Biotoxin sensing in food and environment via microchip

Zhang, Zhaowei; Yu, Li; Xu, Lin; Hu, Xiaofeng; Li, Peiwu; Zhang, Qi; Ding, Xiaoxia; Feng, Xiaojun

doi:10.1002/elps.201300570

Cited by 33 publications

(18 citation statements)

References 106 publications

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“…It can readily contaminate both food and feed during almost all stages, e.g., before and after harvest and during storage, transportation, and consumption. It has been recognized that the fungal- or mycotoxin-induced diseases increasingly threaten both biodiversity and food security [ 1 ]. Recently, it is indicated that a fungal threat from AFB 1 and other mycotoxins (or fungus) could cause a 39% loss in the world's food supply if severe epidemics were to occur simultaneously on all five crops [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid On-Site Sensing Aflatoxin B1 in Food and Feed via a Chromatographic Time-Resolved Fluoroimmunoassay

et al. 2015

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Aflatoxin B1 poses grave threats to food and feed safety due to its strong carcinogenesis and toxicity, thus requiring ultrasensitive rapid on-site determination. Herein, a portable immunosensor based on chromatographic time-resolved fluoroimmunoassay was developed for sensitive and on-site determination of aflatoxin B1 in food and feed samples. Chromatographic time-resolved fluoroimmunoassay offered a magnified positive signal and low signal-to-noise ratio in time-resolved mode due to the absence of noise interference caused by excitation light sources. Compared with the immunosensing performance in previous studies, this platform demonstrated a wider dynamic range of 0.2-60 μg/kg, lower limit of detection from 0.06 to 0.12 µg/kg, and considerable recovery from 80.5% to 116.7% for different food and feed sample matrices. It was found to be little cross-reactivity with other aflatoxins (B2, G1, G2, and M1). In the case of determination of aflatoxin B1 in peanuts, corn, soy sauce, vegetable oil, and mouse feed, excellent agreement was found when compared with aflatoxin B1 determination via the conversational high-performance liquid chromatography method. The chromatographic time-resolved fluoroimmunoassay affords a powerful alternative for rapid on-site determination of aflatoxin B1 and holds a promise for food safety in consideration of practical food safety and environmental monitoring.

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Section: Introductionmentioning

confidence: 99%

Rapid On-Site Sensing Aflatoxin B1 in Food and Feed via a Chromatographic Time-Resolved Fluoroimmunoassay

et al. 2015

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“…www.nature.com/scientificreports www.nature.com/scientificreports/ relevance in remote areas, and resource limited developing countries like India due to its stability, ease of production, and use 20 . To meet this requirement, aptamer (as a biorecognition element) based detection has recently increased in demand, due to its higher specificity and lesser cross reactivity as compared to antibodies, as well as ease of production 12,21 .…”

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confidence: 99%

Fabrication of microfluidic device for Aflatoxin M1 detection in milk samples with specific aptamers

