Bisphenol A degradation in water by ligninolytic enzymes

Gassara, Fatma; Brar, Satinder Kaur; Verma, Mausam P.; Tyagi, Rajeshwar Dayal

doi:10.1016/j.chemosphere.2013.02.071

Cited by 81 publications

(36 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A low value of K m indicates a very strong affinity, which means that the maximum rate is reached at a relatively low substrate concentration. Gassara et al reported K m values of 10.41, 13.33, 15.63, 1.10 and corresponding V m values of 0.04, 1.12, 1.16, 1.04 (mg L −1 h −1 ) for BPA treatment by using free, pectin encapsulated, carboxy methyl cellulose encapsulated, and gelatine encapsulated ligninolytic enzymes, respectively . A high BPA degradation velocity with a moderate K m value was obtained in this study by using F. trogii crude extract.…”

Section: Resultssupporting

confidence: 57%

“…The Michaelis-Menten and Lineweaver-Burk plots between rates, V and BPA concentration S are given in Fig. 4 [13]. A high BPA degradation velocity with a moderate K m value was obtained in this study by using F. trogii crude extract.…”

Section: Initial Bpa Concentration and Kinetic Studiessupporting

confidence: 53%

“…The pure enzyme could not reduce the pollutants concentration effectively [12]. Lower BPA degradation was reported with free enzymes than encapsulated enzymes [13].…”

Section: Introductionmentioning

confidence: 93%

See 2 more Smart Citations

Biodegradation and Detoxification of BPA: Involving Laccase and a Mediator

Erkurt

2015

CLEAN Soil Air Water

View full text Add to dashboard Cite

Funalia trogii was used for the first time for the removal of bisphenol A (BPA), a well known endocrine disrupting compound. Biodegradation efficiencies of commercial pure laccase from Trametes versicolor, crude extract of F. trogii and the mixture of pure laccase from T. versicolor, and thermally inactivated crude extract of F. trogii were compared. BPA was completely removed by both the crude extract and with mixture whereas only 30% was removed with pure laccase. The results showed the presence of mediator molecules in the crude extract of F. trogii and also confirm the role of laccase in BPA biodegradation. A mediator molecule, butylhydroxytoluene, was detected by GC‐MS analysis of the crude extract of F. trogii. The results also proved unnecessity of enzyme isolation procedures for the enzymatic biodegradation of BPA. The BPA concentration was analyzed by HPLC and metabolites of BPA were characterized by GC‐MS. The results of both analyses were correlated and BPA was completely removed after 2 h of incubation time. The reaction maximum velocity and Km values obtained from Lineweaver–Burk plots of Michaelis–Menten equation were 7.43 mg L−1 min−1 and 66.35 mg/L, respectively. The acute toxicity of BPA and its products after 6 h incubation period was 98.9 ± 1.0 and 7.5 ± 0.4%, respectively. The results of this study point out the use of an alternative feasible method for the complete removal of BPA from polluted environments.

show abstract

Section: Resultssupporting

confidence: 57%

Section: Initial Bpa Concentration and Kinetic Studiessupporting

confidence: 53%

See 1 more Smart Citation

Biodegradation and Detoxification of BPA: Involving Laccase and a Mediator

Erkurt

2015

CLEAN Soil Air Water

View full text Add to dashboard Cite

show abstract

“…A careful comparison of results present in the recent scientific literature reveals that different strategies have been used to obtain BPA removal, along with different time of reaction and concentration of both enzyme and substrate. Gassara and coworkers [26] reported a rate of BPA degradation of 13% after 2 hours of incubation in the presence of 0.05 U/mL of a laccase from Phanerochaete chrysosporium . A purified laccase from Grifola frondosa was able to degrade 15% BPA (0.65 mM) in 1 hour [27], whereas a purified laccase from Phlebia tremellosa [28] removed around 65% of BPA estrogenic activity after 3 h incubation with 50 U of enzymatic activity.…”

Section: Resultsmentioning

confidence: 99%

Fungal Laccases Degradation of Endocrine Disrupting Compounds

Macellaro

Pezzella

Cicatiello

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

Over the past decades, water pollution by trace organic compounds (ng/L) has become one of the key environmental issues in developed countries. This is the case of the emerging contaminants called endocrine disrupting compounds (EDCs). EDCs are a new class of environmental pollutants able to mimic or antagonize the effects of endogenous hormones, and are recently drawing scientific and public attention. Their widespread presence in the environment solicits the need of their removal from the contaminated sites. One promising approach to face this challenge consists in the use of enzymatic systems able to react with these molecules. Among the possible enzymes, oxidative enzymes are attracting increasing attention because of their versatility, the possibility to produce them on large scale, and to modify their properties. In this study five different EDCs were treated with four different fungal laccases, also in the presence of both synthetic and natural mediators. Mediators significantly increased the efficiency of the enzymatic treatment, promoting the degradation of substrates recalcitrant to laccase oxidation. The laccase showing the best performances was chosen to further investigate its oxidative capabilities against micropollutant mixtures. Improvement of enzyme performances in nonylphenol degradation rate was achieved through immobilization on glass beads.

show abstract

“…Library search has identified multiple products with shorter retention time than BPA (Table 1). Some of those compounds have been previously reported as BPA degradation products such as carboxylic or polycarboxylic acids and β-hydroxybutyric acid (Gassara et al 2013;Daassi et al 2016). These findings are confirmed by Husain et al (2013) who reported the accumulation of acidic intermediates after biological and enzymatic BPA treatments.…”

Section: Bpa Degradation Metabolites Analysismentioning

confidence: 97%

Biodegradation and detoxification of bisphenol A by bacteria isolated from desert soils

et al. 2019

View full text Add to dashboard Cite

The endocrine-disrupting chemical bisphenol A (BPA) has attracted much attention because of its estrogenic activity and widespread environmental contamination. In this study, we investigated the BPA biodegradation abilities of various bacterial strains isolated from deserts and arid soils from southern Tunisia. Ten bacterial strains that belong to Pseudomonas putida, Pseudomonas aeruginosa, Enterobacter cloacae, Klebsiella sp. and Pantoea sp. showed high BPA removal potential in mineral salt medium (MSM) containing 1 mM BPA. BPA removal rates varied between strains and ranged from 36 to 97%. The strain G320 (P. putida) presented the highest BPA removal rate with 97% within 4 days at 30 °C. The half-life when increasing the BPA concentration to 3 mM was 2 days for strain G320, while total degradation was achieved within 8 days. BPA biodegradation products were identified by GC-MS, and their toxicity was assessed by an algal toxicity test. BPA detoxification was confirmed by evaluating the effect of its biodegradation metabolites on algal growth (dry weight), cells morphology and chlorophylls levels of Tetraselmis sp. strain V2. Results showed the interesting potential of desert soil's bacteria in BPA detoxification as well as the eventual use of the algal specie in toxicity assessment.

show abstract

Bisphenol A degradation in water by ligninolytic enzymes

Cited by 81 publications

References 16 publications

Biodegradation and Detoxification of BPA: Involving Laccase and a Mediator

Biodegradation and Detoxification of BPA: Involving Laccase and a Mediator

Fungal Laccases Degradation of Endocrine Disrupting Compounds

Biodegradation and detoxification of bisphenol A by bacteria isolated from desert soils

Contact Info

Product

Resources

About