Cytokinesis in Metazoa and Fungi

Glotzer, Michael

doi:10.1101/cshperspect.a022343

Cited by 69 publications

(87 citation statements)

References 178 publications

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“…Fundamental aspects of cytokinesis are conserved between yeast and animal cells (Balasubramanian et al, 2004;Bhavsar-Jog and Bi, 2017;Glotzer, 2016). Both yeast and metazoan cells undergo cytokinesis through a sequence of coordinated steps that include AMR contraction, membrane ingression, localized secretion, extracellular matrix (ECM) remodeling, and abscission.…”

Section: How Broadly Conserved Is the Eco Pathway?mentioning

confidence: 99%

“…Both yeast and metazoan cells undergo cytokinesis through a sequence of coordinated steps that include AMR contraction, membrane ingression, localized secretion, extracellular matrix (ECM) remodeling, and abscission. Mechanisms to ensure coordinated execution of these steps are beginning to be understood (Glotzer, 2016;Neto and Gould, 2011). For example, kinases play a fundamental role in ensuring abscission order (Paolo D'Avino and Capalbo, 2016).…”

Section: How Broadly Conserved Is the Eco Pathway?mentioning

confidence: 99%

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A cell separation checkpoint that enforces the proper order of late cytokinetic events

Brace¹,

Doerfler²,

Weiss³

2018

Preprint

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List of Abbreviations:Actomyosin ring (AMR); Primary septum (PS); Secondary septum (SS); Cyclin dependent kinase (CDK); Auxin inducible degron (AID); Immunoprecipitation (IP); Mitotic Exit Network (MEN); Regulation of Ace2 and Morphogenesis network (RAM) SummaryEukaryotic cell division requires sequence dependency relationships in which late processes commence only after early ones are appropriately completed. We have discovered a system that blocks late events of cytokinesis until early ones are successfully accomplished. In budding yeast, cytokinetic actomyosin ring contraction and membrane ingression are coupled with deposition of an extracellular septum that is destroyed immediately after its completion by secreted enzymes. We find this secretion event is linked to septum completion and forestalled when the process is slowed. Delay of septum destruction requires Fir1, an intrinsically disordered protein localized to the cytokinesis site that is degraded upon septum completion but stabilized when septation is aberrant. Fir1 appears to protect cytokinesis in part by inhibiting a separation-specific exocytosis function of the NDR/LATS kinase Cbk1, a key component of a "hippo" signaling pathway that induces mother-daughter separation. We term this system "enforcement of cytokinesis order" (ECO), a checkpoint ensuring proper temporal sequence of mechanistically incompatible processes of cytokinesis.Eukaryotic cells undergo dramatic reorganization at the end of mitosis, producing two cells from one through the processes of cytokinesis. This division requires execution of mechanistically diverse events in an unvarying and often rapid sequence, in which late processes are not initiated until early ones are effectively completed. Specification of the division site and assembly of cytokinetic structures precedes the mechanical and regulatory events of actomyosin ring (AMR) constriction and membrane ingression; this is followed by disassembly of cytokinetic machinery, cessation of cytokinetic membrane trafficking, and abscission of the divided cells (

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Section: How Broadly Conserved Is the Eco Pathway?mentioning

confidence: 99%

Section: How Broadly Conserved Is the Eco Pathway?mentioning

confidence: 99%

A cell separation checkpoint that enforces the proper order of late cytokinetic events

Brace¹,

Doerfler²,

Weiss³

2018

Preprint

View full text Add to dashboard Cite

show abstract

“…The transition also involves changes in the actin and microtubule cytoskeleton (Hu et al, 2012;Terry et al, 2018). A complex machinery is involved in organizing the succession of events during cytokinesis, ensuring that they proceed with high fidelity (Fededa and Gerlich, 2012;Green et al, 2012;D'Avino et al, 2015;Glotzer, 2017). Activation of the small molecular weight GTPase RhoA is an early event essential for furrow initiation and CR formation (Bement et al, 2005;Piekny et al, 2005;Basant and Glotzer, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Activation of the small molecular weight GTPase RhoA is an early event essential for furrow initiation and CR formation (Bement et al, 2005;Piekny et al, 2005;Basant and Glotzer, 2018). The CR comprises filament-forming cytoskeletal proteins including actin, myosin II and septins, along with many other accessory proteins that ensure CR contractility, attachment to the plasma membrane, and interaction with central spindle microtubules (Green et al, 2012;D'Avino et al, 2015;Glotzer, 2017). Among these CR proteins is the Citron kinase, named Sticky in Drosophila melanogaster.…”

Section: Introductionmentioning

confidence: 99%

Rho-dependent control of the Citron kinase, Sticky, drives midbody ring maturation

El-Amine

Carim

Wernike

et al. 2019

Preprint

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Rho-dependent proteins control assembly of the cytokinetic contractile ring (CR), yet it remains unclear how those proteins guide ring closure and how they promote subsequent formation of a stable midbody ring (MR). Citron kinase is one important component required for MR formation but its mechanisms of action and relationship with Rho are controversial.Here, we conduct a structure-function analysis of the Drosophila Citron kinase, Sticky, in Schneider's S2 cells. We define two separable and redundant RhoGEF/Pebble-dependent inputs into Sticky recruitment to the nascent MR and show that each input is subsequently required for retention at, and for the integrity of, the mature MR. The first input is via an actomyosin-independent interaction between Sticky and Anillin, a key scaffold also required for MR formation. The second input requires the Rho-binding domain of Sticky, whose boundaries we have defined. Collectively, these results show how MR biogenesis depends on the coordinated actions of Sticky, Anillin and Rho.

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“…Similarly, ER localized Kif2A, a microtubule depolymerase, induces spindle asymmetry and positioning in ascidians (Costache et al, 2017). Since the anaphase spindle is the primary determinant for the positioning of the cleavage furrow (D'Avino et al, 2015;Glotzer, 2017;Green et al, 2012;Rappaport, 1986), spindle geometry can at least partially explain physical asymmetry.…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporally controlled Myosin relocalization and internal pressure cause biased cortical extension to generate sibling cell size asymmetry

Pham

Monnard

Helenius

et al. 2018

Preprint

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Metazoan cells can generate unequal sized sibling cells during cell division. This form of asymmetric cell division depends on spindle geometry andMyosin distribution but the underlying mechanics are unclear. Here, we use atomic force microscopy and live cell imaging to elucidate the biophysical forces involved in the establishment of physical asymmetry in Drosophila neural stem cells. We show that the force driving initial apical membrane expansion is provided by hydrostatic pressure, peaking shortly after anaphase onset, and enabled by a relieve of actomyosin contractile tension on the apical cell cortex. The subsequent increase in contractile forces at the cleavage furrow, combined with the relocalization of basally located Myosin results in basal membrane extension and sustained apical expansion. We propose that spatiotemporally controlled actomyosin contractile tension and hydrostatic pressure enables stereotypic biased membrane expansion to generate sibling cell size asymmetry.

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Cytokinesis in Metazoa and Fungi

Cited by 69 publications

References 178 publications

A cell separation checkpoint that enforces the proper order of late cytokinetic events

A cell separation checkpoint that enforces the proper order of late cytokinetic events

Rho-dependent control of the Citron kinase, Sticky, drives midbody ring maturation

Spatiotemporally controlled Myosin relocalization and internal pressure cause biased cortical extension to generate sibling cell size asymmetry

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