Biphasic Regulation of Leukocyte Superoxide Generation by Nitric Oxide and Peroxynitrite

Lee, Chang Il; Miura, Katsushi; Li, Xiaoping; Zweíer, Jay L.

doi:10.1074/jbc.m006341200

Cited by 118 publications

(96 citation statements)

References 59 publications

(75 reference statements)

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“…Neutrophils have also been coupled commonly with inflammatory conditions, which are mostly associated with the accumulation of NO and its metabolites. Various reports demonstrated NOmediated biphasic regulation (14,16,17), stimulation (15,22), and even inhibition (18,20) of PMNs reactive species formation. These outcomes could be due to the differences in probes used to detect ROS/RNS, which exhibit different reactivity, specificity, and selectivity, and some probes even interact with NO (25,26).…”

Section: Discussionmentioning

confidence: 99%

“…Studies from this laboratory as well as from others have demonstrated a biphasic role of NO on the reactive species formation in PMNs in response to arachidonic acid, fMLP, and PMA stimulation (14)(15)(16)(17). Low concentrations (10-100 nM) of NO potentiated reactive species generation by augmented ADP-ribosylation, intracellular calcium, and activation of ERK MAPK pathway, but higher concentrations (1 lM-1 mM) attenuated ROS generation through feed back inhibition of NADPH-oxidase and superoxide scavenging effect and activation of the guanylate cyclase-cyclic GMP pathway from the rat and human PMNs (15,16,(18)(19)(20). Indeed, NO suppressed NADPH oxidase-dependent superoxide production by S-nitrosylation of p47 subunit in human endothelial cells (21).…”

mentioning

confidence: 96%

“…), absorbance (NBT, cytochrome c reduction), or electron paramagnetic resonance (EPR; by utilizing spin traps, DMPO/ DEPMPO) (15,16,(18)(19)(20)23,24). The ROS/RNS species have different sensitivity/specificity for these probes/methods like O 2 2 (DHE, lucigenin, NBT, cytochrome c reduction, and DEPMPO), H 2 O 2 (DCF, DHR, luminol, and Scopoletin), ONOO 2 (DCF, DHR, and luminol), OH (DCF, luminol, and DMPO), HOCl (DCF, DHR, and luminol), etc.…”

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confidence: 99%

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Nitric oxide‐mediated augmentation of neutrophil reactive oxygen and nitrogen species formation: Critical use of probes

et al. 2010

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Previous reports from this laboratory and others demonstrated NO-mediated biphasic modulation of NADPH oxidase and attenuation of neutrophil reactive oxygen species generation, whereas recently we reported augmentation in DCF fluorescence following NO treatment. These discrepancies seem to be due to utilization of different probes/ methods to assess effect of NO on reactive oxygen and nitrogen species (ROS/RNS, reactive species) generation. This study aims to look into this and evaluate NO-mediated enzymatic reactive species formation by using multiple probes, human neutrophils/ HL60 cells and various interventions. Addition of NO donor, SNP or SNAP (100 nM-1 mM) to PMNs suspension, exhibited a concentration-and time-dependent augmentation in DCF fluorescence, but reduced DHE fluorescence. Collective generation of reactive species was confirmed by enhanced DMPO-nitrone adduct, dityrosine and rhodamine-123 and quenching of scopoletin. NO also enhanced bacterial killing, without altering phagocytosis. Addition of NO to HL-60 cells lacking functional NADPH oxidase enhanced reactive species formation, indicating importance of other enzyme(s) too. NO-dependent ROS/RNS generation was substantially reduced by NADPH oxidase inhibitor (DPI), MPO inhibitor (ABAH), or NOS inhibitor (7-NI). However, 7-NI reduced MPO activity, warranting reappraisal of those reports, which implied NOS in reactive species formation. The results obtained demonstrated NO-mediated reactive species augmentation in human PMNs. Furthermore, superoxide scavenging by NO seems to be the key process in the decrease of DHE fluorescence and suggest usefulness of DCF as the most appropriate probe to measure the NO-mediated modulation of reactive oxygen species in particular in various pathological situations. ' 2010 International Society for Advancement of Cytometry

