Bipotent progenitors as embryonic origin of retinal stem cells

Tang, Xia; Gao, Jianan; Jia, Xinling; Zhao, Wanli; Zhang, Yijie; Pan, Weijun; He, Jie

doi:10.1083/jcb.201611057

Cited by 29 publications

(38 citation statements)

References 87 publications

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“…Nevertheless, terminating clones arising from the very periphery of the niche unambiguously demonstrate that some stem cells failed to self-renew throughout the life of the animal. Moreover, our finding that only cells in the first two rows of the CMZ have stem cell potential is consistent with in vivo time-lapse data (Wan et al , 2016; Tang et al , 2017). Interestingly, retrograde movement of row 2 cells into row 1 of the CMZ occurs in vivo (Wan et al , 2016), which we also observed in our simulations.…”

Section: Discussionsupporting

confidence: 91%

“…In vivo , the spatial extent of the CMZ stem cell domain is believed to be defined by cues such as nearby blood vessels (Wan et al , 2016; Tang et al , 2017). Therefore, we defined the virtual stem cell domain with a fixed size of 25 μm, i.e.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, we defined the virtual stem cell domain with a fixed size of 25 μm, i.e. 5 rows of cells, reflecting the endogenous scale of the Rx2-expressing CMZ domain (Reinhardt et al , 2015; Wan et al , 2016; Tang et al , 2017). In vivo , NR stem cells divide predominantly asymmetrically, but also undergo symmetric divisions (Centanin et al , 2014).…”

Section: Resultsmentioning

confidence: 99%

“…The CMZ can be subdivided into a peripheral stem- and a central progenitor cell domain (Raymond et al , 2006; Centanin et al , 2014; Wan et al , 2016; Shi et al , 2017). At the very periphery of the CMZ, about 5 rows of cells express the stem cell marker retina-specific homeobox gene 2 (Rx2) (Reinhardt et al , 2015; Wan et al , 2016; Tang et al , 2017). The CMZ is a bi-partite niche, with tissue-specific stem cells for NR and RPE (Shi et al , 2017).…”

Section: Introductionmentioning

confidence: 99%

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Retinal stem cells modulate proliferative parameters to coordinate post-embryonic morphogenesis in the eye of fish

Tsingos

Hoeckendorf

Suetterlin

et al. 2018

Preprint

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15Different tissues coordinate growth rates by hierarchical subdivision in inducer of growth and 16 responder roles. 3D organization of the stem cell niche predisposes the retina to a spatially 17 biased neutral drift, which is differentially modulated in the neural retina and retinal pigmented 18 epithelium to synchronize growth rates, regulate organ shape, and cellular topology. 19 Abstract: 20A fundamental question in biology is how anatomically and functionally distinct tissues 21 coordinate to direct growth and shape in complex organs. We address this question using as 22

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Section: Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Retinal stem cells modulate proliferative parameters to coordinate post-embryonic morphogenesis in the eye of fish

Tsingos

Hoeckendorf

Suetterlin

et al. 2018

Preprint

View full text Add to dashboard Cite

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“…The same might be true for retinal stem cells as well. In addition, rare dormant cells have recently been described at the extreme tip of the zebrafish CMZ (Tang et al 2017), suggesting that the Xenopus retinal stem cell cohort might be composed of both "activated" proliferating cells and "resting" quiescent ones, as shown in neurogenic zones of the adult mammalian or zebrafish brain (Than-Trong and Bally-Cuif 2015; Chaker et al 2016). Finally, our personal observations suggest that the dorsal and ventral parts of the CMZ do not proliferate at the same rate, indicating an additional regional heterogeneity in cell cycle progression.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Assessment of Neural Precursor Cell Cycle Kinetics in the Amphibian Retina

