Birth of healthy calves after intra-follicular transfer (IFOT) of slaughterhouse derived immature bovine oocytes

Hoelker, M.; Kassens, Ana; Salilew‐Wondim, Dessie; Sieme, Harald; Wrenzycki, C.; Tesfaye, Dawit; Neuhoff, Christiane; Schellander, K.; Held-Hoelker, E.

doi:10.1016/j.theriogenology.2017.04.009

Cited by 18 publications

(30 citation statements)

References 47 publications

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“…The average number of embryos obtained was 10.8%. This figure does not differ much from the data in the available literature where 8.4%, 5.5%, and 17.3% of the recovered embryos are reported (Kassens et al 2015;Sprícigo et al 2016;Hoelker et al 2017). However, our results come from a low number of sessions, and therefore the best result achieved by endoscopic fallopian tube collection has certainly positively distorted the overall average.…”

Section: Discussioncontrasting

confidence: 61%

“…The numbers and quality of recovered objects (embryos/oocytes) collected from the uterus 7 days after IFOT were very variable. In total, 23.1% of the objects were obtained, which is slightly less than in the available literature, where 35.2%, 21.3%, and 38.5% were reported (Kassens et al 2015;Sprícigo et al 2016;Hoelker et al 2017). The average number of embryos obtained was 10.8%.…”

Section: Discussionmentioning

confidence: 66%

“…In the following study, immature oocytes were transferred into growing follicles prior to ovulation. This study demonstrated that even in vitro maturation can be bypassed by direct transfer of collection cumulus-oocytes complexes into the follicle (Hoelker et al 2017). Intrafollicular oocyte transfer using immature fresh or vitrified oocytes collected from living donors using transvaginal aspiration was reported (Sprícigo et al 2016).…”

mentioning

confidence: 87%

“…Since oocyte collection and oocyte transfer do not differ substantially, OPU systems have been slightly modified in order to perform first studies on IFOT. Equipment described in all mentioned IFOT studies (Kassens et al 2015;Sprícigo et al 2016;Hoelker et al 2017) consists of a plastic holder and an ultrasound transducer (7.5-10 MHz). A long tubing system was connected with a disposable needle (G26, G27) at one end and with either a laboratory pipette or a 1 ml syringe at the other end.…”

mentioning

confidence: 99%

“…The tubing system was filled with fluid (phosphate-buffered saline or follicular fluid). Oocytes were loaded into the needle at a fluid volume of 60 or 200 µl (Kassens et al 2015;Sprícigo et al 2016;Hoelker et al 2017). Handling required at least two people: one person performed rectal manipulation and navigated the follicle bearing ovary in front of the ultrasound; the second person introduced IFOT set and took care of the careful oocyte transfer into the follicle.…”

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confidence: 99%

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Intrafollicular oocyte transfer in cattle – a technical report

et al. 2020

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The aim of the study was to develop and evaluate the functionality of a new equipment for intrafollicular oocyte transfer (IFOT) in dairy cattle. The new system for IFOT is composed of the applicator, the aspirator, and the injector. After aspiration of oocytes, the IFOT set is inserted into the working tube of the ultrasound transducer holder, the content of the applicator can be injected into the preovulatory follicle via transvaginal ultrasonography by one operator. The function of instruments used for IFOT was firstly verified in laboratory conditions. Slaughterhouse oocytes filled into the instruments were injected into Petri dishes. The highest recovery rates in vitro (97.5%) were achieved when the applicator was stored with the needle in a downward position. Synchronized Holstein heifers were used for in vivo test. Intrafollicular injection of saline (n = 9) was performed to find whether ovulation is affected by the injection. Then IFOTs of phosphate buffered saline with 20 oocytes (n = 21) were performed into the preovulatory follicles followed by 7-day-old embryo collection. Total ovulation rates were 86.7% (26/30). Total recovery rates (oocytes + embryos) were 23.1%, embryo recovery rates were 10.1%. The new instrument allows for the loading of oocytes and easy transportation to recipients, and also allows IFOT to be performed by one person in field conditions. The method does, however, need further investigation.

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Section: Discussioncontrasting

confidence: 61%

Section: Discussionmentioning

confidence: 66%

mentioning

confidence: 87%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Intrafollicular oocyte transfer in cattle – a technical report

et al. 2020

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Birth of first twin calves produced by intrafollicular transfer of immature oocyte

Trevisol,

Melo,

Lera

et al. 2023

Vet Record Case Reports

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In an adequate system of nutrition and management, the birth of twin calves can increase the productive efficiency in beef cattle. In this sense, genetic selection, hormonal treatment and the use of embryo transfer are strategies to produce twins in cattle. In the present report, we describe an alternative method for the production of twins based on the intrafollicular transfer of immature oocytes that resulted in the birth of the first twins reported using this technique. The expectation is to significantly improve the performance of the technique in the near future, making it another reproductive tool to be applied in the production and research sectors.

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In-vitro-Produktion von Nutztier-Embryonen

Wrenzycki

2023

Biotechnologie Bei Nutztieren 1

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Birth of healthy calves after intra-follicular transfer (IFOT) of slaughterhouse derived immature bovine oocytes

Cited by 18 publications

References 47 publications

Intrafollicular oocyte transfer in cattle – a technical report

Intrafollicular oocyte transfer in cattle – a technical report

Birth of first twin calves produced by intrafollicular transfer of immature oocyte

In-vitro-Produktion von Nutztier-Embryonen

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