Biotin-Containing Reduced Graphene Oxide-Based Nanosystem as a Multieffect Anticancer Agent: Combining Hyperthermia with Targeted Chemotherapy

Mauro, Nicolò; Scialabba, Cinzia; Cavallaro, Gennara; Licciardi, Mariano; Giammona, Gaetano

doi:10.1021/acs.biomac.5b00705

Cited by 52 publications

(50 citation statements)

References 29 publications

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“…15, 16 We provided evidence of enhanced internalization of this system in cancer cells instead of the normal counterpart thanks to the conjugate's ability of releasing doxorubicin and reversing its net charge, from negative to positive, once the cancer site is reached, thus improving the preferential release of the drug at the site of action and throughout the inside of cells. This outcome was accomplished by exploiting a pH-sensitive cleavage mechanism triggered by the acidic pH of cancer cells (6.4 < pH < 5.5).…”

Section: 4-6mentioning

confidence: 91%

Polyaminoacid–doxorubicin prodrug micelles as highly selective therapeutics for targeted cancer therapy

et al. 2016

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An amphiphilic copolymer carrying high-dose doxorubicin (21% on a weight basis), PHEA-EDA-P,C-Doxo, was prepared by coupling doxorubicin with a biocompatible polyaminoacid through a pH-sensitive spacer.Additional derivatization with 4-pentynoic acid endows it with self-assembling properties by means of p-p stacking. These micelles can be triggered to promptly release drug in lysosomes ($40% in 12 h) through pHdependent micelle hydrolysis after uptake. In vitro tests on co-cultures of cancer (MDA-MB 231) and normal (HB-2) breast cells proved that the conjugate was selectively internalized into the former rather than normal cells, exploiting the caveolae-dependent endocytosis pathway, explaining the selective cytotoxic effect toward cancer cells. Intracellular trafficking study of MDA-MB 231 showed that the delivery of the endocytosed drug occurs through the direct fusion of caveosomes with late lysosomes, triggering a massive release in the cytoplasm, bringing about cell death. Dose-effectiveness and mechanistic data indicate that PHEA-EDA-P,C-Doxo is endowed with a distinctive combination of selectivity and pharmacological potency (EC50 13 mM, E max ¼ 77% and EC50 > 25 mM, E max ¼ 21% for cancer and healthy cells respectively) that makes it an excellent candidate for future preclinical studies.

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Section: 4-6mentioning

confidence: 91%

Polyaminoacid–doxorubicin prodrug micelles as highly selective therapeutics for targeted cancer therapy

et al. 2016

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“…Multifunctional biocompatible GO quantum dots decorated magnetic nanoplatform were utilized for efficient capture and two-photon imaging of rare tumor cells 235 . Demeritte et al 236 reported hybrid GO based plasmonic-magnetic multifunctional nanoplatform for selective separation and label-free identification of alzheimer's disease biomarkers biotin containing rGO based nano system was proved as a multi-effect anticancer agent by Mauro et al 237 . The hybrid based on rGO with ultrasmall gold nanorod were utilized for a sequential drug release and enhanced photothermal and photoacoustic effect for cancer therapy 238 .…”

Section: Bio Applicationsmentioning

confidence: 99%

Graphene oxide: strategies for synthesis, reduction and frontier applications

2016

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Till now, several innovative methods have been developed for the synthesis of graphene materials including mechanical exfoliation, epitaxial growth by chemical vapor deposition, chemical reduction of graphite oxide, liquid-phase exfoliation, arc discharge of graphite, in situ electron beam irradiation, epitaxial growth on SiC, thermal fusion, laser reduction of polymers sheets and unzipping of carbon nanotubes etc. Generally large scale graphene nanosheets are reliably synthesized utilising other forms of graphene-based novel materials, including graphene oxide (GO), exfoliated graphite oxide (by thermal and microwave), and reduced graphene oxide. The degree of GO reduction and number of graphene layers are minimized mainly by applying two approaches via chemical or thermal treatments. The promising and excellent properties together with the ease of processibility and chemical functionalization makes graphene based materials specially GO, ideal candidates for incorporation into a variety of advanced functional materials. Chemical functionalization of graphene can be easily achieved, by the introduction of various functional groups. These functional groups help to control and manipulate the graphene surfaces and help to tune the properties of the resulting hybrid materials. Importantly, graphene and its derivatives GO, have been explored in a wide range of applications, such as energy generation/storage, optical devices, electronic and photonic devices, drug delivery, clean energy, and chemical/bio sensors. In this review article, we have incorporated general introduction of GO, their synthesis, reduction and some selected frontier applications.

