Biotechnology and Bioinformatics of Endophytes in Biocontrol, Bioremediation, and Plant Growth Promotion

Slama, Houda Ben; Cherif‐Silini, Hafsa; Bouket, Ali Chenari; Silini, Allaoua; Alenezi, Faizah N.; Luptáková, Lenka; Vallat, Armelle; Belbahri, Lassaâd

doi:10.1007/978-3-030-65447-4_8

Cited by 7 publications

(11 citation statements)

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“…Extreme climatic conditions can also result in disease, insect, and pathogen infestations, and these biotic stressors have substantial impact on the quality and quantity of secondary metabolites produced by medicinal plants. Pathogenic microbes in medicinal plants induce infection and offer a significant risk to patients ( Chadha et al., 2015 ; Slama et al., 2021 ). However, controlling some plant pathogenic diseases such as vascular wilt is challenging ( Yadeta and Thomma, 2013 ).…”

Section: Endophytes Intervention Towards Improving Limitations Associ...mentioning

confidence: 99%

Contribution of endophytes towards improving plant bioactive metabolites: a rescue option against red-taping of medicinal plants

Tsipinana,

Husseiny,

Alayande

et al. 2023

Front. Plant Sci.

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Medicinal plants remain a valuable source for natural drug bioprospecting owing to their multi-target spectrum. However, their use as raw materials for novel drug synthesis has been greatly limited by unsustainable harvesting leading to decimation of their wild populations coupled with inherent low concentrations of constituent secondary metabolites per unit mass. Thus, adding value to the medicinal plants research dynamics calls for adequate attention. In light of this, medicinal plants harbour endophytes which are believed to be contributing towards the host plant survival and bioactive metabolites through series of physiological interference. Stimulating secondary metabolite production in medicinal plants by using endophytes as plant growth regulators has been demonstrated to be one of the most effective methods for increasing metabolite syntheses. Use of endophytes as plant growth promotors could help to ensure continuous supply of medicinal plants, and mitigate issues with fear of extinction. Endophytes minimize heavy metal toxicity in medicinal plants. It has been hypothesized that when medicinal plants are exposed to harsh conditions, associated endophytes are the primary signalling channels that induce defensive reactions. Endophytes go through different biochemical processes which lead to activation of defence mechanisms in the host plants. Thus, through signal transduction pathways, endophytic microorganisms influence genes involved in the generation of secondary metabolites by plant cells. Additionally, elucidating the role of gene clusters in production of secondary metabolites could expose factors associated with low secondary metabolites by medicinal plants. Promising endophyte strains can be manipulated for enhanced production of metabolites, hence, better probability of novel bioactive metabolites through strain improvement, mutagenesis, co-cultivation, and media adjustment.

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Section: Endophytes Intervention Towards Improving Limitations Associ...mentioning

confidence: 99%

Contribution of endophytes towards improving plant bioactive metabolites: a rescue option against red-taping of medicinal plants

Tsipinana,

Husseiny,

Alayande

et al. 2023

Front. Plant Sci.

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“…The plant-endophytic fungi interaction is a mutual association helping plants to cope with both biotic and abiotic stresses [31,42,84,85], promoting plant growth by assimilating essential nutrients (potassium, nitrogen and phosphorus) and producing ammonia, siderophores, hormones (auxins, gibberellins and cytokinins) and enzymes [12,13,17,86]. Particularly, secondary metabolites produced by beneficial fungi play a very interesting role in protecting and ameliorating the quality and yield of agricultural crops [40,87].…”

Section: Agricultural Applicationsmentioning

confidence: 99%

“…Natural source contamination is mainly caused by uncontrolled industrial discharges and anthropogenic activities [17], leading to the accumulation of recalcitrant pollutants, heavy metals, herbicides, pesticides, chlorinated products, etc. [120,121].…”

Section: Bioremediation Applicationsmentioning

confidence: 99%

“…Endophytes are conventionally known as microbes existing in all plant endospheric tissues (roots, shoots, fruits, leaves, flowers, seeds, etc.) without causing Forests 2021, 12, 1784 2 of 13 harmful consequences to the host plant [14][15][16][17]. These microorganisms are usually more abundant in roots and they can be transferred horizontally and vertically [18].…”

Section: Introductionmentioning

confidence: 99%

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Potentials of Endophytic Fungi in the Biosynthesis of Versatile Secondary Metabolites and Enzymes

