Bisphenol A Exacerbates Allergic Inflammation in an Ovalbumin-Induced Mouse Model of Allergic Rhinitis

Abstract

Purpose. Bisphenol A (BPA) is found in many plastic products and is thus a common environmental endocrine disruptor. Plastic-related health problems, including allergic diseases, are attracting increasing attention. However, few experimental studies have explored the effect of BPA on allergic rhinitis (AR). We explore whether BPA was directly related to the allergic inflammation induced by ovalbumin (OVA) in AR mice. Methods. We first constructed OVA-induced mouse model, and after BPA administration, we evaluated nasal symptoms and measured the serum OVA-specific IgE levels by ELISA. Th2 and Treg-related cytokines of nasal mucosa were measured by cytometric bead array. Th2 and Treg-specific transcription factor levels were assayed by PCR. The proportions of CD3+CD4+IL-4+Th2 and CD4+Helios+Foxp3+ T cells (Tregs) in spleen tissue were determined by flow cytometry. Results. Compared to OVA-only-induced mice, BPA addition increased nasal symptoms and serum OVA-specific IgE levels. OVA and BPA coexposure significantly increased IL-4 and IL-13 protein levels compared to those after OVA exposure alone. BPA plus OVA tended to decrease the IL-10 protein levels compared to those after OVA alone. Coexposure to OVA and BPA significantly increased the GATA-3-encoding mRNA level, and decreased the levels of mRNAs encoding Foxp3 and Helios, compared to those after OVA exposure alone. BPA increased the Th2 cell proportion, and decreased that of Tregs, compared to the levels with OVA alone. Conclusion. BPA exerted negative effects by exacerbating AR allergic symptoms, increasing serum OVA-specific IgE levels, and compromising Th2 and Treg responses.

Ajwain oil attenuates allergic response of ovalbumin‐induced allergic rhinitis via alteration of inflammatory, oxidative stress, and Th1/Th2 responses

“…IgE is the significant biomarker of allergic diseases including allergic asthma and AR. IgE can bind with the high affinity IgE receptor on the surface of mast cells and basophils to sensitize them and start the secretion of inflammatory mediators such as leukotriene and histamine when exposed to the same allergen again (Kim et al., 2018; Li et al., 2018; Wang et al., 2020). IgE is a significant biomarker to scrutinize the efficiency of anti‐allergic drugs and scrutinize the possible mechanism.…”

“…Moreover, IL‐4, a significant cytokine of up‐regulating IgE, IFN‐γ is an anti‐allergic factor of respiratory tract and biological antagonist of IL‐4 synthesis. In the development and activation of eosinophils, IL‐5 plays a critical function, increased survival time and is a significant marker in the delayed phase of hypersensitivity (Chen, Chan, et al., 2020; Piao et al., 2020; Wang et al., 2020). Airway hyper responsiveness requires IL‐5‐mediated eosinophil infiltration in the trachea (Chen, Chan, et al., 2020; Piao et al., 2019).…”

“…These treatments can reduce the inflammatory response system. Although AR is not a life‐threatening condition, clinical symptoms such as itching, rhinorrhea, sneezing, and nasal congestion make life more difficult and add to the financial burden (Wang et al., 2020; Yang et al., 2021; Yuan et al., 2019). AR affected the various organs via induction and alteration of edema.…”

A New Experimental Allergic Rhinitis Model in Mice

“…AR has been induced in mice after IP injections of OVA on days 0, 7 and 14, followed by its intranasal applications twice a day for three weeks (8,17) . Bisphenol A (BPA) has been reported to exacerbate allergic inflammation in an ovalbumin-dependent AR mouse model (18) . In this study, BPA was given to mice after they received ovalbumin IP on days 0, 7 and 14. it was reported that allergic symptoms of AR were exacerbated and serum levels of OVA-specific IgE levels increased in mice (18) .…”

“…Bisphenol A (BPA) has been reported to exacerbate allergic inflammation in an ovalbumin-dependent AR mouse model (18) . In this study, BPA was given to mice after they received ovalbumin IP on days 0, 7 and 14. it was reported that allergic symptoms of AR were exacerbated and serum levels of OVA-specific IgE levels increased in mice (18) . In a study that investigating the effect of mesenchymal stem cells of human umbilical cord in AR treatment, IP ovalbumin was given to mice on the 0,7, and 14 th days to induce AR (19) .…”