Expression of Synthetic Phytoene Synthase Gene to Enhance β‐Carotene Production in <i>Scenedesmus</i> sp. CPC2

Chen, Chun‐Yen; Kao, Ai-Ling; Tsai, Zheng-Chia; Shen, Yu‐Mei; Kao, Pei-Hsun; Ng, I‐Son; Chang, Jo‐Shu

doi:10.1002/biot.201700204

Cited by 51 publications

(15 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The absorbed energy can be transferred to chlorophylls by primary carotenoid like lutein, thus expanding the light-absorbing spectrum of microalgae [ 6 ]. Moreover, due to their anti-oxidant properties, carotenoids play an important role in alleviating certain cancers, premature aging, cardiovascular diseases, and arthritis [ 7 , 8 ]. Furthermore, carotenoids are also often used as coloring agents and dyes because of their intrinsic color [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Sun

Ren

Zhao

et al. 2018

Biotechnol Biofuels

318

129

View full text Add to dashboard Cite

Microalgae have drawn great attention as promising sustainable source of lipids and carotenoids. Their lipid and carotenoids accumulation machinery can be trigged by the stress conditions such as nutrient limitation or exposure to the damaging physical factors. However, stressful conditions often adversely affect microalgal growth and cause oxidative damage to the cells, which can eventually reduce the yield of the desired products. To overcome these limitations, two-stage cultivation strategies and supplementation of growth-promoting agents have traditionally been utilized, but developing new highly adapted strains is theoretically the simplest strategy. In addition to genetic engineering, adaptive laboratory evolution (ALE) is frequently used to develop beneficial phenotypes in industrial microorganisms during long-term selection under specific stress conditions. In recent years, many studies have gradually introduced ALE as a powerful tool to improve the biological properties of microalgae, especially for improving the production of lipid and carotenoids. In this review, strategies for the manipulation of stress in microalgal lipids and carotenoids production are summarized and discussed. Furthermore, this review summarizes the overall state of ALE technology, including available selection pressures, methods, and their applications in microalgae for the improved production of lipids and carotenoids.

show abstract

Section: Introductionmentioning

confidence: 99%

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Sun

Ren

Zhao

et al. 2018

Biotechnol Biofuels

318

129

View full text Add to dashboard Cite

show abstract

“…On the other hand, Scenedesmus can produce important pigments carotenoids as valuable products, and overexpression of synthetic phytoene synthase (psy) gene in Scenedesmus sp. CPC2 increased β-carotene production to 30 mg g −1 -cell [34]. So far no studies have been focused on the innate carotenoid biosynthetic genes in S. obliquus.…”

Section: Discussionmentioning

confidence: 99%

Genome sequencing, assembly, and annotation of the self-flocculating microalga Scenedesmus obliquus AS-6-11

Chen

Mhuantong²,

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background Scenedesmus obliquus belongs to green microalgae and is widely used in aquaculture as feed, which is also explored for lipid production and bioremediation. However, genomic studies of this microalga have been very limited. Cell self-flocculation of microalgal cells can be used as a simple and economic method for harvesting biomass, and it is of great importance to perform genome-scale studies for the self-flocculating S. obliquus strains to promote their biotechnological applications. Results We sequenced the genome of the self-flocculating microalga S. obliquus AS-6-11 employing the Pacific Biosciences sequencing platform, and used the MECAT software for de novo genome assembly. The estimated genome size of S. obliquus AS-6-11 is 172.3 Mbp with an N50 of 94,410 bp, and 31,964 protein-coding genes were identified. Gene Ontology (GO) and KEGG pathway analysis revealed 65 GO terms and 428 biosynthetic pathways. Comparing to the well-studied green microalgae Chlamydomonas reinhardtii, Chlorella variabilis, Volvox carteri and Micractinium conductrix, the genome of S. obliquus AS-6-11 encodes more unique proteins, and some of them might be responsible for the self-flocculating phenotype. These genes encode D-mannose binding lectin, glycosylphosphatidylinositol (GPI)-anchored cell wall proteins, and proteins with fasciclin domains that are commonly found in cell surface proteins, respectively. Four genes encoding both GPI-anchored cell wall proteins and fasciclin domain proteins are the most interesting targets for further studies. Conclusions To our best knowledge, this is the first report on the in-depth annotation of the S. obliquus genome, and the results will facilitate functional genomic studies of this important microalga. The comparative genomic analysis here also provides new insights into the evolution of green microalgae. Furthermore, identification of the potential genes encoding proteins for the self-flocculation of microalgal cells will benefit studies on the molecular mechanism underlying this phenotype for its better control and biotechnological applications as well.

show abstract

“…The over-expression of Chlorella psy enhances the accumulation of violaxanthin and lutein in Chlamydomonas and of β-carotene in Scenedesmus sp. [ 11 , 15 ]. The heterologous expression of the lycopene β-cyclase/lycopene ε-cyclase/light-harvesting complex-fusion protein from the green alga Ostreococcus lucimarinus allowed the modulation of the ratio between α- and β-pathways in bacteria.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Culture Conditions for the Optimization of Carotenoid Production in Several Strains of the Picoeukaryote Ostreococcus

et al. 2018

View full text Add to dashboard Cite

Microalgae are promising sources for the sustainable production of compounds of interest for biotechnologies. Compared to higher plants, microalgae have a faster growth rate and can be grown in industrial photobioreactors. The microalgae biomass contains specific metabolites of high added value for biotechnology such as lipids, polysaccharides or carotenoid pigments. Studying carotenogenesis is important for deciphering the mechanisms of adaptation to stress tolerance as well as for biotechnological production. In recent years, the picoeukaryote Ostreococcus tauri has emerged as a model organism thanks to the development of powerful genetic tools. Several strains of Ostreococcus isolated from different environments have been characterized with respect to light response or iron requirement. We have compared the carotenoid contents and growth rates of strains of Ostreococcus (OTTH595, RCC802 and RCC809) under a wide range of light, salinity and temperature conditions. Carotenoid profiles and productivities varied in a strain-specific and stress-dependent manner. Our results also illustrate that phylogenetically related microalgal strains originating from different ecological niches present specific interests for the production of specific molecules under controlled culture conditions.

show abstract

Expression of Synthetic Phytoene Synthase Gene to Enhance β‐Carotene Production in Scenedesmus sp. CPC2

Cited by 51 publications

References 31 publications

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Genome sequencing, assembly, and annotation of the self-flocculating microalga Scenedesmus obliquus AS-6-11

Comparative Analysis of Culture Conditions for the Optimization of Carotenoid Production in Several Strains of the Picoeukaryote Ostreococcus

Contact Info

Product

Resources

About