Bizionia argentinensis sp. nov., isolated from surface marine water in Antarctica

Abstract

A marine bacterial strain, designated strain JUB59 T , was isolated from surface seawater in Antarctica and subsequently characterized. Cells were found to be Gram-negative, non-motile rods forming butyrous, shiny, yellowish orange colonies on marine agar. Growth occurred at 2-28 6C (optimally at 22-25 6C) but not at 30 6C; Na + ions were required, but 9 % NaCl (w/v) was not tolerated. Phylogenetic analysis, based on comparisons of the complete 16S rRNA gene sequence of the novel isolate with the sequences of closely related strains, showed that strain JUB59 T belonged to the family Flavobacteriaceae, representing a novel species of the genus Bizionia. The highest levels of sequence similarity were found with respect to Bizionia myxarmorum ADA-4 T (97.4 %) and Bizionia algoritergicola APA-1 T (97.1 %). However, the DNA-DNA relatedness of strain JUB59 T with respect to these two strains was low (15.9-17.3 and 19.3-22.1 %, respectively). The predominant fatty acids of strain JUB59 T were iso-15 : 1v10c (18.1 %), iso-15 : 0 (17.3 %), anteiso-15 : 0 (13.9 %), iso-17 : 0 3-OH (9.2 %), 15 : 0 (6.0 %) and iso-16 : 0 3-OH (5.3 %). The main polar lipids were phosphatidylethanolamine, an aminolipid, an amino-positive phospholipid and two unidentified lipids. MK-6 was the major respiratory quinone (.90 %) and the DNA G+C content was 34 mol%. On the basis of the data obtained, strain JUB59 T represents a novel species of the genus Bizionia, for which the name Bizionia argentinensis sp. nov. is proposed. The type strain is JUB59 T (5DSM 19628 T 5CCM-A-29 1259 T ).The family Flavobacteriaceae (Bernardet et al., 1996(Bernardet et al., , 2002 currently comprises more than 40 genera. Many of these genera group strains that have been recovered from a variety of marine habitats (Bernardet & Nakagawa, 2006). These genera form a distinct 'marine clade' in phylogenetic trees based on 16S rRNA gene sequences (Bowman, 2004;Bowman & Nichols, 2005;Bowman, 2006). Marine members of the family occur in tropical, temperate (Nedashkovskaya et al., 2004;Jung et al., 2005;Kwon et al., 2006) and polar (Bowman, 2000;Bowman & Nichols, 2002;Yi et al., 2005) marine environments, where they play an important role in the mineralization of organic matter, especially following algal blooms (Bowman et al., 1997;Pinhassi et al., 2004). The genus Bizionia belongs to the marine clade of the family Flavobacteriaceae. First described by Nedashkovskaya et al. (2005), the genus currently comprises five species, isolated from sea-ice brine and various marine invertebrates: Bizionia paragorgiae (Nedashkovskaya et al., 2005), B. saleffrena, B. gelidisalsuginis, B. algoritergicola and B. myxarmorum (Bowman & Nichols, 2005). In this study, a bacterial strain isolated from seawater in Antarctica was analysed by a polyphasic taxonomic approach and was found to represent a novel member of the genus Bizionia. B. saleffrena HFD T , B. gelidisalsuginis IC164 T , B. algoritergicola APA-1 T and B. myxarmorum ADA-4 T were used as reference strains for all of the physiolog...

Bizionia hallyeonensis sp. nov., isolated from seawater in an oyster farm

“…The genomic DNA G+C content of strain T-y7 T was 37.1 mol%, a value within the range reported for Bizionia species (Nedashkovskaya et al, 2010). Given the phylogenetic inference and the apparent absence of chemotaxonomic properties that permit the novel strain to be distinguished from established members of the genus Bizionia (Nedashkovskaya et al, 2005(Nedashkovskaya et al, , 2010Bowman & Nichols, 2005;Bercovich et al, 2008), it seems appropriate to classify strain T-y7…”

Meridianimaribacter flavus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from marine sediment of the South China Sea

“…With API 20E and 20NE strips, positive for b-glucosidase (5aesculinase) and b-galactosidase activity, but negative for urease, arginine dihydrolase, ornithine decarboxylase, tryptophan deaminase and lysine decarboxylase activities. Jung et al, 2005;Yang & Cho, 2008); 3, Gelidibacter (n54; Kwon et al, 2006;Bowman & Nichols, 2005); 4, Subsaxibacter broadyi P7 T (Bowman & Nichols, 2005;Kwon et al, 2006); 5, Subsaximicrobium (n52; Bowman & Nichols, 2005;Kwon et al, 2006); 6, Yeosuana aromativorans GW1-1 T (Kwon et al, 2006); 7, Algibacter (n52; Nedashkovskaya et al, 2004Nedashkovskaya et al, , 2007; 8, Bizionia (n56; Kwon et al, 2006;Bercovich et al, 2008;Bowman & Nichols, 2005); 9, Formosa (n52; Nedashkovskaya et al, 2006). n, Number of type strains in each genus.…”

“…With API ZYM strips, positive for alkaline phosphatase, esterase lipase (C8), leucine arylamidase, valine arylamidase, a-chymotrypsin, acid phosphatase and naphthol-AS-BI-phosphohydrolase activities, weakly positive for esterase (C4), cystine arylamidase, trypsin and a-glucosidase activities, but negative for lipase (C14), a-galactosidase, b-glucuronidase, N-acetyl-b-glucosaminidase, a-mannosidase and a-fucosidase activities. The following carbon (Kwon et al, 2006);7, Algibacter (n52;Nedashkovskaya et al, 2004Nedashkovskaya et al, , 20078, Bizionia (n56;Bowman & Nichols, 2005;Bercovich et al, 2008);9, Formosa (n52;Nedashkovskaya et al, 2006). n, Number of type strains in each genus.…”

Bizionia echini sp. nov., isolated from a sea urchin

“…Currently, the genus Bizionia comprises the representatives of six recognized species that were isolated from cold or moderately cold marine and saline lake environments (Bowman & Nichols, 2005; Nedashkovskaya et al, 2005;Bercovich et al, 2008). …”