Biphasic positive effect of formononetin on metabolic activity of human normal and osteoarthritic subchondral osteoblasts

Huh, Jungwon; Dong-Min, Seo; Baek, Yong-Hyeon; Choi, Dong‐Hee; Park, Dong-Suk; Lee, Jae-Dong

doi:10.1016/j.intimp.2010.01.012

Cited by 37 publications

(33 citation statements)

References 29 publications

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“…For instance, calycosin possesses distinct estrogenic properties (Chen et al 2011), whereas its 7- O - β -glucoside was shown to alleviate pathologic changes in rabbit model of osteoarthritis (Choi et al 2007). Another methoxylated isoflavone, formononetin, proved to inhibit bone loss in ovariectomised rats (Ha et al 2010) and exert positive effects on the metabolism of human osteoarthritic osteoblasts (Huh et al 2010). Pseudobaptigenin exhibits antiproliferative effects on selected human stomach and colon cancer cell lines (Yanagihara et al 1993) and recent bioinformatics study showed that it may act as protein tyrosine kinase inhibitor (Hashemi et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Isoflavone production in Cyclopia subternata Vogel (honeybush) suspension cultures grown in shake flasks and stirred-tank bioreactor

Kokotkiewicz

Łuczkiewicz

Kowalski

et al. 2013

Appl Microbiol Biotechnol

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Suspension cultures of the endemic South-African plant Cyclopia subternata were established for the first time and evaluated for the presence of isoflavones. The influence of light, as well as medium supplementation strategies with phenylalanine, casein hydrolysate and coconut water on biomass growth and isoflavone production were examined. The highest levels of 7-O-β-glucosides of calycosin, pseudobaptigenin and formononetin (275.57, 125.37 and 147.28 mg/100 g DW, respectively) were recorded for cultures grown in the absence of light, whereas coconut water substantially promoted biomass growth. Cell suspensions were subsequently grown in the 2-l stirred-tank bioreactor. Maximum productivity of 7-O-β-glucosides of calycosin, pseudobaptigenin and formononetin (0.96, 0.44 and 0.22 mg l−1 day−1, respectively) in bioreactor-cultivated cells was obtained for biomass grown in the dark and supplemented with coconut water. The results indicate that C. subternata suspension cultures can be utilised for the production of the specified isoflavone derivatives absent in the intact plant.

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Section: Introductionmentioning

confidence: 99%

Isoflavone production in Cyclopia subternata Vogel (honeybush) suspension cultures grown in shake flasks and stirred-tank bioreactor

Kokotkiewicz

Łuczkiewicz

Kowalski

et al. 2013

Appl Microbiol Biotechnol

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“…The first scientific reports about beneficial effect of formononetin on bone tissue focused on in vitro studies of bone cells [15, 22, 42] and on bone histomorphometric parameters of laboratory animals [13, 38]. Ha et al [13] showed that intraperitoneally administered formononetin at doses of 1 and 10 mg/kg leads to an increase of the trabeculae area in tibia and lumbar vertebra.…”

Section: Discussionmentioning

confidence: 99%

“…Formononetin has been proven to show antimicrobial activity towards Giardia lamblia [16], acting as both an antioxidant [20] and as a neuroprotective agent in Alzheimer's disease [21]. This isoflavonoid has also been reported to have positive effect on osteoarthritis [15] and promote early fracture healing through stimulating angiogenesis [22]. What is more, it can be used in cardiovascular diseases as vasorelaxant [14] as well as in the prevention of myocardial infraction due to its cardioprotective activity [23].…”

Section: Introductionmentioning

confidence: 99%

Effect of Formononetin on Mechanical Properties and Chemical Composition of Bones in Rats with Ovariectomy-Induced Osteoporosis

Kaczmarczyk-Sedlak

Wojnar

Zych

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Formononetin is a naturally occurring isoflavone, which can be found in low concentrations in many dietary products, but the greatest sources of this substance are Astragalus membranaceus, Trifolium pratense, Glycyrrhiza glabra, and Pueraria lobata, which all belong to Fabaceae family. Due to its structural similarity to 17β-estradiol, it can mimic estradiol's effect and therefore is considered as a “phytoestrogen.” The aim of this study was to examine the effect of formononetin on mechanical properties and chemical composition of bones in rats with ovariectomy-induced osteoporosis. 12-week-old female rats were divided into 4 groups: sham-operated, ovariectomized, ovariectomized treated with estradiol (0.2 mg/kg) and ovariectomized treated with formononetin (10 mg/kg). Analyzed substances were administered orally for 4 weeks. Ovariectomy caused osteoporotic changes, which can be observed in bone biomechanical features (decrease of maximum load and fracture load and increase of displacements for maximum and fracture loads) and bone chemical composition (increase of water and organic fraction content, while a decrease of minerals takes place). Supplementation with formononetin resulted in slightly enhanced bone mechanical properties and bone chemistry improvement (significantly lower water content and insignificantly higher mineral fraction content). To summarize, administration of formononetin to ovariectomized rats shows beneficial effect on bone biomechanical features and chemistry; thus, it can prevent osteoporosis development.

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“…When tested on human chondrocytes and chondrocytic cell line CHON-002, bavachin, a flavonid phytoestrogen isolated from Psoralea corylifolia, potentially protected cartilage from inflammation-mediated damage in joints of OA through decreasing IL-1beta-induced activation of IKK-IB alpha-NF-B signaling pathway (Cheng et al, 2010). Formononetin, a phytoestrogen isolated from Astragalus membranaceus s h o w e d t o h a v e biphasic positive effects on human normal osteoblasts and OA sub-chondral osteoblasts by modifying their biological synthetic capacities (Huh et al, 2010). Using female bovine articular chondrocytes, it has been demonstrated that the stimulating effect of insulin on GAGs sulfate incorporation was enhanced significantly after preincubation of cells with 10 -11 -10 -5 M daidzein or 10 -9 -10 -5 M genistein but not by 17-E2 (Claassen et al, 2008).…”

Section: Phytoestrogen and Selective Estrogen Receptor Modulators (Serm)mentioning

confidence: 99%

Estrogens Involvement in the Physiopathology of Articular Cartilage

Moslemi¹,

Demoor²,

Boumediene³

et al. 2011

Challenges in Rheumatology

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Biphasic positive effect of formononetin on metabolic activity of human normal and osteoarthritic subchondral osteoblasts

Cited by 37 publications

References 29 publications

Isoflavone production in Cyclopia subternata Vogel (honeybush) suspension cultures grown in shake flasks and stirred-tank bioreactor

Isoflavone production in Cyclopia subternata Vogel (honeybush) suspension cultures grown in shake flasks and stirred-tank bioreactor

Effect of Formononetin on Mechanical Properties and Chemical Composition of Bones in Rats with Ovariectomy-Induced Osteoporosis

Estrogens Involvement in the Physiopathology of Articular Cartilage

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