Bisdemethoxycurcumin induces DNA damage and inhibits DNA repair associated protein expressions in NCI-H460 human lung cancer cells

Abstract

Nonsmall cell lung carcinoma (NSCLC) is a devastating primary lung tumor resistant to conventional therapies. Bisdemethoxycurcumin (BDMC) is one of curcumin derivate from Turmeric and has been shown to induce NSCLC cell death. Although there is one report to show BDMC induced DNA double strand breaks, however, no available information to show BDMC induced DNA damage action with inhibited DNA repair protein in lung cancer cells in detail. In this study, we tested BDMC-induced DNA damage and condensation in NCI-H460 cells by using Comet assay and DAPI staining examinations, respectively and we found BDMC induced DNA damage and condension. Western blotting was used to examine the effects of BDMC on protein expression associated with DNA damage and repair and results indicated that BDMC suppressed the protein levels associated with DNA damage and repair, such as 14-3-3σ (an important checkpoint keeper of DDR), O6-methylguanine-DNA methyltransferase, DNA repair proteins breast cancer 1, early onset, mediator of DNA damage checkpoint 1 but activate phosphorylated p53 and p-H2A.X (phospho Ser140) in NCI-H460 cells. Confocal laser systems microscopy was used for examining the protein translocation and results show that BDMC increased the translocation of p-p53 and p-H2A.X (phospho Ser140) from cytosol to nuclei in NCI-H460 cells. In conclusion, BDMC induced DNA damage and condension and affect DNA repair proteins in NCI-H460 cells in vitro. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1859-1868, 2016.

Chrysin inhibit human melanoma A375.S2 cell migration and invasion via affecting MAPK signaling and NF‐κB signaling pathway in vitro

“…Cell nucleus was stained with PI (red fluorescence). Leica TCS SP2 Confocal Spectral Microscope was used to examine and photomicrography as described previously . Co‐localization of cytochrome c or AIF in nuclei or cytosol was formed yellow color based on red and green overlapped pixels.…”

18α-Glycyrrhetinic Acid Induces Apoptosis of HL-60 Human Leukemia Cells through Caspases- and Mitochondria-Dependent Signaling Pathways

“…Finally, we add the DAPI solution (1 μg/mL) at 37 °C without light. Nuclear morphologies were photographed using a fluorescence microscope (Eclipse TE 300, Nikon, Tokyo, Japan) as described previously [45].…”

“…Cells were placed in 6-well plate at a density of 2 × 10 5 cells/well and cells were incubated with 18α-GA ranging from 40–120 μM for 48 h. After treatment, cells from each treatment were harvested to examine the DNA damage with the Comet assay as described previously [45].…”

Promising anti‐tumor properties of bisdemethoxycurcumin: A naturally occurring curcumin analogue

“…(1) is decreased and DNA damage is increased after BDMC (35 μM) treatment in a timedependent manner. Moreover, the proteins levels of 14-3-3r, MGMT, BRCA1, and MDC1 were found to be decreased in human lung cancer NCI-H460 cells following treatment with BDMC while the levels of p-p53 and p-H2A.X were increased (Yu, Yang, Huang et al, 2015).…”

“…Yu et al explored the impact of BDMC treatment on DNA damage and condensation in NCI‐H460 cells and found that viable cell count (1) is decreased and DNA damage is increased after BDMC (35 μM) treatment in a time‐dependent manner. Moreover, the proteins levels of 14‐3‐3r, MGMT, BRCA1, and MDC1 were found to be decreased in human lung cancer NCI‐H460 cells following treatment with BDMC while the levels of p‐p53 and p‐H2A.X were increased (Yu, Yang, Huang et al, ). S‐phase is the part of the cell cycle in which DNA is replicated, occurring between G1 and G2 phase.…”