Biotechnological valorisation of raw glycerol discharged after bio-diesel (fatty acid methyl esters) manufacturing process: Production of 1,3-propanediol, citric acid and single cell oil

Papanikolaou, Séraphim; Fakas, Stylianos; Fick, Michel; Chevalot, Isabelle; Galiotou‐Panayotou, Maria; Komaitis, Michael; Marc, Ivan; Aggelis, George

doi:10.1016/j.biombioe.2007.06.007

Cited by 357 publications

(298 citation statements)

References 42 publications

Supporting

Mentioning

243

Contrasting

Unclassified

Order By: Relevance

“…On the basis of microorganisms grown on biodiesel-derived waste glycerol, researchers developed the method of production of 1,3-propanediol [16], biomass-enriched lipids [17][18][19][20][21], carbonic acids (in particular, pyruvic acid [22]), α-ketoglutaric acid [23,24], citric acid [16,25,26], and butanol [27]. However, few reports showed the satisfactory growth of fungi of the genera Mucor, Cunninghamella, Thamnidium, Zygorhynchus, and Mortierella, and similarly for lipid synthesis when cultivated on glycerol [15][16][17][18][19]21,[28][29][30][31][32][33]. We have previously shown that both pure glycerol and glycerol-containing wastes of biodiesel production can be successfully used for AA synthesis by selected M. alpina strains [20,29].…”

Section: Introductionmentioning

confidence: 99%

The Effect of pH and Temperature on Arachidonic Acid Production by Glycerol-Grown Mortierella alpina NRRL-A-10995

et al. 2018

View full text Add to dashboard Cite

Arachidonic acid (AA) has a wide range of applications in medicine, pharmacology, diet, infant nutrition, and agriculture, due to its unique biological properties. The microbiological processes involved in AA production usually require carbohydrate substrates. In this paper, we propose a method for AA production from glycerol, an inexpensive and renewable carbon substrate that is produced by the fungal strain, Mortierella alpina NRRL-A-10995. Our experimental results showed that the optimum pH values required for fungal growth and the production of lipids and AA were different and depended on the growth phase of the fungus. The AA production was shown to be extremely sensitive to acidic pH values and was completely inhibited at a pH of 3.0. The optimum temperature for AA production was 20-22 • C. Continuous cultivation of M. alpina occurred in a glycerol-containing medium, and growth limitations were implemented through the addition of nitrogen and the selection of optimal conditions (pH 6.0, 20 • C). This ensured that active AA production occurred (25.2% of lipids and 3.1% of biomass), with the product yield from the consumed glycerol being 1.6% by mass and 3.4% by energy.

show abstract

Section: Introductionmentioning

confidence: 99%

The Effect of pH and Temperature on Arachidonic Acid Production by Glycerol-Grown Mortierella alpina NRRL-A-10995

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The initial and remaining sugar levels were measured by dinitrosalicylic acid (DNS) method while the level of citric acid was measured using acetic anhydride pyridine method (18,19).…”

Section: Determining Methodsmentioning

confidence: 99%

Citric Acid Production from Sugarcane Bagasse through Solid State Fermentation Method Using Aspergillus niger Mold and Optimization of Citric Acid Production by Taguchi Method

Yadegary

Hamidi

Alavi

et al. 2013

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Citric acid is produced in insignificant quantities in Iran. Despite its great range of utilizations, and from another aspect, high level of production of sugarcane bagasse, the related problems arising from maintenance of this acid require thinking of a measure in the direction of its optimal usage and production. Objectives: The objective of the present study is to obtain effectual variables in producing citric acid from sugarcane bagasse through Solid State Fermentation (SSF) method using Aspergillus niger mold and to optimize its mass production by employing Taguchi method. Materials and Methods:The effective parameters such as spore inoculation level, methanol percentage, solvent type, spore age, humidity percentage, initial pH of substrate, fermentation period and temperature, initial sugar percentage, autoclaving duration, nitrogen source and etc. were studied for producing citric acid from sugarcane bagasse with respect to Tagouchi method. Results: By considering the findings obtained from the tests, the highest production rate of citric acid g/kg out of untreated sugarcane bagasse is 75.45 based on the consumed sugar and a yield of 15.1 g/kg of sugarcane was achieved per day. Application of sodium hydroxide and acid pretreated sugarcane bagasse increased the production of citric acid in such a fashion that the production rates were 97.81 g/kg and 87.32 g/kg of sugarcane bagasse, respectively, compared to sodium hydroxide and acid untreated sugarcane bagasse. Conclusions:The obtained findings in the present study indicated that sugarcane bagasse is an ideal substrate in producing citric acid and the aforementioned process could be considered as a beneficial and cost-effective method in citric acid production.

show abstract

“…It also resulted in the production of citric acid and single-cell oil in a single reaction [21,111]. Acetate-negative mutant strain of Y. lipolytica Wratislavia AWG7 fed on CG lead to citric acid yield of 131.5 g/L, which was as effective as that produced on pure glycerol (139 g/L).…”

Section: Other Bioproductsmentioning

confidence: 99%

“…Clostridium butyricum was shown to produce 1,3-PDO from synthetic medium supplemented with CG [19][20][21][22]. C. butyricum strain F2b could convert CG into 1,3-PDO under batch and continuous culture conditions, with yields of 44-47 g/dry matter.…”

Section: Propanediolsmentioning

confidence: 99%

Biorefinery for Glycerol Rich Biodiesel Industry Waste

2016

View full text Add to dashboard Cite

The biodiesel industry has the potential to meet the fuel requirements in the future. A few inherent lacunae of this bioprocess are the effluent, which is 10 % of the actual product, and the fact that it is 85 % glycerol along with a few impurities. Biological treatments of wastes have been known as a dependable and economical direction of overseeing them and bring some value added products as well. A novel eco-biotechnological strategy employs metabolically diverse bacteria, which ensures higher reproducibility and economics. In this article, we have opined, which organisms and what bioproducts should be the focus, while exploiting glycerol as feed.

show abstract

Biotechnological valorisation of raw glycerol discharged after bio-diesel (fatty acid methyl esters) manufacturing process: Production of 1,3-propanediol, citric acid and single cell oil

Cited by 357 publications

References 42 publications

The Effect of pH and Temperature on Arachidonic Acid Production by Glycerol-Grown Mortierella alpina NRRL-A-10995

The Effect of pH and Temperature on Arachidonic Acid Production by Glycerol-Grown Mortierella alpina NRRL-A-10995

Citric Acid Production from Sugarcane Bagasse through Solid State Fermentation Method Using Aspergillus niger Mold and Optimization of Citric Acid Production by Taguchi Method

Biorefinery for Glycerol Rich Biodiesel Industry Waste

Contact Info

Product

Resources

About