Biphasic Mechanical Response of the Isolated Vas Deferens to Nerve Stimulation

Swedin, Göran

doi:10.1111/j.1748-1716.1971.tb04936.x

Cited by 48 publications

(27 citation statements)

References 4 publications

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“…The a-receptor blocking agent thymoxamine, which antagonizes noradrenaline, had a greater antagonistic effect on the B than on the A component. Similar findings were reported by Swedin (1971) who found phentolamine to depress the B component and to have no effect on, or even to potentiate, the A component. We confirmed this finding with phentolamine on the vasa from two guinea-pigs and two rats; at a concentration of 5 x 10im phentolamine the A component was generally less inhibited than the B component.…”

Section: A T Birmingham and M Anne Freeman Resultssupporting

confidence: 76%

“…In the same way Farnebo & Malmfors (1971), who demonstrated a biphasic response in the mouse vas deferens to transmural stimulation, found that phentolamine or phenoxybenzamine blocked only the second phase of the response. Such pharmacological analysis of the A component as has been made (Swedin, 1971;Farnebo & Malmfors, 1971) suggests that it has a different mechanism from the B component, being, for example, abolished by prostaglandin E, which leaves the B component unaffected. Ambache & Zar (1971) showed that the tension developed by the transmurally stimulated guinea-pig vas is influenced by the number of pulses in a train, the frequency of stimulation, and the duration of each pulse and suggested on the basis of their findings that two different groups of nerve fibres may be involved, each of differing size and therefore of different excitability.…”

Section: A T Birmingham and M Anne Freeman Resultsmentioning

confidence: 99%

“…No formal analysis of the phenomenon appeared until Swedin (1971) studied the effect of train length on electrical stimulation of guinea-pig and rat vasa. He found at 370 C that two components to the response were present at all frequencies of stimulation from 4 to 25 Hz for a train of pulses lasting 30 sec, whereas for a 5 see train of pulses the response consisted of the first, rapid component only.…”

Section: A T Birmingham and M Anne Freeman Resultsmentioning

confidence: 99%

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The relation between stimulus frequency and the relative size of the components of the biphasic response of the vas deferens to electrical stimulation at different temperatures.

Birmingham¹,

Freeman²

1976

The Journal of Physiology

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SUMMARY1. Electrical stimulation of the guinea-pig or rat vas deferens (pre-or post-ganglionically) at frequencies from 2-5 to 40 Hz with trains of stimuli of 30 see duration induced a biphasic response. A rapid contraction (component A) was followed after a brief relaxation by a slower contraction (component B); the two phases were seen most clearly with stimulation frequencies of less than 10 Hz.2. The responses to post-ganglionic stimulation were always larger than those to preganglionic stimulation. In general, at low frequencies component A exceeded component B whilst at high frequencies component B was the larger. Separation of the two components on the basis of their frequency response characteristics was better for rat than for guinea-pig vasa.3. Log. frequency-response curves to transmural (post-ganglionic) electrical stimulation and log dose-response curves to noradrenaline were recorded for guinea-pig and rat vasa deferentia at 320, 220 and 120 C.For the guinea-pig reduction of bath temperature to 120 C increased the amplitude of component A at 2-5 and 5 Hz; component B could not confidently be distinguished at this temperature. At 220 C there was potentiation of B at lower frequencies and depression of B at higher frequencies. There was no response to noradrenaline at 120 C. At 220 C the response to noradrenaline was increased except to doses at or near the maximum to which the response was reduced.4. For the rat vas deferens component A was little changed by reduction of temperature. Component B at 120 C was greatly depressed at higher frequencies. The response to noradrenaline was increased to lower doses and decreased to higher doses as the temperature was lowered. 748A. T. BIRMINGHAM AND M. ANNE FREEMAN 5. The B component of the response of guinea-pig vasa at 220 C and rat vasa at 320 C was more sensitive than the A component to inhibition by thymoxamine.6. Further analysis of the mechanisms underlying the A and B components of the biphasic response may be facilitated by relative isolation of each component by the appropriate selection of parameters of electrical stimulation and of temperature for the species being investigated. The contractions of the B component are similar to, if not identical with, those produced by exogenously applied noradrenaline.

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Section: A T Birmingham and M Anne Freeman Resultssupporting

confidence: 76%

Section: A T Birmingham and M Anne Freeman Resultsmentioning

confidence: 99%

Section: A T Birmingham and M Anne Freeman Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The relation between stimulus frequency and the relative size of the components of the biphasic response of the vas deferens to electrical stimulation at different temperatures.