Locker¹,

Perron²

2019

Cold Spring Harb Protoc

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Cell cycle progression is intimately linked to cell fate commitment during development. In addition, adult stem cells show specific proliferative behaviors compared to progenitors. Exploring cell cycle dynamics and regulation is therefore of utmost importance, but constitutes a great challenge in vivo. Here we provide a protocol for evaluating in vivo the length of all cell cycle phases of neural stem and progenitor cells in the post-embryonic Xenopus retina. These cells are localized in the ciliary marginal zone (CMZ), a peripheral region of the retina that sustains continuous neurogenesis throughout the animal's life. The CMZ bears two tremendous advantages for cell cycle kinetics analyses. First, this region, where proliferative cells are sequestered, can be easily delineated. Second, the spatial organization of the CMZ mirrors the temporal sequence of retinal development, allowing for topological distinction between retinal stem cells (residing in the most peripheral margin), and amplifying progenitors (located more centrally). We describe herein how to determine CMZ cell cycle parameters using a combination of (i) a cumulative labeling assay, (ii) the percentage of labeled mitosis calculation, and (iii) the mitotic index measurement. Taken together, these techniques allow us to estimate total cell cycle length (T C) as well as the duration of all cell cycle phases (T S/G2/M/G1). Although the method presented here was adapted to the particular system of the CMZ, it should be applicable to other tissues and developmental stages as well. MATERIALS It is essential that you consult the appropriate Material Safety Data Sheets and your institution's Environmental Health and Safety Office for proper handling of equipment and hazardous materials used in this protocol. RECIPES: Please see the end of this protocol for recipes indicated by . Additional recipes can be found online at http://cshprotocols.cshlp.org/site/recipes. Reagents 1-butanol (100%) 5-ethynyl-2 ′-deoxyuridine (EdU) (Thermo Fisher Scientific) Alexa-coupled secondary antibody (1 mg/mL) (e.g., Thermo Fischer Scientific) Antifade mounting medium (e.g., FluorSave reagent [Thermo Fischer Scientific]) Anti-phospho-Histone H3 (PH3) antibody (1 mg/mL) (e.g., Millipore 06-570)

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Multicolor strategies for investigating clonal expansion and tissue plasticity

Dumas

Clavreul

Michon

et al. 2022

Cell. Mol. Life Sci.

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Understanding the generation of complexity in living organisms requires the use of lineage tracing tools at a multicellular scale. In this review, we describe the different multicolor strategies focusing on mouse models expressing several fluorescent reporter proteins, generated by classical (MADM, Brainbow and its multiple derivatives) or acute (StarTrack, CLoNe, MAGIC Markers, iOn, viral vectors) transgenesis. After detailing the multi-reporter genetic strategies that serve as a basis for the establishment of these multicolor mouse models, we briefly mention other animal and cellular models (zebrafish, chicken, drosophila, iPSC) that also rely on these constructs. Then, we highlight practical applications of multicolor mouse models to better understand organogenesis at single progenitor scale (clonal analyses) in the brain and briefly in several other tissues (intestine, skin, vascular, hematopoietic and immune systems). In addition, we detail the critical contribution of multicolor fate mapping strategies in apprehending the fine cellular choreography underlying tissue morphogenesis in several models with a particular focus on brain cytoarchitecture in health and diseases. Finally, we present the latest technological advances in multichannel and in-depth imaging, and automated analyses that enable to better exploit the large amount of data generated from multicolored tissues.

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Bipotent progenitors as embryonic origin of retinal stem cells

Abstract: Retinal stem cells yield all retinal cell types, but their embryonic origin is elusive. Using a Zebrabow-based clonal analysis and in vivo lineage analyses, Tang et al. identify bipotent progenitors as the cell of origin of retinal stem cells.

Cited by 29 publications

References 87 publications

Retinal stem cells modulate proliferative parameters to coordinate post-embryonic morphogenesis in the eye of fish

Retinal stem cells modulate proliferative parameters to coordinate post-embryonic morphogenesis in the eye of fish

In Vivo Assessment of Neural Precursor Cell Cycle Kinetics in the Amphibian Retina

Multicolor strategies for investigating clonal expansion and tissue plasticity

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