Biotransformation of artemisinic acid by the fungus <i>Trichothecium roseum</i> and anti-candidal activity of its metabolites

Singh, Pooja; Kapkoti, Deepak Singh; Singh, Nandan; Tewari, Rashmi; Saikia, Dharmendra; Rout, Prasant Kumar; Pandey, Rakesh; Bhakuni, R. S.

doi:10.1080/10242422.2018.1552265

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…Several Trichothecium sp species produce trichothecinol-A, which has antifungal, anticancer, and antimetastatic effects (Taware et al, 2014). The compounds 3hydroxyartemisinic acid, 3,15-dihydroxyartemisinic acid, and 3-Oxoartemisinic acid produced by the biotransformation of T. roseum's artemisinic acid have antifungal properties as well (Singh et al, 2019). Additionally, it produces cuspidatol compounds belonging to the sesquiterpene group, trichomide cyclodepsopeptides which exhibit immunosuppressive activity (Zhou et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Bioactivity of Endophytic Fungi Isolated from Branch of Jambu Mawar (Syzygium jambos (L.) Alston)

Aini

Elfita

Widjajanti

et al. 2023

molekul

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Endophytic fungi isolated from medicinal plants have high diversity and the secondary metabolites produced have biological activity. Endophytic fungi isolated from medicinal plants have been the subject of many studies on their diversity and biological activity, one of which is jambu mawar (Syzygium jambos (L.) Alston). S. jambos has long been used as a traditional medicine to treat infections caused by pathogenic bacteria in many parts of the world, including South Sumatra. This study purposed to explore the diversity of endophytic fungi isolated from the branches of S. jambos, the antibacterial activity of endophytic fungi extracts, the determination of the structure of the compounds, and the activity of the active compounds of the selected endophytic fungi. Observations of the morphological characteristics of endophytic fungi were macroscopically and microscopically. The endophytic fungal extracts were then tested for antimicrobial activity against test bacteria Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, and Escherichia coli using the Kirby-Bauer paper disk diffusion method. Isolation of pure compounds using a gravity chromatography column, the determination of the structure of the compound is determined based on 1H-NMR, 13C-NMR, HMQC, and HMBC spectroscopy. Four endophytic fungi, code SJC1–4, were isolated from a branch of S. jambos. The results of macroscopic and microscopic morphological characterization showed three genera of Botryosphaeria, Trichothecium, and Aspergillus. The endophytic fungal isolate SJC1 exhibited strong activity against Gram-positive bacteria and moderate activity against Gram-negative bacteria, while SJC2–4 showed moderate activity against the four bacteria. Molecular identification of SJC1 revealed that it was Botryosphaeria mamane. Isolation of SJC1’s pure compound yielded compound 1, which was identified as 5-acetyl-6-hydroxy-3-methyl-2H-pyran-2-one. This compound is thought to have antibacterial properties.

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Section: Resultsmentioning

confidence: 99%

Bioactivity of Endophytic Fungi Isolated from Branch of Jambu Mawar (Syzygium jambos (L.) Alston)

Aini

Elfita

Widjajanti

et al. 2023

molekul

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“…Also, the global demand for by-products has steadily gained momentum in the current prevailing conditions of pandemic outbreak for the conversion of macromolecules into biomedicine. Singh et al (2019) reported the potential of cell culture of endophytic fungus Trichothecium roseum CIMAPN in transforming a pharmaceutical molecule, artemisinic acid, into a better derivative (3-oxoartemisinic acid) with anti-candidal potential.…”

Section: Applications Of Endophytic Fungi As Biocatalystmentioning

confidence: 99%

Endophytic Fungi-Mediated Biocatalysis and Biotransformations Paving the Way Toward Green Chemistry

Choudhary

Gupta

Dhar

et al. 2021

Front. Bioeng. Biotechnol.

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Catalysis is a process carried out in the presence of a heterogenous catalyst for accelerating the rate of a chemical reaction. It plays a pivotal role in transition from take, make, and dispose technology to sustainable technology via chemo- and biocatalytic processes. However, chemocatalyzed reactions are usually associated with copious amounts of perilous/hazardous environmental footprints. Therefore, whole-cell biotransformations or enzyme cocktails serve as cleaner biocatalytic alternatives in replacing the classical chemical procedures. These benchmark bioconversion reactions serve as important key technology in achieving the goals of green chemistry by eliminating waste generation at source. For this, nature has always been a driving force in fuelling natural product discovery and related applications. The fungal endophytic community, in particular, has undergone co-evolution with their host plant and has emerged as a powerful tool of genetic diversity. They can serve as a treasure trove of biocatalysts, catalyzing organic transformations of a wide range of substances into enantiopure compounds with biotechnological relevance. Additionally, the biocatalytic potential of endophytic fungi as whole-intact organisms/isolated enzyme systems has been greatly expanded beyond the existing boundaries with the advancement in high-throughput screening, molecular biology techniques, metabolic engineering, and protein engineering. Therefore, the present review illustrates the promising applications of endophytic fungi as biocatalysts for the synthesis of new structural analogs and pharmaceutical intermediates and refinement of existing proteins for novel chemistries.

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Global scenario of endophytic fungal research: Biodiversity and biotechnological applications

Khan,

Rasool,

Kour

et al. 2024

Endophytic Fungi

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Biotransformation of artemisinic acid by the fungus Trichothecium roseum and anti-candidal activity of its metabolites

Cited by 3 publications

References 26 publications

Bioactivity of Endophytic Fungi Isolated from Branch of Jambu Mawar (Syzygium jambos (L.) Alston)

Bioactivity of Endophytic Fungi Isolated from Branch of Jambu Mawar (Syzygium jambos (L.) Alston)

Endophytic Fungi-Mediated Biocatalysis and Biotransformations Paving the Way Toward Green Chemistry

Global scenario of endophytic fungal research: Biodiversity and biotechnological applications

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