Slama¹,

Bouket

Alenezi

et al. 2021

Forests

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World population growth and modernization have engendered multiple environmental problems: the propagation of humans and crop diseases and the development of multi-drug-resistant fungi, bacteria and viruses. Thus, a considerable shift towards eco-friendly products has been seen in medicine, pharmacy, agriculture and several other vital sectors. Nowadays, studies on endophytic fungi and their biotechnological potentials are in high demand due to their substantial, cost-effective and eco-friendly contributions in the discovery of an array of secondary metabolites. For this review, we provide a brief overview of plant–endophytic fungi interactions and we also state the history of the discovery of the untapped potentialities of fungal secondary metabolites. Then, we highlight the huge importance of the discovered metabolites and their versatile applications in several vital fields including medicine, pharmacy, agriculture, industry and bioremediation. We then focus on the challenges and on the possible methods and techniques that can be used to help in the discovery of novel secondary metabolites. The latter range from endophytic selection and culture media optimization to more in-depth strategies such as omics, ribosome engineering and epigenetic remodeling.

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“…The use of microorganisms is one of the most promising biological control methods to suppress plant diseases. Novel approaches involve the use of plant growth promoting bacteria (PGPB) which are categorized as those associated with the surface of plant roots, called rhizobacteria, and those that live inside plant tissues, called endophytes [23,24]. These bacteria are known for their abilities to promote and stimulate plant growth and protect host plants against phytopathogens [25].…”

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confidence: 99%

Critical Evaluation of Biocontrol Ability of Bayoud Infected Date Palm Phyllospheric Bacillus spp. Suggests That In Vitro Selection Does Not Guarantee Success in Planta

et al. 2022

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The vascular wilt of date palm (Phoenix dactylifera L.), also known as Bayoud and caused by Fusarium oxysporum f.sp. albedinis (FOA), is the most destructive disease in North Africa. The disease has resulted in huge economic losses due to declining crop yield and quality. The use of potential biocontrol agents is a sustainable and environmentally friendly strategy compared to synthetic fungicides. The use of date palm-associated microflora for the screening of native antagonistic bacteria with potential applications is the most promising way to control this disease. Thus, the epidemic palm groves (in the valley of M’zab-Ghardaia-Algeria) were chosen for the isolation of rhizospheric bacteria and endophytes from the soil and roots of healthy or infected plants. A total of 8 bacterial isolates (83, 84, 300, 333, 322, 260, 249, and 227) selected from 75 FOA-active strains, showed strong activity against several strains of FOA and other major plant pathogens. Their phylogenetic identification proved they belong to the genus Bacillus (Bacillus sp., B. subtilis, B. atrophaeus, B. halotolerans, B. amyloliquefasiens, and B. paralicheformis). Bacterial cultures and a cell-free culture supernatant tested alone or in co-culture showed FOA inhibitory activity. However, the effect of the co-culture did not show any synergy in both cases. Unlike diffusible compounds, volatile organic compounds did not show a significant antifungal ability. The concept of selecting biocontrol agents in vitro does not always guarantee in vivo performance. In addition to antibiosis, other strategies such as competition and resistance induction are required for biocontrol agent efficacy. To evaluate the biocontrol effect in vivo, germinated seeds of date palm were treated with bacteria, infected with the pathogen, and then incubated for 45 days at room temperature in the dark. The majority of the strains (84, 300, and 333) tested showed moderate fungistatic effects and the protection rate reached an average of 60%. In particular, promising results were obtained with B. paralicheniformis strain 260 which significantly protected palm seeds up to 85%, compared to its in vitro test where a low inhibition rate (27.77%) was recorded. Screening methods should be equipped with multifactorial or plant-mediated control mechanisms. Furthermore, these bacteria have shown other potentialities to improve plant growth and resist stressful conditions. Thus, an effective biocontrol agent must combine several beneficial characteristics to be used successfully in situ. In this respect, Bacillus remains the best candidate for biological control.

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Biotechnology and Bioinformatics of Endophytes in Biocontrol, Bioremediation, and Plant Growth Promotion

Cited by 7 publications

References 156 publications

Contribution of endophytes towards improving plant bioactive metabolites: a rescue option against red-taping of medicinal plants

Contribution of endophytes towards improving plant bioactive metabolites: a rescue option against red-taping of medicinal plants

Potentials of Endophytic Fungi in the Biosynthesis of Versatile Secondary Metabolites and Enzymes

Critical Evaluation of Biocontrol Ability of Bayoud Infected Date Palm Phyllospheric Bacillus spp. Suggests That In Vitro Selection Does Not Guarantee Success in Planta

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