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Section: Discussionmentioning

confidence: 99%

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confidence: 96%

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Nitric oxide‐mediated augmentation of neutrophil reactive oxygen and nitrogen species formation: Critical use of probes

et al. 2010

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“…In investigations with murine leukocyte cultures suppression of oxygen radical production at intensifying of NO synthesis is revealed 26 . It is known that the physiological concentrations of NO produced by the activated granulocytes lead to increase of peroxynitrite formation, and this potentiates O 2 -formation while high NO levels suppress it 27 . In contrast to the control group, in patients with RF in remission mentioned interrelations were absent.…”

Section: Discussionmentioning

confidence: 99%

Nitric Oxide Production Disorders in Leukocytes of Patients With Recurrent Furunculosis

Hamaliaka¹,

Новикова²

2010

BIOMED PAP

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Aim. The prop ensity for certain individuals to develop staphylococcal recurrent furunculosis (RF) is not fully understood. But among the reasons of its development the immune system dysfunctions are described. As in the staphylococcus elimination the main role is played by neutrophils, the objective of this study was to determine nitric oxide (NO) and reactive oxygen species (ROS) production by polymo rphonuclear leukocytes (PMNs) of patients with RF and to compare them with the response of normal cells to stimulation.Materials and methods. The spontaneous and pyrogenal-stimulated nitric oxide production was evaluated in leukocyte cell cultures by Griess reaction, and ROS generation was determined in the stimulated and unstimulated NBT-test.Results. In this study we have demonstrated that leukocytes of healthy subjects respond on stimulation by the increase of both NO and ROS production. In contrast, leukocytes of patients with RF react by depression of NO formation at stimulation, and are characterized by decrease of ROS production reserve with the increasing of spontaneous ROS generation. Described disorders are revealed in the remission period as well as at exacerbation of furunculosis.Conclusion. The present study suggests that leukocytes of patients with RF have stable defect of stimulated NO production increase, which can be the reason for recurrent and severe course of furunculosis.

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“…This effect was concentration-dependent up to 100 μM SNAP followed by increasing apoptosis with concentrations of SNAP about 300 to 1,000 μM. This biphasic effect of NO on T cell function is reminiscent of the biphasic actions of NO seen in other blood cells which indicated stimulation at low concentrations and inhibition at high concentrations of NO [141][142][143][144][145]. Furthermore, the finding that proliferation was inhibited when SNAP was added 24 hours after stimulation suggests that NO acts at a later stage to limit proliferation rather than at the initial induction stage [133].…”

Section: No Actions On Inflammatory Cells In Acute Cardiac Rejectionmentioning

confidence: 99%

The complex role of iNOS in acutely rejecting cardiac transplants

Pieper

Roza

2008

Free Radical Biology and Medicine

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This review summarizes the evidence for a detrimental role of nitric oxide (NO) derived from inducible NO synthase (iNOS) and/or reactive nitrogen species such as peroxynitrite in acutelyrejecting cardiac transplants. In chronic cardiac transplant rejection, iNOS may have an opposing beneficial component. The purpose of this review is primarily to address issues related to acute rejection which is a recognized risk factor for chronic rejection. The evidence for a detrimental role is based upon strategies involving non-selective NOS inhibitors, NO neutralizers, selective iNOS inhibitors and iNOS gene deletion in rodent models of cardiac rejection. The review is discussed in the context of the impact on various components including graft survival, histological rejection and cardiac function which may contribute in toto to the process of graft rejection. Possible limitations of each strategy are discussed in order to understand better the variance in published findings including issues related to the potential importance of cell localization of iNOS expression. Finally, the concept of a dual role of NO and its down-stream product, peroxynitrite, in rejection vs. immune regulation is discussed.

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Biphasic Regulation of Leukocyte Superoxide Generation by Nitric Oxide and Peroxynitrite

Cited by 118 publications

References 59 publications

Nitric oxide‐mediated augmentation of neutrophil reactive oxygen and nitrogen species formation: Critical use of probes

Nitric oxide‐mediated augmentation of neutrophil reactive oxygen and nitrogen species formation: Critical use of probes

Nitric Oxide Production Disorders in Leukocytes of Patients With Recurrent Furunculosis

The complex role of iNOS in acutely rejecting cardiac transplants

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