Bizionia echini sp. nov., isolated from a sea urchin

Abstract

A bacterial strain, designated KMM 6177 T , was isolated from the sea urchin Strongylocentrotus intermedius and subjected to a polyphasic taxonomic investigation. The bacterium was found to be heterotrophic, aerobic, non-motile by gliding and orange-pigmented. Comparative phylogenetic analysis based on 16S rRNA gene sequencing placed the marine isolate in the genus Bizionia, a member of the family Flavobacteriaceae, with 16S rRNA gene sequence similarity of 94.9-98.6 % with recognized Bizionia species. Strain KMM 6177T grew at 4-39 6Cand with 1-8 % NaCl. It produced alkaline phosphatase, catalase and oxidase and hydrolysed aesculin, gelatin, DNA and Tween 20. The predominant fatty acids were iso-C 15 : 1 , iso-C 15 : 0 , iso-C 15 : 0 3-OH, iso-C 17 : 0 3-OH and a summed feature (comprising iso-C 15 : 0 2-OH and/or C 16 : 1 v7c). The DNA G+C content was 34.4 mol%. A combination of phylogenetic, genotypic and phenotypic data clearly indicated that strain KMM 6177 T represents a novel species in the genus Bizionia, for which the name Bizionia echini sp. nov. is proposed. The type strain is KMM 6177 T (5KCTC 22015The genus Bizionia, a member of the family Flavobacteriaceae (Bernardet et al., 2002), was created for the accommodation of heterotrophic, strictly aerobic, rodshaped, non-motile by gliding, pigmented and Gramnegative bacteria (Nedashkovskaya et al., 2005). Currently, the genus Bizionia comprises the representatives of six recognized species that were isolated from cold or moderately cold marine and saline lake environments (Bowman & Nichols, 2005; Nedashkovskaya et al., 2005;Bercovich et al., 2008).Here we report on the isolation and characterization of an unknown bacterial strain, designated KMM 6177 T , by using a polyphasic taxonomic approach. According to a comparative phylogenetic analysis based on 16S rRNA gene sequencing, strain KMM 6177 T was placed in the genus Bizionia of the family Flavobacteriaceae, in which it represents a novel species. Strain KMM 6177T was isolated from a sea urchin specimen collected from Troitsa Bay of the Sea of Japan (also known as the East Sea). For strain isolation, 0.1 ml sea urchin tissue homogenate was plated on marine agar 2216 (Difco). After primary isolation and purification, strains were cultivated at 28 uC on the same medium and stored at -80 u C in marine broth (Difco) supplemented with 20 % (v/v) glycerol.The phylogenetic position of strain KMM 6177 T was determined from its almost-complete 16S rRNA gene sequence (1448 bp). Genomic DNA extraction, PCR and sequencing of the 16S rRNA gene were performed by using previously described procedures (Cho et al., 2006). The sequence obtained was aligned with those of representative members of selected genera belonging to the family Flavobacteriaceae by using PHYDIT version 3.1 (http:// plaza.snu.ac.kr/~jchun/phydit/). Phylogenetic trees were inferred by using suitable programs of the PHYLIP package (Felsenstein, 1993). Phylogenetic distances were calculated from the Jukes-Cantor model (Jukes & Cantor, 1969), and the t...

Corallibacter vietnamensis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae

“…Corallibacter vietnamensis gen. nov., sp. nov. Asker et al (2007), Bercovich et al (2008), Bowman & Nichols (2005), Ivanova et al (2004), , Lau et al (2005), Nedashkovskaya et al (2005aNedashkovskaya et al ( , b, 2006aNedashkovskaya et al ( , 2008Nedashkovskaya et al ( , 2009bNedashkovskaya et al ( , 2010, Pinhassi et al (2009), Romanenko et al (2009), Wang et al (2010) and this study.…”

Bizionia hallyeonensis sp. nov., isolated from seawater in an oyster farm

“…The genomic DNA G+C content of strain T-y7 T was 37.1 mol%, a value within the range reported for Bizionia species (Nedashkovskaya et al, 2010). Given the phylogenetic inference and the apparent absence of chemotaxonomic properties that permit the novel strain to be distinguished from established members of the genus Bizionia (Nedashkovskaya et al, 2005(Nedashkovskaya et al, , 2010Bowman & Nichols, 2005;Bercovich et al, 2008), it seems appropriate to classify strain T-y7…”

Draft Genome Sequence of Bizionia argentinensis, Isolated from Antarctic Surface Water

“…In addition, the bacterial genome presented genes involved in the biosynthesis of carotenoids and xanthophylls. In particular, a copy of a ␤-carotene hydroxylase that catalyzes the production of ␤-cryptoxanthin and zeaxanthin, pigments detected previously in other Antarctic Flavobacteria (5) but not in members of the genus Bizionia, was found (3,8,9). According to the permanent low ambient temperature, the genome also presented a set of genes coding for the biosynthesis of unsaturated fatty acids.…”