≤ 0.05) from the others. The liposomes containing extracts of Spirulina were more stable during the 21 days of storage, whereas, those consisting of ethanol extract showed no significant difference (p ≤ 0.05) throughout this period.

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Section: Particle Size Polydispersity Index and Entrapment Efficiencymentioning

confidence: 91%

Development and Characterization of Nanovesicles Containing Phenolic Compounds of Microalgae Spirulina Strain LEB-18 and Chlorella pyrenoidosa

Assis¹,

Machado²,

Motta³

et al. 2014

AMPC

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“…The NE are kinetically stable due to their steric balance, especially when formulated with nonionic surfactants Gupta et al, 2016). Due to their small size droplets, they are resistant to being physically affected by creaming and sedimentation instability, because their Brownian motion that is sufficient to overcome the force of gravity separation and flocculation (Nam et al, 2010;Wooster, Golding, Sanguansri, 2008;Nemen, Lemos-Senna, 2011;Anton, Benoit, Saulnier, 2008;Yukuyama et al, 2016). The main physical instability of NE is the Ostwald ripening phenomena that consist in an increase in the average radius of the droplets when the droplets on the dispersed phase migrate to continuous phase by the process of mass transfer (Maruno, Rocha-Filho, 2009;Taylor, 2003;Urbina-Villalba et al, 2009).…”

mentioning

confidence: 99%

The use of Brazilian vegetable oils in nanoemulsions: an update on preparation and biological applications

Bajerski

Michels

Colomé

et al. 2016

Braz. J. Pharm. Sci.

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Vegetable oils present important pharmacological properties, which gained ground in the pharmaceutical field. Its encapsulation in nanoemulsions is considered a promising strategy to facilitate the applicability of these natural compounds and to potentiate the actions. These formulations offer several advantages for topical and systemic delivery of cosmetic and pharmaceutical agents including controlled droplet size, protection of the vegetable oil to photo, thermal and volatilization instability and ability to dissolve and stabilize lipophilic drugs. For these reasons, the aim of this review is to report on some characteristics, preparation methods, applications and especially analyze recent research available in the literature concerning the use of vegetable oils with therapeutic characteristics as lipid core in nanoemulsions, specially from Brazilian flora, such as babassu (Orbignya oleifera), aroeira (Schinus molle L.), andiroba (Carapa guaianiensis), casca-de-anta (Drimys brasiliensis Miers), sucupira (Pterodon emarginatus Vogel) and carqueja doce (Stenachaenium megapotamicum) oils.Uniterms: Plant oils/nanoemulsions/preparation. Plant oils/biological applications. INTRODUCTIONThe use of submicrometric colloidal nanocarriers such as nanoparticles (NP) lipid and polymeric, nanoemulsions (NE), microemulsions (ME), liposomes, and polymeric micelles is considered a promising system in the pharmaceutical field, because they have numerous advantages over traditional formulations such as: sustained release of the active element; solubilization of lipophilic molecules; use for different routes of administration; protection from chemical and enzymatic degradation of labile molecules; reduction of vegetable oils (VO) volatilization, side effects and dose (Mäder, Mehnert, 2005;Mehnert, Mäder, 2001;Gref, Couvreour, 2006;Couvreur, Vauthier, 2006; Panyam, Labhasetwar, 2003;Soppimath et al., 2001;Ai et al., 2011; Contri et al., 2012;Contri et al., 2014;Dimer et al., 2014;Severino et al., 2015;Frank et al., 2015;Asbahani et al., 2015). However, all systems differ thermodynamic stability, structure, chemical composition, efficiency encapsulation and types of application (Mäder, Mehnert, 2005;Ai et al., 2011).The development of suitable nanocarriers for pharmaceutical or cosmetic application requires the adequate selection of their adjuvants such as polymers for nanocapsules (NC), surfactants, and oils (Schaffazick et al., 2003;Alvarez-Román et al., 2001;Bouchemal et al., 2004;Friedrich et al., 2008). Recently, special attention has been given to the type of oily phase used as the core in the preparation of NE. VO has been preferred not only due to the concept that it is safe and biocompatible, but mainly because of the diversity of benefits, and the complex composition of fatty acids can exercise under the skin, protecting it against dehydration, solar radiation, inflammation, insect attack, microorganisms, and viruses (Tadros, Kessell, 2004;Bloise, 2003;Oyedeji, Okeke, 2010;Bakkali et. al., 2008;Harris, 2002; Contri et al, ...

