Bir Ceylanda (Gazella gazella) Generalize Reaktif Amiloidoz Olgusu

Abstract

ÖzetBu raporda 1.5 yaşında, erkek bir dağ ceylanında saptanan generalize reaktif amiloidozis tanımlandı. Makroskobik incelemede karın boşluğundaki serozalarda hiperemi ve rumende ise kenarları kanamalı 7 cm çapında ülser belirlendi. Her iki böbreğin yüzeyinde 1-2 mm büyüklüğünde boz-beyaz renkte odaklar gözlendi. Mikroskobik incelemelerde böbreklerde glomerulus, tubulus ve akıtıcı kanal bazal membranları ve damar duvarlarında, dalakta foliküllerde, karaciğerde Disse aralığında ve ince barsaklarda lamina propriyada pembe renkte, homojen ve amorf yapıda birikimler gözlendi. Bu birikimlerin Kongo kırmızısı ile tuğla kırmızısı renkte boyandıkları, potasyum permanganat ile muamele edildikten sonra Kongo kırmızısı ile boyanmadıkları görülerek AA-amiloidoz (sekonder amiloidozis) olduğu kanısına varıldı. %10'luk formaldehitte bulunan stok doku parçalarının Lugol ve sülfirik asitle muamele edilmeleri sonucunda amiloid birikimlerinin koyu mavi renkte oldukları gözlendi. Rumende ülserasyonla birlikte generalize amiloidoz saptanan bu olguda, amiloid birikimlerinin makroskobik olarak sadece taze dokularda değil formaldehit solüsyonunda tespit edildikten sonra da gösterilebileceğine dikkat çekilmiştir. Anahtar sözcükler: Generalize amiloidozis, Ceylan, Patoloji Generalized Reactive Amyloidosis in a Gazelle (Gazella gazella) SummaryThis report was described generalized amyloidosis in a male gazelle, 1.5 years old. In necropsy, hyperaemia on all the serosal surfaces in abdomen, a hemorrhagic ulcer, 7 cm in diameter, on mucosa of rumen, and whitish colored foci, 1-2 mm diameter, on the surfaces of both kidneys were present. Microscopically, there were homogenous pink depositions in the glomeruli, intertubular interstitium, tubular basement membranes and walls of blood vessel in kidney, lymphoid follicles in spleen, space of Disse in liver and lamina propria of the small intestine. These deposits were seen brick-red color stained with Congo red on tissue sections, but these were not stained with Congo red after treated with potassium permanganate. For this reason, AA (secondary) amyloidosis was evaluated. When stock tissue samples stored in 10% formalin were treated with Lugol and acid sulphuric, amyloid depositions were seen as dark blue. Generalized reactive amyloidosis with ulceration in the rumen was diagnosed. Amyloid deposits in the tissues are not only fresh tissue but also tissue stored formalin can be determined as a macroscopic.

Pathomorphological and molecular investigations in naturally infected Chukar partridges (Alectoris Chukar) with Histomonas meleagridis and Tetratrichomonas gallinarum

“…Cases of generalized or localized amyloidosis have been reported previously in partridges (McMartin et al 1996, Kapakin et al 2010. It is known that the only type of amyloid observed in poultry is amyloid A, which is encountered in recurrent or chronic infections (McMartin et al 1996, Kapakin et al 2010, Özdemir et al 2013, Hauck et al 2019. In a study investigating the mortality of partridges with typhlohepatitis and amyloidosis, it was reported that a high rate of T. gallinarum sequence homology was obtained (Hauck et al 2019).…”