Biotransformation Using Plant Cell Culture Systems and Tissues

Abstract

Biotransformations are chemical reactions catalyzed by cells, organs or enzymes and represent an area of biotechnology that has received considerable attention. The use of biotransformations with plant cell culture systems and fragments of plant tissue has immense potential for the production of compounds with commercial interest, especially considering the vast biochemical capability for the production of secondary metabolites from plant sources. In this context, this chapter evaluates the application of biotransformations in different plant cell culture systems, such as cell suspensions, hairy roots and cell immobilization, as well as fragments of plant tissue.

The Eff ect of Precursors and Elicitors on the Production of Oleanolic Acid in Suspension Culture of Lantana camara

“…Suspension culture is convenient for a continuous supply of secondary metabolites in large quantities. 7,8 Precursor feeding induces high levels of secondary product formation in intrinsically low-producing cultures. Terpenoids, the major constituents of secondary metabolites, are biosynthesized from acetyl-CoA and are generally insoluble in water.…”

Effects of different factors on friable callus induction and establishment of cell suspension culture of Hovenia dulcis (Rhamnaceae)

“…In vitro systems may improve the accumulation of compounds when compared to the wild plant (Cai et al 2012), and plant cell cultures can be continuously carried out without seasonal constraints. According to Ribeiro et al (2015a), the use of biotransformation on plant cell culture systems has immense potential for production of compounds with commercial interest, especially considering the biochemical capability for production of secondary metabolites. So, biotransformation is also gaining considerable attention as a step towards green chemistry by reducing the usage of hazardous chemicals.…”