Birefringence measurement of biological tissue based on polarization-sensitive digital holographic microscopy

Wang, Jiawen; Dong, Liang; Chen, Haige; Huang, Sujuan

doi:10.1007/s00340-018-7098-3

Cited by 15 publications

(11 citation statements)

References 28 publications

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“…However, this technique has started to be used for biological imaging only in the last few years; therefore, the scientific papers on this field of application present in the literature are still a low number. For example, Wang et al [67] studied the birefringence distribution of biological tissues by using polarization-dependent phase-shifted holograms. Even if the authors used a setup based on a modified Mach-Zehnder interferometer, which allows the recording polarization holograms by rotating a polarizer and so requiring multiple acquisitions, this approach takes advantage by DH and, thus, can be exploited to have only one image acquisition.…”

Section: Biologicalmentioning

confidence: 99%

Polarization-Sensitive Digital Holographic Imaging for Characterization of Microscopic Samples: Recent Advances and Perspectives

Coppola¹,

Ferrara²

2020

Applied Sciences

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Polarization-sensitive digital holographic imaging (PS-DHI) is a recent imaging technique based on interference among several polarized optical beams. PS-DHI allows simultaneous quantitative three-dimensional reconstruction and quantitative evaluation of polarization properties of a given sample with micrometer scale resolution. Since this technique is very fast and does not require labels/markers, it finds application in several fields, from biology to microelectronics and micro-photonics. In this paper, a comprehensive review of the state-of-the-art of PS-DHI techniques, the theoretical principles, and important applications are reported.

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Section: Biologicalmentioning

confidence: 99%

Polarization-Sensitive Digital Holographic Imaging for Characterization of Microscopic Samples: Recent Advances and Perspectives

Coppola¹,

Ferrara²

2020

Applied Sciences

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“…Ultimately, PS imaging systems are advantageous in biological imaging increasing the understanding of cell biological processes [21][22][23][24] since they determine the isotropic properties of different cellular and extracellular molecular components, such as microtubules and amyloid. 21 The aim of this work is twofold. First, we demonstrate the advantages of QPI imaging using a Fresnel biprism (FB)-based DHM system.…”

Section: Introductionmentioning

confidence: 99%

“…Ultimately, PS imaging systems are advantageous in biological imaging increasing the understanding of cell biological processes 21 – 24 since they determine the isotropic properties of different cellular and extracellular molecular components, such as microtubules and amyloid. 21 …”

Section: Introductionmentioning

confidence: 99%

Advantages of Fresnel biprism-based digital holographic microscopy in quantitative phase imaging

et al. 2020

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Significance: The hallmarks of digital holographic microscopy (DHM) compared with other quantitative phase imaging (QPI) methods are high speed, accuracy, spatial resolution, temporal stability, and polarization-sensitivity (PS) capability. The above features make DHM suitable for real-time quantitative PS phase imaging in a broad number of biological applications aimed at understanding cell growth and dynamic changes occurring during physiological processes and/or in response to pharmaceutical agents. Aim: The insertion of a Fresnel biprism (FB) in the image space of a light microscope potentially turns any commercial system into a DHM system enabling QPI with the five desired features in QPI simultaneously: high temporal sensitivity, high speed, high accuracy, high spatial resolution, and PS. To the best of our knowledge, this is the first FB-based DHM system providing these five features all together. Approach: The performance of the proposed system was calibrated with a benchmark phase object. The PS capability has been verified by imaging human U87 glioblastoma cells. Results: The proposed FB-based DHM system provides accurate phase images with high spatial resolution. The temporal stability of our system is in the order of a few nanometers, enabling live-cell studies. Finally, the distinctive behavior of the cells at different polarization angles (e.g., PS capability) can be observed with our system. Conclusions: We have presented a method to turn any commercial light microscope with monochromatic illumination into a PS QPI system. The proposed system provides accurate quantitative PS phase images in a new, simple, compact, and cost-effective format, thanks to the low cost (a few hundred dollars) involved in implementing this simple architecture, enabling the use of this QPI technique accessible to most laboratories with standard light microscopes.

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“…Because the tested sample will be magnified through the MO, the resolution of the reconstructed information would reach a sub-micrometer level. DHM technique has been applied successfully in some fields such as Microelectromechanical Systems (MEMS) device morphology, deformation detection [ 6 , 7 ], workpiece surface roughness detection [ 8 , 9 ], non-preparation detection of living cells or biological tissue [ 10 , 11 ], transparent functionally gradient material detection [ 12 ], etc.…”

Section: Introductionmentioning

confidence: 99%

Tip Crack Imaging on Transparent Materials by Digital Holographic Microscopy

Zhou

Shen

et al. 2019

J. Imaging

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With this study, we propose a method to image the tip crack on transparent materials by using digital holographic microscopy. More specifically, an optical system based on Mach–Zehnder interference along with an inverted microscopy (Olympus CKX53) was used to image the tip crack of Dammar Varnish transparent material under thermal excitation. A series of holograms were captured and reconstructed for the observation of the changes of the tip crack. The reconstructed holograms were also compared temporally to compute the temporal changes, showing the crack propagation phenomena. Results show that the Dammar Varnish is sensitive to the ambient temperature. Our research demonstrates that digital holographic microscopy is a promising technique for the detection of the fine tip crack and propagation in transparent materials.

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Birefringence measurement of biological tissue based on polarization-sensitive digital holographic microscopy

Cited by 15 publications

References 28 publications

Polarization-Sensitive Digital Holographic Imaging for Characterization of Microscopic Samples: Recent Advances and Perspectives

Polarization-Sensitive Digital Holographic Imaging for Characterization of Microscopic Samples: Recent Advances and Perspectives

Advantages of Fresnel biprism-based digital holographic microscopy in quantitative phase imaging

Tip Crack Imaging on Transparent Materials by Digital Holographic Microscopy

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