Biotechnological production of value-added carotenoids from microalgae

Wichuk, Kristine; Brynjólfsson, Sigurður; Fu, Weiqi

doi:10.4161/bioe.28720

Cited by 63 publications

(44 citation statements)

References 31 publications

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“…Nutritional supply, D. salina, may be used as an alternative to hormonal treatments to enhance reproductive performance. D. salina has high levels of beta-carotene, glycerol, protein and other fine chemicals (Gouveia et al, 2008;Ghasemi et al, 2011;Wichuk et al, 2014;Cuellar-Bermudez et al, 2015;Gong and Bassi, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…D. salina has high levels of beta-carotene, glycerol, protein and other fine chemicals (Gouveia et al, 2008;Ghasemi et al, 2011;Wichuk et al, 2014;Cuellar-Bermudez et al, 2015;Gong and Bassi;, which might improve the quality of oocyte and support further embryonic development. Conclusion of our work (Senosy et al, 2017) indicated that D. salina supplemented to Boer goats (10 g/day/head) accelerated ovarian follicle development and significantly, increased follicle numbers (small, medium and large follicles).The improvement of oocyte quality in D. salina group compared to control one might be related to high levels of beta-carotene and elevation of plasma metabolites and hormones levels.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

“…Because of the negative impacts of increased protein and energy in the diet on health and fertility (McEvoy et al, 1997;Dawuda et al, 2002) and the high cost of ration, it is important to search for non-traditional supplements with beneficial character-istics for body. Microalgae possesses high concentration of proteins, lipids and vitamins, sulfated polysaccharides, several amounts of polyunsaturated fatty acids (Gouveia et al, 2008;Ghasemi et al, 2011;Wichuk et al, 2014;Cuellar-Bermudez et al, 2015;Gong and Bassi;. Pulz and Gross (2004) showed that polysaccharides is important as therapeutic where it support immune system and has potential role to fight virus infection (Abdel-Daim et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Development of Oocytes and Preimplantation Embryos of Mice Fed Diet Supplemented with Dunaliella salina

Mohammed¹

2017

Adv. Anim. Vet. Sci.

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| The present study investigated the developmental competence of germinal vesicle (GV) oocytes and the resulting embryos upon dietary Dunaliella salina (DS) supplementation. Quality of GV oocytes and maturation, timing of zygotes cleavage to two-cell stage, quality of embryos and offspring number and weight were investigated of mice supplemented with DS (100g/kg ration; N=30) compared to control (N=30).Females were injected with 7.5 I.U. of equine chorionic gonadotropin (PMSG) followed by 7.5 I.U. of human chorionic gonadotropin (hCG) after 48h and mated with fertile male. Immature GV oocytes were harvested from ovaries after 48h of PMSG injection for investigating quality of oocyte, timing of breakdown of germinal vesicle (GVBD) and extrusion of first polar bodies and maturation rate (%).Zygotes were harvested from uterine tubes 29-30hafterhCG injection and followed for first cleavage whereas quality of blastocysts were evaluated after collection from uterine horn at96h of hCG. Improvement of oocyte quality has been indicated in DS group whereas GVBD and maturation rate were not differed between DS and control groups. Although D. salina did not change timing of first cleavage of zygotes, blastocysts' quality were significantly (P<0.05) increased in DS group. Furthermore, offspring number (9.9 ± 0.26 & 8.2 ± 0.30) and weight (12.01 ± 0.31 & 9.84 ± 0.37) at birth were increased significantly (P<0.05) in the DS group compared to control one. In conclusion, supplementation of D. salina could increase reproductive performance through improvement quality of germinal vesicle oocytes and preimplantation embryos.

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Section: Discussionmentioning

confidence: 99%

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of Oocytes and Preimplantation Embryos of Mice Fed Diet Supplemented with Dunaliella salina

Mohammed¹

2017

Adv. Anim. Vet. Sci.

