Bis(monoacylglycerin)phosphorsäure — ein Marker-Lipid sekundärer Lysosomen?

Abstract

Zusammenfassung: Nach Applikation von Triton, Dextran, kolloidalem Silber und Eisen an Ratten wurden Lysosomenfraktionen aus den Lebern isoliert. In allen daraus gewonnen Phospholipidgemischen konnte (MAG) 2 P in Mengen von 6-28% des Gesamtlipidphosphors aufgefunden werden.Die Fettsäuregemische dieses sauren Glycerinphospholipids zeichnen sich durch einen ganz außergewöhnlich hohen Anteil von Polyensäuren (58-82% der Gesamtfettsäuren) aus, deren Haüptkomponente Docosahexaensäure ist. Bis(monoacylglycerojphosphate -a Marker Lipid of Secondary Lysosomes?Summary: Rats were treated with triton, dextran, silver and iron. The lysosomal fractions from their livers were isolated and the lipids analysed. (MAG) 2 P could be detected in every phospholipid fraction, accounting for 6 -28 per cent of the total lipid phosphorus.The composition of the fatty acids from this acidic glycerophospholipid shows an extraordinarily high amount of polyenoic fatty acids (58-82 per cent of the total), of which docosahexaenoic acid is a main compound.

Bis(monoacylglycero)phosphate: a secondary storage lipid in the gangliosidoses

“…In alveolar macrophages, BMP predominantly contains n-6 fatty acids, such as linoleic acid (18:2) and arachidonic acid (20:4) ( 75,76 ). Interestingly, DHA (C22:6, n-3) comprises a signifi cant portion of BMP fatty acids in many cell types in drug-induced phospholipidosis, and also in many LSDs ( 22,(70)(71)(72)(73)(77)(78)(79)(80)(81)(82). We also found that DHA was a major fatty acid species in BMP from the brains of mice, cats, and bears with gangliosidosis.…”

Iron uptake by rat duodenal microvillous membrane vesicles: evidence for a carrier mediated transport system

“…All operations were performed at 4°C. Microvillous membrane vesicle preparations were characterized by electron microscopy [15] and enzymatic markers, employing sucrase (EC 3·2·1·48) [16] as marker for MVM; Na+/ K + -ATPase (EC 3·6·1·3) [17] as a constituent of the basolatera1 membranes; glucose-6-phosphatase (EC 3·1·3·9·1 [18] as a marker for microsomal contamination; succinate dehydrogenase (EC 1-3-99,1) [19] for mitochondria; acid phosphatase (EC 3·1·3·2) [20] for secondary 1ysosomes and galactosyltransferase (EC 2·4·1·38) [21] for golgi contamination. Membrane preparations were analysed by double immunodiffusion in agar [22] against antibodies to rat-ligandin [23], rat-transferrin and to whole rat serum proteins (all from Cappel Laboratories, Cochranville, PA) to detect cytoplasmatic and serum protein contamination…”

Pulmonary and Generalized Lysosomal Storage Induced by Amphiphilic Drugs

“…BMGP contains very high amounts of polyenoic fatty acids, particularly docasahexaenoic acid (167,168). It is synthesized in lysosomes (169,170) by the BMGP synthetase by transfer of acyl groups from phosphatidylinositol to phosphatidylglycerol or lysophosphatidylglycerol (76).…”

“…It is a normal component of lysosomal PLs, where it may account for 6 to 28% of total PLs (76,167,171). Some of the lysosomal BMGP could have its origin in the plasma membrane (167).…”