Biotechnology of oil palm: Current status of oil palm genetic transformation

Masani, Mat Yunus Abdul; Izawati, Abang Masli Dayang; Rasid, Omar Abdul; Parveez, Ghulam Kadir Ahmad

doi:10.1016/j.bcab.2018.07.008

Cited by 38 publications

(40 citation statements)

References 93 publications

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“…The availability of oil palm omics data, especially transcriptome analysis of various tissues and development stages has facilitated the identification and isolation of key genes, which are being selectively applied in research related to genetic modification of oil palm (Masani et al, 2018). The existing omics information also has huge potential for utilisation in the exciting area of genome editing.…”

Section: Omics -A Tool To Assist In the Generation And Assessment Of mentioning

confidence: 99%

Omics Platform Technologies for Discovery and Understanding the Systems Biology of Oil Palm

Ramli¹

2020

JOPR

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Palm oil is the leading vegetable oil in terms of volume in the world market. Indonesia and Malaysia are the top producers and exporters of the commodity. The global production of palm oil reached 73.5 million tonnes in the period 2018/2019, up from approximately 70.5 million tonnes in 2017/2018. Oil palm is the most productive crop in the world being 10 times more productive than soyabean which produces only about 0.45 t oil per hectare. Nonetheless, the industry is continuously under pressure to improve productivity and sustainability. This will require concerted innovations across the entire palm oil supply chain and fully committed research efforts including upstream technologies to expedite crop improvement. At the Malaysian Palm Oil Board (MPOB), research efforts at development of tools for improving oil palm traits by genetic modification have been augmented more recently with omics-based approaches and all of these innovations are synchronised towards improved product quality. Omics is a multidisciplinary field encompassing genomics, epigenomics, transcriptomics, proteomics and metabolomics. Each of these fields provides an opportunity to understand and view oil palm biology from a global perspective, enabling accelerated discoveries for improved productivity and the development of new and improved varieties. An integrative omics approach promises great value in both phenotyping and diagnostic analyses. With the current technological capabilities, metabolomics is also being exploited for identifying unique chemical fingerprints to detect product contamination and adulteration in oil palm. This effort is actively being conducted in order to position the oil palm industry to meet and optimise the delivery of the highest quality oil with minimum environmental and social concerns. In this review, an overview is given on the current knowledge and progress made in oil palm research, focusing on the application of omics strategies and their integration with high-throughput technologies for oil palm crop improvement, development of geographical traceability system and ensuring that palm oil is free from residual oil contamination.

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Section: Omics -A Tool To Assist In the Generation And Assessment Of mentioning

confidence: 99%

Omics Platform Technologies for Discovery and Understanding the Systems Biology of Oil Palm

Ramli¹

2020

JOPR

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“…MSM sebagai media penggorengan dapat digunakan namun suhu sebaiknya tidak lebih dari 150 °C karena senyawa karoten mudah terdegradasi selama pemanasan (Hasibuan et al, 2013;Masani et al, 2018;Ribeiro et al, 2018;Ricaurte et al, 2018). Oleh karena itu, MSM cocok sebagai media penggorengan untuk menumis sayur, sambal, ikan, dan lain-lain.…”

Section: Pendahuluanunclassified

Penggunaan Minyak Sawit Merah Dalam Pembuatan Sambal Cabai Merah Tumis

Hasibuan

Meilano²

2018

JTP

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Sambal cabai merah tumis dibuat dengan cara menumis cabai mentah giling menggunakan minyak goreng. Nutrisi sambal cabai merah ditingkatkan menggunakan minyak sawit merah (MSM) sebagai media penumisan karena mengandung fitonutrien berupa karoten (pro-vitamin A) dan tokoferol & tokotrienol (vitamin E). Penelitian ini mengkaji pengaruh rasio cabai dan minyak, pengaruh penambahan garam dan gula, pengaruh suhu dan waktu pemasakan, pengaruh jenis minyak, uji ketahanan simpan dan uji penerimaan dalam pembuatan sambal cabai merah menggunakan MSM dan fraksi oleinnya (MSMOL). Parameter mutu minyak yang dianalisis meliputi komposisi asam lemak, kadar air, kadar karoten, bilangan peroksida, dan kadar karoten. Analisa tingkat kesukaan produk sambal meliputi kenampakan warna, tekstur, aroma, dan rasa. Penerimaan terhadap kesukaan sambal tertinggi yaitu pada rasio cabai dan MSM atau MSMOL adalah 50:40 (g/g), rasio garam dan gula adalah 2% dan 4% terhadap berat cabai. Suhu dan waktu pemasakan mempengaruhi kadar air dan karoten sambal. Semakin tinggi suhu, menurunkan kadar air, namun meningkatkan kadar karoten pada sambal (kecuali pada suhu > 150 °C, kadar karoten menurun). Pemasakan sambal dengan mencampurkan cabai dengan MSM, kemudian dipanaskan secara bersamaan pada suhu 150 °C selama 5 menit. Tingkat penerimaan sambal cabai merah menggunakan MSM atau MSMOL relatif sama dengan minyak goreng komersial (MGK) sebagai kontrol. Sambal cabai merah menggunakan MSMOL lebih tahan lama dibandingkan MGK dan MSM ditinjau dari mutunya meliputi kadar asam lemak bebas, bilangan peroksida, dan kadar karoten. Waktu yang baik untuk menyimpan sambal cabai dengan MSMOL adalah 2 minggu pada 20-24 °C atau 1 minggu pada 28-30 °C

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“…Genetic engineering can be used to introduce novel genes to oil palm varieties and produce high-value traits that are almost impossible to be obtained through conventional breeding (Masani et al 2018). This technique may shorten the duration of oil palm breeding because the genetic modification can be applied directly to existing superior oil palm varieties and do not require backcross processes.…”

Section: Introductionmentioning

confidence: 99%

Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis)

Darmawan¹,

Wiendi

Utomo³

et al. 2020

Biodiversitas

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Abstract. Darmawan C, Wiendi NMA, Utomo C, Liwang T. 2020. Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis). Biodiversitas 21: 3720-3726. Novel traits introduction to existing varieties may shorten the duration of oil palm genetic improvement. This approach heavily relies on efficient plant transformation and regeneration methods. Commonly used methods such as biolistic and Agrobacterium-mediated transformation still generated low efficiency for oil palm. Therefore, it is important to find alternative transformation methods that have the potency to give better results. Electroporation is usually used for delivering genes into bacteria or plant protoplast where cell wall does not exist, however, in this research, it was used to deliver plasmid into plant tissues. The aim of this research is to establish an optimum electroporation protocol for delivering genes into oil palm calli. Electric field strength of 250, 500, 750, 1,000, and 1,250 V/cm were applied for oil palm calli electroporation. Transient GUS assay was not applicable for initial detection presumably due to oil palm endogenous GUS-like protein activity after electroporation. Delivered gene could be detected in survived calli from all tested electric field strength treatments and the highest calli growth rates were observed from 250 V/cm electroporation treatment. Overall results showed that electroporation could be used to deliver specific genes into oil palm calli and might be developed further to increase its efficiency.

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Biotechnology of oil palm: Current status of oil palm genetic transformation

Cited by 38 publications

References 93 publications

Omics Platform Technologies for Discovery and Understanding the Systems Biology of Oil Palm

Omics Platform Technologies for Discovery and Understanding the Systems Biology of Oil Palm

Penggunaan Minyak Sawit Merah Dalam Pembuatan Sambal Cabai Merah Tumis

Electroporation-mediated genetic transformation of oil palm (Elaeis guineensis)

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