Rapid On-Site Sensing Aflatoxin B1 in Food and Feed via a Chromatographic Time-Resolved Fluoroimmunoassay

Abstract

Aflatoxin B1 poses grave threats to food and feed safety due to its strong carcinogenesis and toxicity, thus requiring ultrasensitive rapid on-site determination. Herein, a portable immunosensor based on chromatographic time-resolved fluoroimmunoassay was developed for sensitive and on-site determination of aflatoxin B1 in food and feed samples. Chromatographic time-resolved fluoroimmunoassay offered a magnified positive signal and low signal-to-noise ratio in time-resolved mode due to the absence of noise interference caused by excitation light sources. Compared with the immunosensing performance in previous studies, this platform demonstrated a wider dynamic range of 0.2-60 μg/kg, lower limit of detection from 0.06 to 0.12 µg/kg, and considerable recovery from 80.5% to 116.7% for different food and feed sample matrices. It was found to be little cross-reactivity with other aflatoxins (B2, G1, G2, and M1). In the case of determination of aflatoxin B1 in peanuts, corn, soy sauce, vegetable oil, and mouse feed, excellent agreement was found when compared with aflatoxin B1 determination via the conversational high-performance liquid chromatography method. The chromatographic time-resolved fluoroimmunoassay affords a powerful alternative for rapid on-site determination of aflatoxin B1 and holds a promise for food safety in consideration of practical food safety and environmental monitoring.

An On-Site, Ultra-Sensitive, Quantitative Sensing Method for the Determination of Total Aflatoxin in Peanut and Rice Based on Quantum Dot Nanobeads Strip

“…The standard curve, LOD, linear range, and specificity were estimated as described in our previous report [17]. For standard curves and LOD on a QDNBs-based ICA strip, the fluorescence intensities of the T and C lines were used for sensitive and quantitative detection.…”

“…Typically, the gold nanoparticle has been substantiated as an effective reporter for colorimetric detection, allowing qualitative determination for mycotoxin with relatively high concentrations [6,10,11,12]. Furthermore, to meet the requirements of detection of sensitive mycotoxins such as AFT, some quantitative test strip methods have been developed recently using various reporters such as liposome-encapsulated dyes [13], magnetic nanogold microspheres [14],time-resolved fluorescence [15,16,17], up-converting phosphor [18], fluorescent microspheres [19], and quantum dots (QDs) [20,21,22]. Owing to the intrinsically high sensitivity, the fluorescence-based strip could qualify application in food safety [9].…”

A Focus on Aflatoxin in Feedstuffs: New Developments in Analysis and Detection, Feed Composition Affecting Toxin Contamination, and Interdisciplinary Approaches to Mitigate It

“…The toxin was revealed using a 366 nm wavelength. Finally, Zhang and coworkers developed an on-site analysis for aflatoxin B 1 in food and feed samples using a chromatographic time-resolved fluoroimmunoassay that offered a magnified positive signal and low signal-to-noise ratio [110]. Wang and coworkers developed a fluorescence based on europium nanospheres and monoclonal antibodies for the determination of total aflatoxin in the feed with a 0.16 μg kg −1 limit of detection [111].…”

A Portable Liquid Chromatograph with a Battery-operated Compact Electroosmotic Pump and a Microfluidic Chip Device with a Reversed Phase Packed Column

“…We believe there is a potential need for on-site application of HPLC systems in various fields such as product development, plant management, quality control, food analysis, 1 wastewater management, environmental investigation, [2][3][4] forensic analysis, clinical diagnosis, veterinary science, and sports science. This wide array of applications suggests both indoor and outdoor uses for HPLC systems.…”