Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II

Fang, Xiaodong; Luo, Juan; Nishihama, Ryuichi; Wloka, Carsten; Dravis, Christopher; Travaglia, Mirko; Iwase, Masayuki; Vallen, Elizabeth A.; Bi, Erfei

doi:10.1083/jcb.201005134

Cited by 105 publications

(314 citation statements)

References 61 publications

(148 reference statements)

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“…Myo1, the sole myosin-II heavy chain in budding yeast, forms a two-headed structure with a rod tail, similar to all other ''conventional'' myosin-IIs in animal cells [Fang et al, 2010]. Myo1 targets to the division site through a biphasic mechanism (Fig.…”

Section: Amr Assemblymentioning

confidence: 99%

“…Myo1 targets to the division site through a biphasic mechanism (Fig. 3) [Fang et al, 2010]. In the first phase, Myo1 targeting is mediated by the septin-binding protein Bni5 [Lee et al, 2002], which covers the time from late G1 to the onset of telophase [Fang et al, 2010].…”

Section: Amr Assemblymentioning

confidence: 99%

“…Strikingly, disruption of the AMR, for example, by deleting MYO1, which encodes the sole myosin-II gene in budding yeast, causes the formation of misoriented PS (Fig. 1D) [Rodriguez and Paterson, 1990;Fang et al, 2010], suggesting that the AMR may guide PS formation [Fang et al, 2010]. Reciprocally, blocking PS formation by deleting CHS2, which encodes the chitin synthase II in budding yeast, results in asymmetric AMR constriction, suggesting that the PS may stabilize the AMR during its constriction [Bi, 2001;Schmidt et al, 2002;VerPlank and Li, 2005].…”

Section: Introductionmentioning

confidence: 99%

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Mechanisms of cytokinesis in budding yeast

Wloka

2012

Cytoskeleton

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Cytokinesis is essential for cell proliferation in all domains of life. Because the core components and mechanisms of cytokinesis are conserved from fungi to humans, the budding yeast Saccharomyces cerevisiae has served as an attractive model for studying this fundamental process. Cytokinesis in budding yeast is driven by two interdependent cellular events: actomyosin ring (AMR) constriction and the formation of a chitinous cell wall structure called the primary septum (PS), the functional equivalent of extracellular matrix remodeling during animal cytokinesis. AMR constriction is thought to drive efficient plasma membrane ingression as well as to guide PS formation, whereas PS formation is thought to stabilize the AMR during its constriction. Following the completion of the PS formation, two secondary septa (SS), consisting of glucans and mannoproteins, are synthesized at both sides of the PS. Degradation of the PS and a part of the SS by a chitinase and glucanases then enables cell separation. In this review, we discuss the mechanics of cytokinesis in budding yeast, highlighting its common and unique features as well as the emerging questions. © 2012 Wiley Periodicals, Inc.

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Section: Amr Assemblymentioning

confidence: 99%

Section: Amr Assemblymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mechanisms of cytokinesis in budding yeast

Wloka

2012

Cytoskeleton

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“…However, two sequential and independent pathways promote myosin accumulation to this site: One pathway is active during G 1 , and the second is active during anaphase; only the latter is required for cytokinesis (Fang et al 2010). Other important events in ring assembly are temporally regulated by a polo kinase ortholog, Cdc5.…”

Section: Temporal Control Of Cytokinesis In Budding Yeastmentioning

confidence: 99%

“…Remarkably, the requirement for Myo1 can be provided by a headless version lacking the motor domain. This truncated myosin is therefore not performing a filament-cross-linking function-more likely it functions as a scaffold that guides deposition of septal materials (Lord et al 2005;Fang et al 2010;Wloka et al 2013). Although headless myosin fulfills its essential function, loss of the head has consequences-for example, the motor domain of myosin is needed to accelerate ring constriction and the rate of actin disassembly during constriction (Mendes Pinto et al 2012).…”

