Bipolar Patterning of Polymer Membranes by Pyroelectrification

Rega, Romina; Gennari, Oriella; Mecozzi, Laura; Grilli, Simonetta; Pagliarulo, Vito; Ferraro, Pietro

doi:10.1002/adma.201503711

Cited by 27 publications

(25 citation statements)

References 35 publications

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“…The mechanism of cell adhesion and the resulting morphology on different surfaces is complex, often dependent on a wide range of factors such as the protein species adsorbed on the surfaces [ 16 , 17 ], surface structure geometries [ 17 , 18 , 19 , 20 , 21 ], roughness [ 22 , 23 , 24 , 25 , 26 , 27 ], and surface energy of the substrata [ 22 , 28 ]. Recently, novel functional biocompatible ferroelectric materials, such as lithium niobate and lithium tantalate, have been used to manipulate cell behavior [ 29 , 30 , 31 , 32 , 33 , 34 , 35 ]. In particular, the surface charge of these materials is able to enhance osteoblast function, mineral formation [ 31 ], and create human neuroblastoma cell patterns [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, novel functional biocompatible ferroelectric materials, such as lithium niobate and lithium tantalate, have been used to manipulate cell behavior [ 29 , 30 , 31 , 32 , 33 , 34 , 35 ]. In particular, the surface charge of these materials is able to enhance osteoblast function, mineral formation [ 31 ], and create human neuroblastoma cell patterns [ 35 ]. The influences of topographic-based parallel line surface structures on cell adhesion, morphology, and behaviors have been studied by several researchers [ 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ].…”

Section: Introductionmentioning

confidence: 99%

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Nanoscale-Textured Tantalum Surfaces for Mammalian Cell Alignment

et al. 2018

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Tantalum is one of the most important biomaterials used for surgical implant devices. However, little knowledge exists about how nanoscale-textured tantalum surfaces affect cell morphology. Mammalian (Vero) cell morphology on tantalum-coated comb structures was studied using high-resolution scanning electron microscopy and fluorescence microscopy. These structures contained parallel lines and trenches with equal widths in the range of 0.18 to 100 μm. Results showed that as much as 77% of adherent cell nuclei oriented within 10° of the line axes when deposited on comb structures with widths smaller than 10 μm. However, less than 20% of cells exhibited the same alignment performance on blanket tantalum films or structures with line widths larger than 50 μm. Two types of line-width-dependent cell morphology were observed. When line widths were smaller than 0.5 μm, nanometer-scale pseudopodia bridged across trench gaps without contacting the bottom surfaces. In contrast, pseudopodia structures covered the entire trench sidewalls and the trench bottom surfaces of comb structures with line-widths larger than 0.5 μm. Furthermore, results showed that when a single cell simultaneously adhered to multiple surface structures, the portion of the cell contacting each surface reflected the type of morphology observed for cells individually contacting the surfaces.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nanoscale-Textured Tantalum Surfaces for Mammalian Cell Alignment

et al. 2018

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“…22 Exploiting its pyroelectric properties, PPLN can also be used for generating permanent charge patterns on polymer membranes, which can then be used for cell patterning. 23 Furthermore, photorefractive properties of iron-doped LiNbO 3 can be used for electrode-free immobilization of bacteria via light-induced dielectrophoresis. 24 Finally, cell sorting can be achieved via acoustic waves by bonding LiNbO 3 to microfluidic devices.…”

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confidence: 99%

Charge and topography patterned lithium niobate provides physical cues to fluidically isolated cortical axons

Kilinc

Blasiak

Baghban

et al. 2017

Applied Physics Letters

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In vitro devices that combine chemotactic and physical cues are needed for understanding how cells integrate different stimuli. We explored the suitability of lithium niobate (LiNbO3), a transparent ferroelectric material that can be patterned with electrical charge domains and micro/nanotopography, as a neural substrate. On flat LiNbO3 z-surfaces with periodically alternating charge domains, cortical axons are partially aligned with domain boundaries. On submicron-deep etched trenches, neurites are aligned with the edges of the topographical features. Finally, we bonded a bicompartmental microfluidic chip to LiNbO3 surfaces patterned by etching, to create isolated axon microenvironments with predefined topographical cues. LiNbO3 is shown to be an emerging neuron culture substrate with tunable electrical and topographical properties that can be integrated with microfluidic devices, suitable for studying axon growth and guidance mechanisms under combined topographical/chemical stimuli.

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“…The spontaneous polarization P s changes according to Δ P i α p i Δ T , where P i is the coefficient of the polarization vector, p i is the pyroelectric coefficient and Δ T is the temperature variation ( p i = −8.3 × 10 −5 C m −2 °C −1 for LN at 25 °C). At equilibrium, when the thermal stimulation is turned off, the spontaneous polarization of the LN crystal is completely screened by the external screening charge and no electric field is present [ 33 , 34 , 35 ]. When the crystal is stimulated through a temperature variation, a surface charge density α = p i Δ T appears locally due to uncompensated charges and a high electric field (E NL ~10 7 V/m) is generated on the surface of the LN crystal.…”

Section: Methodsmentioning

confidence: 99%

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

Rega

Mugnano

Oleandro

et al. 2020

Sensors

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The demand for sensors capable of measuring low-abundant collagen in human fluids has highly increased in recent years. Indeed, collagen is expected to be a biomarker for chronic diseases and could monitor their progression. Here we show detection of highly diluted samples of collagen at picogram level thanks to an innovative pyro-electrohydrodynamic jet (p-jet) system. Through the intense electric fields generated by the pyroelectric effect in a ferroelectric crystal, the collagen solution was concentrated on a small area of a slide that was appropriately functionalized to bind proteins. The collagen molecules were labeled by an appropriate fluorophore to show how the number of tiny droplets influences the limit of detection of the technique. The results show that the p-jet is extremely promising for overcoming the current detection limits of collagen-based products in human fluids, performing 10 times better than the enzyme-linked immunosorbent assay (ELISA) and thus paving the way for the early diagnosis of related chronic diseases.

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Bipolar Patterning of Polymer Membranes by Pyroelectrification

Cited by 27 publications

References 35 publications

Nanoscale-Textured Tantalum Surfaces for Mammalian Cell Alignment

Nanoscale-Textured Tantalum Surfaces for Mammalian Cell Alignment

Charge and topography patterned lithium niobate provides physical cues to fluidically isolated cortical axons

Detecting Collagen Molecules at Picogram Level through Electric Field-Induced Accumulation

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