Fungal Laccases Degradation of Endocrine Disrupting Compounds

Abstract

Over the past decades, water pollution by trace organic compounds (ng/L) has become one of the key environmental issues in developed countries. This is the case of the emerging contaminants called endocrine disrupting compounds (EDCs). EDCs are a new class of environmental pollutants able to mimic or antagonize the effects of endogenous hormones, and are recently drawing scientific and public attention. Their widespread presence in the environment solicits the need of their removal from the contaminated sites. One promising approach to face this challenge consists in the use of enzymatic systems able to react with these molecules. Among the possible enzymes, oxidative enzymes are attracting increasing attention because of their versatility, the possibility to produce them on large scale, and to modify their properties. In this study five different EDCs were treated with four different fungal laccases, also in the presence of both synthetic and natural mediators. Mediators significantly increased the efficiency of the enzymatic treatment, promoting the degradation of substrates recalcitrant to laccase oxidation. The laccase showing the best performances was chosen to further investigate its oxidative capabilities against micropollutant mixtures. Improvement of enzyme performances in nonylphenol degradation rate was achieved through immobilization on glass beads.

Expression of a new laccase from Moniliophthora roreri at high levels in Pichia pastoris and its potential application in micropollutant degradation

“…Several reports describe effective removal of EDCs and NSAIDs by other laccases as well. Those experiments were carried out at acidic pH values, due to higher laccase activities at these pH values (Asadgol et al 2014; Garcia-Morales et al 2015; Macellaro et al 2014; Sei et al 2008; Tsutsumi et al 2001). However, wastewater of plant effluents, which are considered the main source of estrogens (Snyder et al 2001), usually show a neutral or basic pH.…”

Exploitation of Trametes versicolor for bioremediation of endocrine disrupting chemicals in bioreactors

“…Then, 200 μL of obtained sample was analysed using a C18 column as previously described [18]. A concentration of 0.1 μM corresponds to the limit of detection of the EDCs in the applied conditions.…”

Solid State Fermentation: Comprehensive Tool for Utilization of Lignocellulosic through Biotechnology

“…Curvularia lunata Phanerochaete chrysosporium [189] Polymeric model dye Poly R-478 Manganese peroxidase Irpex lacteus [141] Nonylphenol Laccase P. ostreatus [190] 2,4-dinitrophenol Laccase T. versicolor [191] Phenol Laccase P. simplicissimum [192] Naphthalene, Anthracene and Benzo[a]anthracene Laccase Lentinula edodes [193] Fluorene Laccase Coprinus plicatilis [194] Malachite green Laccase Bacillus thuringiensis [195] Bisphenol A Laccase Funalia trogii [196] Anthroquinone Laccase Lentinus sp [197] Salicylic acid, Naproxen, Ibuprofen, Gemfibrozil, Diclofenac and Triclosan Laccase Trametes versicolor [198] Bisphenol A and Diclofenac Laccase Aspergillus oryzae. [199] Endocrine Disrupters Laccase Cerrena unicolor [200] Textile effluent Laccase Pleurotus ostreatus IBL-02 and Coriolus versicolor [201] Dyes Peroxidase P.ostreatus [202] Olive [208] Methylene blue Mangenese peroxidase Phanerochaete chrysosporium [209] Versatile peroxidase and laccase Pleurotus ostreatus [210] coracryl brilliant blue, Ligninolytic enzymes Phanerochaete chrysosporium, Phlebia brevispora and Phlebia floridensis [211] graphene Lignin peroxidase White rot fungi [212] Sweet Sorghum Bagasse…”