Birmingham¹,

Freeman²

1976

The Journal of Physiology

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“…However, there was a concomitant increase in sensitivity to exogenous noradrenaline of about 20 fold and if a similar increase in sensitivity to endogenous noradrenaline is assumed, then an estimate of 99% inhibition of normal nerve function may be nearer the truth. Fluorescence-histochemistry gives little help in deciding which of these figures is more nearly correct since this technique, whilst giving an indication of the noradrenaline content of the tissue, cannot show the functional availability of the transmitter and it is well known that tissue noradrenaline content is not directly related to nerve function (Swedin, 1971 ;Wakade & Krusz, 1972). However, it is evident that although a high degree of sympathectomy may have been achieved it was by no means complete unless some nonadrenergic neurones accounted for the residual responses.…”

Section: Discussionmentioning

confidence: 99%

An assessment of sympathetic function in isolated tissues from mice given nerve‐growth‐factor antiserum and 6‐hydroxydopamine

Hughes

Kirk

Kneen

et al. 1973

British J Pharmacology

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Summary1. Experiments were done on isolated tissues from mice injected with 09% w/v NaCl solution (saline), 6-hydroxydopamine (6-OHDA), nerve-growthfactor antiserum (NGF-As) or a combination of these agents (NGF-As+ 6-OHDA).2. Fluorescence histochemistry of vasa deferentia showed clear differences between each of the treatments but no such distinction was possible in cardiac ventricle or intestine. 3. Compared with controls, the chronotropic responses of atria to field stimulation were reduced by all three treatments in the order NGF-As< 6-OHDA

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“…The mechanical response of the rat vas deferens to hypogastric nerve or field stimulation is biphasic (Swedin, 1971;Anton, Duncan & McGrath, 1977;. The two components underlying the two phases of the response can best be revealed when responses are elicited by single electrical stimuli rather than by trains of pulses .…”

Section: Introductionmentioning

confidence: 99%

Effects of prostaglandin E₂ on fast and slow components of the response of the rat vas deferens to field stimulation

Bedwani¹,

Blanning²

1983

British J Pharmacology

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1The response of the rat vas deferens to field stimulation with single pulses is biphasic. The first, rapid component of the response is dominant at the prostatic end and is thought by some to be mediated by non-adrenergic, non-cholinergic neurotransmission. The second, slower component predominates at the epididymal end and has the usual properties associated with adrenergic responses.2 Prostaglandin E2 (PGE2, 10-8 to 0-5 mol/l) inhibited the 'fast component' responses elicited by field stimulation of the prostatic vas from reserpine-treated rats. The prostaglandin was more potent in the presence of 1.25 than 2.5 mmol/l Ca2+.3 PGE2 (10-7 to 10`mol/l) had similar effects on prostatic preparations from normal rats, but was less active on these.4 PGE2 (10-9 to 10-5 mol/l) did not inhibit the 'slow component' responses resulting from field stimulation of the epididymal vas but rather, at the two highest concentrations used (5 x 10-6 and i0-I mol/l), potentiated them. These high concentrations of the prostaglandin also potentiated the contractions of the epididymal vas elicited by exogenous noradrenaline. 5 These effects of PGE2 on the two components of the response of the rat vas deferens are not as might be expected from its inhibitory effects on the 'typical' adrenergic neurotransmission in several other sympathetically-innervated tissues.

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Biphasic Mechanical Response of the Isolated Vas Deferens to Nerve Stimulation

Cited by 48 publications

References 4 publications

The relation between stimulus frequency and the relative size of the components of the biphasic response of the vas deferens to electrical stimulation at different temperatures.

The relation between stimulus frequency and the relative size of the components of the biphasic response of the vas deferens to electrical stimulation at different temperatures.

An assessment of sympathetic function in isolated tissues from mice given nerve‐growth‐factor antiserum and 6‐hydroxydopamine

Effects of prostaglandin E₂ on fast and slow components of the response of the rat vas deferens to field stimulation

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Biphasic Mechanical Response of the Isolated Vas Deferens to Nerve Stimulation

Cited by 48 publications

References 4 publications

The relation between stimulus frequency and the relative size of the components of the biphasic response of the vas deferens to electrical stimulation at different temperatures.

The relation between stimulus frequency and the relative size of the components of the biphasic response of the vas deferens to electrical stimulation at different temperatures.

An assessment of sympathetic function in isolated tissues from mice given nerve‐growth‐factor antiserum and 6‐hydroxydopamine

Effects of prostaglandin E2 on fast and slow components of the response of the rat vas deferens to field stimulation

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Effects of prostaglandin E₂ on fast and slow components of the response of the rat vas deferens to field stimulation