Apical adenosine regulates basolateral Ca²⁺-activated potassium channels in human airway Calu-3 epithelial cells

Abstract

Apical adenosine regulates basolateral Ca 2ϩ -activated potassium channels in human airway Calu-3 epithelial cells.

Epinephrine stimulation of anion secretion in the Calu-3 serous cell model

“…1). The cyclic nature of the response has been described previously in response to effectors that increase intracellular cAMP (21,35,38). However, it is important to note that this type of response is not uniformly described in publications examining compounds that stimulate ion transport in Calu-3 cells.…”

“…Cells grown to confluent monolayers were used on the 14th-16th day after being seeded onto the Transwells, the time at which the cells show a high-resistance phenotype. As previously reported, there is a batch-to-batch variability in the cells (7,16,38); therefore, in all experimental protocols, control and treated cultures from the same seeding were analyzed in parallel. The average transepithelial resistance for each batch of cells varied between 500 and 700 ⍀/cm 2 .…”

Keratin K18 Increases Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Surface Expression by Binding to Its C-terminal Hydrophobic Patch

“…Cell Culture, Transfection, and Viral Gene Transfers-Human airway epithelial Calu-3 cells were grown as described previously on plastic dishes or permeable supports (24). HEK293T and COS-7 cells were grown in Dulbecco's modified Eagle's medium (Invitrogen) supplemented with 10% fetal bovine serum in a 5% CO 2 , 95% air incubator at 37°C.…”

“…For short circuit current (I sc ) measurement, the procedures were essentially the same as described previously (24). Calu-3 epithelial monolayers infected with lentiviruses were grown on Snapwells to confluency (air interface culture; resistance Ͼ 800 ohms⅐cm 2 ) and then mounted in Ussing chambers for experiments.…”

Quantum dots modulate intracellular Ca²⁺ level in lung epithelial cells

“…11 It has been further shown that the polarity of a cell monolayer can be changed by ion transport, fluid secretion, and ciliary movement. 12 Abnormality in Ca 2+ homeostasis in the airway epithelium may, therefore, contribute to development of airway hyper-responsiveness and the exacerbation of chronic lung diseases, such as asthma, COPD, silicosis/fibrosis, and lung infections.…”