2002
DOI: 10.1124/jpet.302.1.80
|View full text |Cite
|
Sign up to set email alerts
|

Bisphenol A Inhibits Cl Secretion by Inhibition of Basolateral K+ Conductance in Human Airway Epithelial Cells

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
7
0

Year Published

2004
2004
2016
2016

Publication Types

Select...
6
2

Relationship

2
6

Authors

Journals

citations
Cited by 14 publications
(7 citation statements)
references
References 38 publications
(51 reference statements)
0
7
0
Order By: Relevance
“…For this measurement, the basolateral membrane was permeabilized with the pore-forming antibiotic nystatin (100 M) for more than 30 min. This procedure avoids the complexities associated with basolateral ion transporters and permits analyses of apical membrane Cl Ϫ conductance (12,15). Complete permeabilization of the basolateral membrane with 100 M nystatin was preliminarily confirmed by basolateral application of bumetanide, an inhibitor of the Na ϩ -K ϩ -2Cl Ϫ cotransporter located on the basolateral membrane of Calu-3 cells, and a bumetanide-sensitive component was not detected 30 min after addition of nystatin (data not shown).…”
Section: Measurement Of Apical CL ϫ Current and Basolateral K ؉ Currentmentioning
confidence: 88%
See 1 more Smart Citation
“…For this measurement, the basolateral membrane was permeabilized with the pore-forming antibiotic nystatin (100 M) for more than 30 min. This procedure avoids the complexities associated with basolateral ion transporters and permits analyses of apical membrane Cl Ϫ conductance (12,15). Complete permeabilization of the basolateral membrane with 100 M nystatin was preliminarily confirmed by basolateral application of bumetanide, an inhibitor of the Na ϩ -K ϩ -2Cl Ϫ cotransporter located on the basolateral membrane of Calu-3 cells, and a bumetanide-sensitive component was not detected 30 min after addition of nystatin (data not shown).…”
Section: Measurement Of Apical CL ϫ Current and Basolateral K ؉ Currentmentioning
confidence: 88%
“…In this basolateral solution, CaCl 2 was increased to 4 mM to compensate for the Ca 2ϩ -chelating capacity of the gluconate (17). On the other hand, the K ϩ current across the basolateral membrane (I K ) was estimated after permeabilization of the apical membrane with nystatin (50 M) for more than 30 min and establishment of an apical-to-basolateral K ϩ concentration gradient (12,15). Complete permeabilization of the apical membrane with 50 M nystatin had been confirmed by apical application of phlorizin, an inhibitor of the Na ϩ -glucose transporter located on the apical membrane of this cell line (18).…”
Section: Measurement Of Apical CL ϫ Current and Basolateral K ؉ Currentmentioning
confidence: 99%
“…To investigate apical membrane Cl 2 conductance, apical membrane Cl 2 currents (aI Cl ) in the apical-to-basolateral Cl 2 concentration gradient were estimated after permeabilization of the basolateral membrane with nystatin (100 mM) for more than 20 minutes, as previously described (17)(18)(19). The aI Cl reflects Cl 2 permeability through CFTR in Calu-3 cells (18,20).…”
Section: Bioelectric Measurementsmentioning
confidence: 99%
“…Similarly, we found that CPZ attenuated adenosine or NECA-induced I sc by ϳ30% (data not shown). However, CPZ also blocks various membrane receptors and ion channels and reportedly inhibits basolateral K Ca channels in Calu-3 cells (21). This direct effect on basolateral K Ca channels, also observed by us in Ussing chamber and singlechannel studies (data not shown), compromises the utility of CPZ as a tool for determining whether adenosine activates potassium channels by PLA2 signaling.…”
Section: Might Adenosine Activate Other Basolateral Potassium Channels?mentioning
confidence: 71%
“…Clotrimazole (20 M), a commonly used blocker of K Ca channels (10,13,21,46), reduced the ⌬I sc induced by 10 M mucosal adenosine by ϳ47% (Fig. 2, A and B).…”
Section: Adenosine-induced I Sc Was Dependent On Neither Pkc Nor Crosmentioning
confidence: 97%