Biotransformation of cholesterol and 16,17‐alpha epoxypregnenolone by novel <i>Cladosporium</i> sp. strain IS547

Pang, Cuiping; Cao, Yuting; Zhu, Xiangdong

doi:10.1002/jobm.201600191

Cited by 6 publications

(3 citation statements)

References 26 publications

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“…In this investigation, we used cholesterol as the sole carbon source to screen microbial transformation strains and obtained microorganisms with specific transformation functions that can convert cholesterol into 7-hydroxycholesterol [16]. The strains screened were identified and found to be Serratia via colony morphology observations, biochemical experiments and 16S rDNA sequence analysis.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of a Novel Depsipeptide and Prodigiosine of Serratia marcescans S823

Zhu¹,

Pang²,

Chen³

et al. 2018

Nat Prod Chem Res

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To investigate the antimicrobial activity of secondary metabolites from Serratia marcescans S823. The strain Serratia marcescans S823 was screened for its antibacterial activity. The active components from the bacterium were isolated by bioactivity-guided analysis. Their structures were identified by chemical and spectroscopic methods to be prodigiosine (PG) (1), serratamolide A (2) and serratamolide G (3), which is a new compound. The MIC and MBC of prodigiosine and serratamolide G were 6.25 μg.mL-1 and 12.5 μg.mL-1 against C. albicans and 6.25 μg.mL-1 and 25 μg.mL-1 against B. thuringiensis respectively. They might be potential alternative treatment options for Candida albicans infection. This study encourages further investigation of natural products as new antibiotics.

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Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of a Novel Depsipeptide and Prodigiosine of Serratia marcescans S823

Zhu¹,

Pang²,

Chen³

et al. 2018

Nat Prod Chem Res

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“…Low agitation could decrease the dissolved oxygen in the medium, which made it difficult to satisfy the strain needs for the oxygen. High agitation could increase the mechanical damage on the cell, which directly affected the growth of the cells [ 46 ]. The trans -dihydrocarvone content increased first and then decreased with the increase of agitation speed (100–300 rpm) in this study ( Figure 8 B).…”

Section: Resultsmentioning

confidence: 99%

Screening a Strain of Klebsiella sp. O852 and the Optimization of Fermentation Conditions for Trans-Dihydrocarvone Production

et al. 2021

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Flavors and fragrances have high commercial value in the food, cosmetic, chemical and pharmaceutical industries. It is interesting to investigate the isolation and characterization of new microorganisms with the ability to produce flavor compounds. In this study, a new strain of Klebsiella sp. O852 (accession number CCTCC M2020509) was isolated from decayed navel orange (Citrus sinensis (L.) Osbeck), which was proved to be capable of converting limonene to trans-dihydrocarvone. Besides, the optimization of various reaction parameters to enhance the trans-dihydrocarvone production in shake flask was performed for Klebsiella sp. O852. The results showed that the yield of trans-dihydrocarvone reached up to 1 058 mg/L when Klebsiella sp. O852 was incubated using LB-M medium for 4 h at 36 °C and 150 rpm, and the biotransformation process was monitored for 36 h after adding 1680 mg/L limonene/ethanol (final ethanol concentration of 0.8% (v/v)). The content of trans-dihydrocarvone increased 16 times after optimization. This study provided a basis and reference for producing trans-dihydrocarvone by biotransformation.

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“…Dexamethasone is usually classified as a steroid‐derived metabolic active drug. The metabolic production/conversion of this compound class can be catalyzed by gut microbiota bacteria and it exhibits an action vs Clostridium difficile . Basically, it inhibits the biological activity of toxin B of Clostridium difficile .…”

Section: Molecular Fingerprint Potentially Produced By Gut Microbiotamentioning

confidence: 99%

Predicting and preventing intestinal dysbiosis on the basis of pharmacological gut microbiota metabolism

Cristoni

Bernardi

Larini

et al. 2019

Rapid Comm Mass Spectrometry

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The possible relationship(s) between intestinal dysbiosis and various degenerative intestinal-based diseases, e.g. diabetes and obesity, is an ongoing focus of intense investigation. [1][2][3][4] As a consequence of the results of such research, the focus is now on therapeutic strategies for such diseases based on modification of the gut microbiota. 2,4 Fecal transplantation is one such strategy, which was suggested by Zhang and co-workers 2 as a treatment for diabetes mellitus and by Kang and co-workers 4 for treatment of obesity. Kang and co-workers also reported different strategies involving fecal gut microbiota transplantation to treat obesity. Other studies correlated other degenerative diseases to gut microbiota activities and suggested therapeutic strategies to alleviate those disease states. 1 This interesting prospect, i.e., fecal gut microbiota transplantation to combat intestine-related diseases, has led to the development of methods by different research groups to evaluate the gut microbiota status in human subjects. 5-8 These approaches can be classified as:a. Untargeted approaches that search for bacteria responsible for dysbiosis without any prior knowledge of which types of bacterium might be involved. 5,6 b. Targeted approaches that search for specific pathogens and the correlation of these to gut microbiota-related dysbiosis. 7,8 One example of an untargeted approach is that of Pinheiro de Oliveira and co-workers who developed a method that detects thousands of different species using DNA gene amplification. 5 For the aforementioned DNA approaches, it is difficult to determine a correlation between the presence of a bacterial species and its involvement in intestinal dysbiosis without knowledge of how its metabolism affects the intestinal environment. 5,6

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Biotransformation of cholesterol and 16,17‐alpha epoxypregnenolone by novel Cladosporium sp. strain IS547

Cited by 6 publications

References 26 publications

Antibacterial Activity of a Novel Depsipeptide and Prodigiosine of Serratia marcescans S823

Antibacterial Activity of a Novel Depsipeptide and Prodigiosine of Serratia marcescans S823

Screening a Strain of Klebsiella sp. O852 and the Optimization of Fermentation Conditions for Trans-Dihydrocarvone Production

Predicting and preventing intestinal dysbiosis on the basis of pharmacological gut microbiota metabolism

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