Optimation of Ethanol Concentration in Extraction of Eugenol From Galangal Rhizome

Abstract

Objective: The main objective of this study was to determine the optimal ethanol concentration as solvent for extracting eugenol from galangal.Methods: Galangal rhizome extraction was conducted with kinetic maceration at 50°C with various ethanol concentration as solvent (0%, 30%, 50%, 70%, and 96%). A 1:10 ratio of rhizome and ethanol was applied. The extract was obtained, then its eugenol concentration was analyzed qualitatively and quantitatively by thin-layer chromatography-densitometer with n-hexane:ethyl acetate (4:1) by using mobile phase and silica gel 60 F 254 as stationary phase and applying a wavelength of 283 nm. Results:The result of qualitative analysis showed that extract of 70% and 96% ethanol had a spot with Rf value 0.63, which was equal to the Rf value of standard eugenol. Standard curve equation for the 70% ethanol extract was y=89318x+656.07 (r=0.9993) and for the 96% ethanol extract was y=8658x+1743 (r=0.9999). The result of the quantitative analysis showed that the 70% and 96% ethanol extract contained 4.85% and 4.79% eugenol, respectively. Conclusion:Extraction of galangal rhizome in 70% and 96% ethanol was positively containing eugenol. Highest eugenol concentration (4.85%) was obtained from galangal rhizome extraction in 70% ethanol.

Development of an efficient micropropagation system for Dioscorea bulbifera L. and phytochemical profile of regenerated plants

“…TLC profile of methanol extracts showed remarkable results that gives the clue for the presence of various phytochemicals. In the literature data, it has been reported that solvent system hexane:ethyl acetate (different ratios) has been successfully utilized for the better separation of phytochemicals in various plant species like Euphorbia pulcherrima [ 36 ] and Alpinia galangal [ 46 ].…”