Birch allergy and oral allergy syndrome: The practical relevance of serum immunoglobulin E to Bet v 1

Ciprandi, Giorgio; Comite, Paola; Ferrero, Francesca; Bignardi, Donatella; Minale, Paola; Voltolini, Susanna; Troise, C; Mussap, Michele

doi:10.2500/aap.2016.37.3914

Cited by 28 publications

(20 citation statements)

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“…This finding potentially indicates that sIgE levels can be a biomarker for differentiating sensitisation from real allergy as has been proposed by other authors4243. Moreover the anaphylactic group, treated with Pru p 3 + LPS, also showed a significant increase of Pru p 3 specific IgG1 that was not observed in mice sensitised with Pru p 3 only.…”

Section: Discussionsupporting

confidence: 80%

LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model

Rodríguez

Aranda

Fernández

et al. 2017

Sci Rep

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Pru p 3 is the major peach allergen in the Mediterranean area. It frequently elicits severe reactions, limiting its study in humans, raising the need for animal models to investigate the immunological mechanisms involved. However, no anaphylaxis model exists for Pru p 3. We aimed to develop a model of peach anaphylaxis by sensitising mice with Pru p 3 in combination with lipopolysaccharide (LPS) as an adjuvant. Four groups of mice were sensitised intranasally: untreated; treated with Pru p 3; treated with LPS; treated with Pru p 3 + LPS. After sensitisation mice were intraperitoneally challenged with Pru p 3 and in vivo and in vitro parameters were evaluated. Only mice in the Pru p 3 + LPS group showed anaphylaxis symptoms, including a decrease in temperature. Determination of in vitro parameters showed a Th2 response with an increase of Pru p 3-specific IgE and IgG1. Moreover, at the cellular level, we found increased levels of IgE and IgG1 secreting Pru p 3-specific cells and a proliferative CD4+ T-cell response. These results demonstrate that Pru p 3-specific anaphylaxis can be generated after nasal sensitisation to Pru p 3 in combination with LPS. This is a promising model for evaluating food allergy immunotherapies.

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Section: Discussionsupporting

confidence: 80%

LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model

Rodríguez

Aranda

Fernández

et al. 2017

Sci Rep

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“…This is in agreement with the high prevalence of birch allergy with a Bet v 1 polarized pattern (18). This Bet v 1 preponderance influences the low prevalence of oral allergy syndrome in this region (19). Bet v 1 predominance might affect grass pattern.…”

Section: Discussionsupporting

confidence: 75%

Phleum pratense molecular pattern across Italy

Ciprandi

Scala²,

Ariano³

2017

Eur Ann Allergy Clin Immunol

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“…An increased occurrence of allergic rhinitis with elevated Bet v 1-specific IgE levels or higher numbers of PR-10 molecule recognition proteins has been found in a pediatric population [29], and a strict relationship has been detected between the occurrence of OAS and higher Bet v 1 IgE levels [30,31]. Accordingly, we found higher Aln g 1, Bet v 1, Cor a 1.0101, Cor a 1.0401, Mal d 1, and Pru p 1 IgE levels in patients affected by OAS when compared with PR-10-reactive individuals without food allergy, as also suggested by previous observations with an extract-based diagnostic approach [32,33], thus demonstrating that a broader range of PR-10 detection can be useful to unveil more aggressive clinical profiles.…”

Section: Discussionmentioning

confidence: 99%

Molecular Recognition Profiles and Clinical Patterns of PR-10 Sensitization in a Birch-Free Mediterranean Area

Scala

Abeni

Cecchi³

et al. 2017

Int Arch Allergy Immunol

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Background: The order Fagales represents an important cause of tree-pollen allergy in northern countries. We investigated the IgE recognition profiles, mutual relationships, and association with clinical symptoms of a panel of allergens belonging to the PR-10 family, the main proteins responsible for Fagales allergy (Act d 8, Aln g 1, Api g 1, Ara h 8, Bet v 1, Cor a 1.0101, Cor a 1.0401, Gly m 4, Mal d 1, and Pru p 1). Methods: A total of 526 PR-10-reactive subjects living in central and southern Italy were studied by ImmunoCAP-ISAC-112 microarray analysis. Results: Overall, Bet v 1 reactivity was the most commonly (74%) observed among PR-10 proteins, but Cor a 1.0101 was the most prevalent in participants aged <6 years, and between 15 and 65 years. Overall, 26% of the PR-10-reactive persons were Bet v 1 negative, whilst 93.6% of the PR-10 polyreactive individuals were Bet v 1 positive. Among the 10 PR-10s evaluated, 100 combinations were recorded. The strongest association was observed between molecules with the highest sequence identities (Bet v 1 and Cor a 1.0101, Cor a 1.0401 or Aln g 1; Mal d 1 and Pru p 1). Bet v 1-, Cor a 1.0101-, and Aln g 1-specific IgE recognition was associated with respiratory symptoms, whilst Ara h 8, Cor a 1.0401, Gly m 4, Mal d 1, and Pru p 1 were selectively linked to an oral allergic syndrome. Conclusions: Testing IgE reactivity to a panel of PR-10s in a birch-free area discloses peculiar relationships between clinical phenotypes and sensitization profiles, allowing the identification of novel cluster patterns.

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Birch allergy and oral allergy syndrome: The practical relevance of serum immunoglobulin E to Bet v 1

Abstract: The present study demonstrated that serum IgE levels to Bet v 1 could be a useful marker for differentiating between different birch pollen sensitization phenotypes.

Cited by 28 publications

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LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model

LPS promotes Th2 dependent sensitisation leading to anaphylaxis in a Pru p 3 mouse model

Phleum pratense molecular pattern across Italy

Molecular Recognition Profiles and Clinical Patterns of PR-10 Sensitization in a Birch-Free Mediterranean Area

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