“…In the next step oxidative dimerization of selected substrates such as ferulic acid ( 1 ), eugenol ( 2 ) and isoeugenol ( 3 ) (Fig. 1) was studied since their dimers are well known for their biological activity as antioxidants, outdoing their respective monomers in certain cases ([8,12,13,[16], [17], [18], [19]]). These structures also play an important anti-inflammatory and chemopreventive roles.Fig.…”
Section: Resultsmentioning
confidence: 99%
“…3) showed less cytotoxicity and greater anti-inflammatory activity than its monomer 2 ([[25], [26], [27]]). Other authors claimed that eugenol and isoeugenol dimers, possess higher antitumoral activity [8], as well as promising use in antidiabetic drugs [15] and neuroprotective activity [13].…”
Section: Resultsmentioning
confidence: 99%
“…Compounds such as ortho-methoxy para -substituted phenols have attracted considerable attention due to its various biological and pharmacological activities, mainly as antioxidants [6,7]. Furthermore, some ortho-coupled dimer derivatives have been reported to present better biological properties than their monomeric counterparts [[8], [9], [10], [11], [12], [13], [14], [15]].…”
“…In the next step oxidative dimerization of selected substrates such as ferulic acid ( 1 ), eugenol ( 2 ) and isoeugenol ( 3 ) (Fig. 1) was studied since their dimers are well known for their biological activity as antioxidants, outdoing their respective monomers in certain cases ([8,12,13,[16], [17], [18], [19]]). These structures also play an important anti-inflammatory and chemopreventive roles.Fig.…”
Section: Resultsmentioning
confidence: 99%
“…3) showed less cytotoxicity and greater anti-inflammatory activity than its monomer 2 ([[25], [26], [27]]). Other authors claimed that eugenol and isoeugenol dimers, possess higher antitumoral activity [8], as well as promising use in antidiabetic drugs [15] and neuroprotective activity [13].…”
Section: Resultsmentioning
confidence: 99%
“…Compounds such as ortho-methoxy para -substituted phenols have attracted considerable attention due to its various biological and pharmacological activities, mainly as antioxidants [6,7]. Furthermore, some ortho-coupled dimer derivatives have been reported to present better biological properties than their monomeric counterparts [[8], [9], [10], [11], [12], [13], [14], [15]].…”
“…In fact, many plant peroxidases use ABTS as substrate, which in some cases is the main substrate for these enzymes [43,44]. In addition, one study showed that eugenol, an allyl chain-substituted guaiacol, can be oxidized by some peroxidases, although this reaction can result in the formation of cytotoxicity products [45]. When other chemicals were used as substrates (ascorbic acid, tyrosine, Coomassie brilliant blue, tryptophan, aminoantipyrine, NADH and syringaldazine), there was no detectable activity of MoPOX (Fig.…”
Peroxidases are ubiquitous enzymes involved in the oxidation of a variety of aromatic substrates including textile dyes, which are harmful for aquatic life and human health. Hence, the present study describes the purification of a peroxidase, named MoPOX, from Moringa oleifera roots using DEAE-Sephacel and gel filtration chromatography on a Superdex® 75 column. The peptide sequences recorded by mass spectrometry analysis confirmed the identity of MoPOX with other plant peroxidases. The optimum pH and temperature of enzyme activity were 5.2 and 70 °C, respectively. Its enzymatic activity in the presence of metal ions and classical peroxidase inhibitors was also evaluated. MoPOX follows Michaelis-Menten kinetics, with specificity, in ascending order, to the substrates ABTS < eugenol < O-dianisidine ≅ guaiacol, besides being highly thermostable. The purified peroxidase (0.015, 0.030 or 0.150 mg/mL) degraded different dyes (50 or 100 mg/L), such as Remazol ® Blue RGB, Remazol ® Navy RGB and Telon ® Turquoise M-5G 85%. Decolorization rates varied from 15 to 90% depending on the dye concentration, enzyme concentration and exposure time. MoPOX is the first peroxidase purified from M. oleifera roots, and the results showed it has biotechnological potential for biodegradation of hazardous compounds.
“…Eugenol also has antioxidant properties [5,6]. Another study showed that eugenol has anticancer activity [7,8]. Eugenol also has antidepressant and anthelminthic activities [9,10].…”
Objective: The main objective of this study was to determine the optimal ethanol concentration as solvent for extracting eugenol from galangal.Methods: Galangal rhizome extraction was conducted with kinetic maceration at 50°C with various ethanol concentration as solvent (0%, 30%, 50%, 70%, and 96%). A 1:10 ratio of rhizome and ethanol was applied. The extract was obtained, then its eugenol concentration was analyzed qualitatively and quantitatively by thin-layer chromatography-densitometer with n-hexane:ethyl acetate (4:1) by using mobile phase and silica gel 60 F 254 as stationary phase and applying a wavelength of 283 nm.
Results:The result of qualitative analysis showed that extract of 70% and 96% ethanol had a spot with Rf value 0.63, which was equal to the Rf value of standard eugenol. Standard curve equation for the 70% ethanol extract was y=89318x+656.07 (r=0.9993) and for the 96% ethanol extract was y=8658x+1743 (r=0.9999). The result of the quantitative analysis showed that the 70% and 96% ethanol extract contained 4.85% and 4.79% eugenol, respectively.
Conclusion:Extraction of galangal rhizome in 70% and 96% ethanol was positively containing eugenol. Highest eugenol concentration (4.85%) was obtained from galangal rhizome extraction in 70% ethanol.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