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“…All solutions were freshly prepared and filtered through a 0.45-µm membrane prior to HPLC injection. The linearity was determined by linear regression analysis [45].…”

Section: Linearitymentioning

confidence: 99%

Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array

Rigon

Fachinetti

Severino³

et al. 2019

Applied Sciences

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A new method based on reverse-phase HPLC combined with photodiode array (PDA) was developed to quantify the release of trans-resveratrol (tRES) from solid lipid nanoparticles (SLN). The mobile phase was composed of 75:0:25 (V/V) water/methanol/acetonitrile at 0–3.5 min, 32.5:30.0:37.5 (V/V) water/methanol/acetonitrile at 3.6–5.8 min, and 75:0:25 (V/V) water/methanol/acetonitrile at 5.9–10 min. The flow rate was set at 1.0 mL/min, and tRES was detected at the wavelength of 306.6 nm. A concentration range of 1–100 µg/mL was used to obtain the linear calibration curve. SLN were produced by ultrasound technique to load 0.1% (wt/wt) of tRES, and the in vitro release of the drug was run in modified Franz diffusion cells. The mean recovery of tRES was found to be 96.84 ± 0.32%. The intra-assay and inter-assay coefficients of variation were less than 5%. The proposed method was applied to in vitro permeability studies, and the Weibull model was found to be the one that best fits the tRES release, which is characterized by a simultaneous lipid chain relaxation and erosion during drug release.

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Preparação e caracterização de suspensões coloidais de nanocarreadores lipídicos contendo resveratrol destinados à administração cutânea

Cited by 32 publications

References 34 publications

Development and Characterization of Nanovesicles Containing Phenolic Compounds of Microalgae <i>Spirulina</i> Strain LEB-18 and <i>Chlorella pyrenoidosa</i>

Development and Characterization of Nanovesicles Containing Phenolic Compounds of Microalgae <i>Spirulina</i> Strain LEB-18 and <i>Chlorella pyrenoidosa</i>

The use of Brazilian vegetable oils in nanoemulsions: an update on preparation and biological applications

Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array

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Preparação e caracterização de suspensões coloidais de nanocarreadores lipídicos contendo resveratrol destinados à administração cutânea

Cited by 32 publications

References 34 publications

Development and Characterization of Nanovesicles Containing Phenolic Compounds of Microalgae &lt;i&gt;Spirulina&lt;/i&gt; Strain LEB-18 and &lt;i&gt;Chlorella pyrenoidosa&lt;/i&gt;

Development and Characterization of Nanovesicles Containing Phenolic Compounds of Microalgae &lt;i&gt;Spirulina&lt;/i&gt; Strain LEB-18 and &lt;i&gt;Chlorella pyrenoidosa&lt;/i&gt;

The use of Brazilian vegetable oils in nanoemulsions: an update on preparation and biological applications

Quantification of Trans-Resveratrol-Loaded Solid Lipid Nanoparticles by a Validated Reverse-Phase HPLC Photodiode Array

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Development and Characterization of Nanovesicles Containing Phenolic Compounds of Microalgae <i>Spirulina</i> Strain LEB-18 and <i>Chlorella pyrenoidosa</i>

Development and Characterization of Nanovesicles Containing Phenolic Compounds of Microalgae <i>Spirulina</i> Strain LEB-18 and <i>Chlorella pyrenoidosa</i>