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“…The highest β‐carotene content has been recorded in the halotolerant algae, Dunaliella bardawil (Chlorophyceae), which produced about 80 mg g −1 cell originally but the biomass content was not improved . Except for D. bardawil , the cellular content of β‐carotene in recombinant C. zofingiensis and Saccharomyces cerevisiae cells was less than 6 mg g −1 cell, and higher quantities are needed to simplify the downstream processing and improve the economics of the production processes. The production of β‐carotene with recombinant S. cerevisiae was not achieved by overexpressing PSY because carotene production in yeasts is through crt cluster genes (instead of psy gene) in carotenogenic pathway .…”

Section: Introductionmentioning

confidence: 99%

Expression of Synthetic Phytoene Synthase Gene to Enhance β‐Carotene Production in Scenedesmus sp. CPC2

Chen

Kao²,

Tsai³

et al. 2017

Biotechnology Journal

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β-carotene is a valuable pigment abundant in some microalgal species but the low β-carotene productivity of microalgae has become the major obstacles against its commercialization. This work aims to improve the productivity of algae-based β-carotene via genetic engineering approaches. First, a synthetic psy gene construct (891 bp) encoding 297 amino acids is expressed in Scenedesmus sp. CPC2 host to enhance the β-carotene production. The synthetic psy gene is designed by considering the highest consensus of amino acids (i.e., 62% identity) from Chlamydomonas reinhardtii, Dunaliella salina, and Mariella zofingiensis. The original β-carotene content in wild-type Scenedesmus sp. CPC2 is 10.8 mg g -cell when grown on BG11 medium under 2% CO aeration, 150 μmol m s light intensity and 25°C. After transformation of the psy gene into the microalgal host, the β-carotene content of the best recombinant strain (i.e., transformant CPC2-4) significantly increased to over 30 mg g -cell. The optimal production of β-carotene with the CPC2-4 recombinant strain was achieved when the strain is grown on BG11 medium amended with 0.075 g of MgSO , giving approximately 3-fold higher β-carotene content than that of the wild-type strain. The best cellular β-carotene content obtained (i.e., 31.8 mg g ) is superior to most algae-based β-carotene production performance reported in the literature.

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“…Mutagenesis, adaptive laboratory evolution (or ALE), and genetic engineering have been established as strategies for developing algal cell factories. Furthermore, systems biology and synthetic biology perspectives and approaches are emerging in the production of bioactive compounds, such as carotenoids and vitamins, in algae [6,7]. In addition, using in silico metabolic models to complement and contextualize genetic and metabolic data will provide researchers more options for the strain improvement of target products [8,9,10,11].…”

Section: Introductionmentioning

confidence: 99%

Algal Cell Factories: Approaches, Applications, and Potentials

Fu¹,

Chaiboonchoe²,

Khraiwesh

et al. 2016

Marine Drugs

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With the advent of modern biotechnology, microorganisms from diverse lineages have been used to produce bio-based feedstocks and bioactive compounds. Many of these compounds are currently commodities of interest, in a variety of markets and their utility warrants investigation into improving their production through strain development. In this review, we address the issue of strain improvement in a group of organisms with strong potential to be productive “cell factories”: the photosynthetic microalgae. Microalgae are a diverse group of phytoplankton, involving polyphyletic lineage such as green algae and diatoms that are commonly used in the industry. The photosynthetic microalgae have been under intense investigation recently for their ability to produce commercial compounds using only light, CO2, and basic nutrients. However, their strain improvement is still a relatively recent area of work that is under development. Importantly, it is only through appropriate engineering methods that we may see the full biotechnological potential of microalgae come to fruition. Thus, in this review, we address past and present endeavors towards the aim of creating productive algal cell factories and describe possible advantageous future directions for the field.

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Biotechnological production of value-added carotenoids from microalgae

Cited by 63 publications

References 31 publications

Development of Oocytes and Preimplantation Embryos of Mice Fed Diet Supplemented with Dunaliella salina

Development of Oocytes and Preimplantation Embryos of Mice Fed Diet Supplemented with Dunaliella salina

Expression of Synthetic Phytoene Synthase Gene to Enhance β‐Carotene Production in Scenedesmus sp. CPC2

Algal Cell Factories: Approaches, Applications, and Potentials

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