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“…After 1 min of irradiation, the NIR absorption peak of indocyanine green almost disappeared, whereas the peak of indocyanine green on rGO or hyaluronic acid-anchored rGO was retained even after 5 min of irradiation. In the study of Mauro et al, [ 116 ] in-vitro rGO-induced hyperthermia was assessed and combined with the stimuli-sensitive anticancer effect of a biotinylated inulin-doxorubicin conjugate, hence, getting a nanosystem endowed with synergic anticancer effects and high specifi city. In-vitro tests using cancer breast cells showed the ability of the bioconjugate to effi ciently kill cancer cells both via a selective laser beam thermo-ablation and hyperthermia-triggered chemotherapy.…”

Section: Photothermal Anticancer Therapymentioning

confidence: 99%

Recent Advances in Laser Utilization in the Chemical Modification of Graphene Oxide and Its Applications

Trusovas

Račiukaitis

Niaura

et al. 2015

Advanced Optical Materials

147

110

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the manipulation of graphene and its derivatives occupy a relatively insignifi cant part, i.e., less than 1%. The fi rst publications on graphene modifi cation with laser irradiation appeared in 2008, however, since 2010, the number of publications has risen exponentially.Graphene oxide (GO) has emerged as a versatile material for nanocarbon research. Several types of oxygen-containing functional groups present on the basal plane, and the sheet edge allows GO to interact with a broad range of organic and inorganic materials. [ 9 ] Furthermore, GO is an abundant and low-cost material synthesized from graphite or graphite oxide; it retains a layered structure but, at the same time, it can be characterized by the loss of electronic conjugation caused by the oxy groups at the edge or plane defects. GO has a potential use in energy, [ 10 ] electronics, [ 11 ] molecular sensing areas, [ 12 ] catalysis, [ 13 ] etc. Moreover, it can be reduced chemically or thermally in order to achieve graphenelike properties. [ 14 ] The material produced during such a procedure is usually referred to as "reduced GO" (rGO). However, residual defects such as remnant oxygen atoms, [ 15 ] Stone-Wales defects (pentagon-heptagon pairs), [ 16 ] and holes [ 17 ] appearing due to the loss of carbon (in the form of CO or CO 2 ) from the basal plane [ 18 ] limit the electronic quality of rGO.A laser is a powerful tool which is used for various applications in industry, medicine, science, and material processing. [ 19 ] It is usually employed in different processes such as ablation, etching, cutting of various materials, as well as direct A dramatic rise in research interest in laser-induced graphene oxide (GO) reduction and modifi cation requires an overview of the most recent works on this subject. Typical methods for the recognition and confi rmation of modifi ed graphene and its derivatives, such as Raman, Fourier-transform infra-red (FTIR), X-ray photoelectron (XP), and ultraviolet-visible (UV-vis) spectroscopies, are introduced briefl y in this review. A major part of the survey is devoted to the main modifi cation ways and the laser parameters used in the literature. A discussion of possible reduction and modifi cation mechanisms is also presented. Recent applications, especially in the biomedical fi eld such as cell therapy treatment, as well as signifi cant results of GO modifi cation, are discussed in detail. Finally, perspectives for the application of laser-induced GO modifi cations in passive THz photonics and biomedicine are briefl y addressed.

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Biotin-Containing Reduced Graphene Oxide-Based Nanosystem as a Multieffect Anticancer Agent: Combining Hyperthermia with Targeted Chemotherapy

Cited by 52 publications

References 29 publications

Polyaminoacid–doxorubicin prodrug micelles as highly selective therapeutics for targeted cancer therapy

Polyaminoacid–doxorubicin prodrug micelles as highly selective therapeutics for targeted cancer therapy

Graphene oxide: strategies for synthesis, reduction and frontier applications

Recent Advances in Laser Utilization in the Chemical Modification of Graphene Oxide and Its Applications

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