Kasoju

Shahdeo

Khan

et al. 2020

Sci Rep

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This study describes the colorimetric detection of aflatoxin M1 (Afl M1) in milk samples using a microfluidic paper-based analytical device (µPAD). Fabrication of µPADs was done using a simple and quick approach. Each μPAD contained a detection zone and a sample zone interconnected by microchannels. The colorimetric assay was developed using unmodified AuNPs as a probe and 21-mer aptamer as a recognition molecule. The free aptamers were adsorbed onto the surface of AuNPs in absence of Afl M1, even at high salt concentrations. The salt induced aggregation of specific aptamers occurred in presence of Afl M1. Under optimum conditions, the analytical linear range was found to be 1 µM to 1 pM with limit of detection 3 pM and 10 nM in standard buffer and spiked milk samples respectively. The proposed aptamer based colorimetric assay was repeatable, quick, selective, and can be used for on-site detection of other toxins in milk and meat samples. Mycotoxins are secondary metabolites produced by filamentous fungi belonging to the genera Aspergillus and Penicillium 1. Mycotoxins are also found in animal derived foods such as milk due to intake of contaminated feed 2,3. Considering agricultural and economical aspects and possible implications on public health, the most relevant mycotoxins are aflatoxins, ochratoxins, fuminosins, T-2 toxin, and zearalenone (ZEA) 4. Aflatoxins pose a huge economic burden as they cause around 25% or more loss of the world's food crops every year 5. Afl M1 (4-hydroxy aflatoxin B1) and M2 (4-dihydroxy aflatoxin B1) have well proven carcinogenic and mutagenic potentiality and pose severe health consequences on milk consumers, including the risk of cancer and stunted growth in children below the age of 5 years 6. When B1 is ingested by a cow, it is secreted as hydroxylated metabolite aflatoxin M1 (Afl M1) in the urine and milk of the cow 7. Consumption of food containing aflatoxin concentrations of one milligram/kilogram or higher has been suspected to cause aflatoxicosis 8 , the prognosis of which consists of acute liver failure, jaundice, lethargy, and nausea, eventually leading to death within 1 to 2 weeks, based on past reports 9. Thus there is a need to develop rapid low cost technology based highly specific methods for detection of aflatoxins to improve surveillance and control in rural areas. A plethora of analytical techniques are available for aflatoxin M1 detection, ranging from chromatography and HPLC-MS used for regulatory control in official laboratories to rapid test kits for grain silos and farmers, especially for surveys when outbreaks occur 10,11. Conventional techniques for the detection of aflatoxins include gas chromatography (GC), fluorescence or UV based detection, thin layer chromatography (TLC) and enzyme linked immunosorbent assay (ELISA) 6-12. These techniques are routinely used and yield reliable results, however they are expensive, time consuming, require large scale instrumentation and large amounts of hazardous chemical reagents. Advancement in the development of bios...

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“…Microfluidics is an emerging technology designed for the controlled movement of small fluid volumes through a microchannel network [ 18 , 19 ]. In principle, microfluidic platforms offer the potential for a portable, simple-to-use, and inexpensive lab-on-chip device for rapid multi-analyte detection of small volume samples [ 20 , 21 , 22 ]. Indeed, a multitude of customized microfluidic solutions have been shown effective for analyte detection as R&D tools, but few have translated into successful commercial products adopted by end-users [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Rapid Microfluidic Assay for the Detection of Botulinum Neurotoxin in Animal Sera

Babrak

Lin

Stanker

et al. 2016

Toxins

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Potent Botulinum neurotoxins (BoNTs) represent a threat to public health and safety. Botulism is a disease caused by BoNT intoxication that results in muscle paralysis that can be fatal. Sensitive assays capable of detecting BoNTs from different substrates and settings are essential to limit foodborne contamination and morbidity. In this report, we describe a rapid 96-well microfluidic double sandwich immunoassay for the sensitive detection of BoNT-A from animal sera. This BoNT microfluidic assay requires only 5 μL of serum, provides results in 75 min using a standard fluorescence microplate reader and generates minimal hazardous waste. The assay has a <30 pg·mL−1 limit of detection (LOD) of BoNT-A from spiked human serum. This sensitive microfluidic BoNT-A assay offers a fast and simplified workflow suitable for the detection of BoNT-A from serum samples of limited volume in most laboratory settings.

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Biotoxin sensing in food and environment via microchip

Cited by 33 publications

References 106 publications

Rapid On-Site Sensing Aflatoxin B1 in Food and Feed via a Chromatographic Time-Resolved Fluoroimmunoassay

Rapid On-Site Sensing Aflatoxin B1 in Food and Feed via a Chromatographic Time-Resolved Fluoroimmunoassay

Fabrication of microfluidic device for Aflatoxin M1 detection in milk samples with specific aptamers

Rapid Microfluidic Assay for the Detection of Botulinum Neurotoxin in Animal Sera

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