Section: Cytokinesis In Metazoa and Fungimentioning

confidence: 99%

Cytokinesis in Metazoa and Fungi

Glotzer

2016

Cold Spring Harb Perspect Biol

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SUMMARYCell division-cytokinesis-involves large-scale rearrangements of the entire cell. Primarily driven by cytoskeletal proteins, cytokinesis also depends on topological rearrangements of the plasma membrane, which are coordinated with nuclear division in both space and time. Despite the fundamental nature of the process, different types of eukaryotic cells show variations in both the structural mechanisms of cytokinesis and the regulatory controls. In animal cells and fungi, a contractile actomyosin-based structure plays a central, albeit flexible, role. Here, the underlying molecular mechanisms are summarized and integrated and common themes are highlighted.

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Comparing contractile apparatus‐driven cytokinesis mechanisms across kingdoms

et al. 2012

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Cytokinesis is the final stage of the cell cycle during which a cell physically divides into two daughters through the assembly of new membranes (and cell wall in some cases) between the forming daughters. New membrane assembly can either proceed centripetally behind a contractile apparatus, as in the case of prokaryotes, archaea, fungi, and animals or expand centrifugally, as in the case of higher plants. In this article, we compare the mechanisms of cytokinesis in diverse organisms dividing through the use of a contractile apparatus. While an actomyosin ring participates in cytokinesis in almost all centripetally dividing eukaryotes, the majority of bacteria and archaea (except Crenarchaea) divide using a ring composed of the tubulin-related protein FtsZ. Curiously, despite molecular conservation of the division machinery components, division site placement and its cell cycle regulation occur by a variety of unrelated mechanisms even among organisms from the same kingdom. While molecular motors and cytoskeletal polymer dynamics contribute to force generation during eukaryotic cytokinesis, cytoskeletal polymer dynamics alone appears to be sufficient for force generation during prokaryotic cytokinesis. Intriguingly, there are life forms on this planet that appear to lack molecules currently known to participate in cytokinesis and how these cells perform cytokinesis remains a mystery waiting to be unravelled. V C 2012 Wiley Periodicals, Inc Key Words: cytokinesis, cytoskeleton, contractile apparatus Introduction C ytokinesis is a fundamental cellular process essential for cell proliferation in all life forms. In addition, proper regulation of the site of cytokinesis is important for cell fate establishment in several developmental contexts across kingdoms. All cells, with the exception of plants, use a contractile apparatus placed at a chosen division site to accomplish cytokinesis. Contraction and closure of this cell division apparatus facilitates assembly of new membranes and the completion of cytokinesis. A number of informative review articles in this issue of Cytoskeleton describe in detail mechanisms of cytokinesis in various organisms. In our review article, we compare contractile apparatus-driven cytokinesis regulatory mechanisms across kingdoms, by considering the process in bacteria, archaea, fungi, and animals. We discuss four major aspects of cytokinesis: (1) Composition of the cytokinetic apparatus, (2) Mechanism of positioning of the cytokinetic apparatus, (3) Mechanism of assembly of the cytokinetic apparatus, and (4) Mechanism of force generation by the cytokinetic apparatus. Modes of generation of constrictive forces, other than those by the cytoskeletonbased cytokinetic apparatus, are being discovered in recent times. While these are mentioned briefly in this article, we restrict this review to describe assembly and force generation by cytoskeleton-based cell division apparatuses. Composition of the Cytokinetic ApparatusThe cytoskeleton that functions in cytokinesis in nearly all cell types fal...

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Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II

Abstract: The tail of yeast myosin II is localized to the division site by two distinct molecular pathways and sufficient for promoting actomyosin ring assembly, furrow ingression, and guidance in ECM remodeling.

Cited by 105 publications

References 61 publications

Mechanisms of cytokinesis in budding yeast

Mechanisms of cytokinesis in budding yeast

Cytokinesis in Metazoa and Fungi

Comparing contractile apparatus‐driven cytokinesis mechanisms across kingdoms

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