Biotransformation of eugenol by suspension cultures of transgenic crown galls of Panax quinquefolium and suspension cultures of Nicotiana tabacum

Abstract

The biocatalytic ability of transgenic crown galls of Panax quinquefolium was evaluated by using eugenol (1) as a substrate and suspension cultures of Nicotiana tabacum as control system. Three biotransformed products, namely: 2-methoxy-4-(2-propenyl)phenyl-O-β-D-glucopyranoside (2, 67.11%), 2-methoxy-4-(2-propenyl)phenyl-O-β-D-glucopyranosyl (6' → 1″)-β-D-xylopyranoside (3, 2.85%) and methyl eugenol (4, 14.30%) were obtained after 5 days of administration of eugenol to the suspension cultures of transgenic crown galls of P. quinquefolium. In contrast, only one product, compound 2 (15.41%), was obtained in suspension cultures of N. tabacum after 5 days of incubation. The results indicated that the glycosylation ability of transgenic crown galls of P. quinquefolium was much higher than that of the cultured cells of N. tabacum.

Biotransformation of Licochalcone A by transgenic crown galls of Panax quinquefolium

“…Usually, the system of transgenic crown galls of panax quinquefolium was reported to use for the glycosylation of substrate, including the biotransformation of phenolic acids [19], anthraquinone [20], sesquiterpenes [21]. For example, in the biotransformation system of crown galls of P. quinquefolium: paeonol was monoglucosylated to yield paeonol-2-O-β-D-glucopyranoside [20].…”

“…The crown galls cultures, as a new form of culture system, possesses distinct advantages when compared with callus and cell culture, such as faster growth cycle, higher yield of transformed constituents, more secondary metabolites and lower contamination of exogenous phytohormones [7] Thus, crown galls can be applied to produce useful secondary metabolites efficiently and effectively.…”