Biotechnological production of centellosides in cell cultures of Centella asiatica (L) Urban

Abstract

Centella asiatica (L.) Urban plants have been used since ancient times for their medicinal properties, and their extracts have proven antioxidant, wound healing, sedative, and neuroprotective activities, among others. The natural compounds responsible for C. asiatica bioactivity are triterpene saponins formed from the dammarene branch of the triterpene biosynthetic pathway, collectively known as centellosides, with madecassoside and asiaticoside and their aglycones, madecassic acid and asiatic acid being the most important. Several biotechnological approaches have been developed for the bioproduction of centellosides, based on cell, hairy root, and in vitro plant cultures. This review summarizes the main therapeutic properties of these compounds, as well as their biosynthetic pathways, referring to genetic studies that have identified genes involved in their formation. The biotechnological production of centellosides from a small scale to bioreactor level is also covered. Finally, we summarize the most effective strategies for increasing centelloside yield, including recent transcriptomic, proteomic, and metabolomic studies that have gained new insights into the centelloside biosynthetic pathway and its control.

An optimized biotechnological system for the production of centellosides based on elicitation and bioconversion of Centella asiatica cell cultures

“…Centella asiatica cell line UBCA17 is characterized by a high capacity for biomass and centelloside production under elicitation [15]. The positive effects of treatments with elicitors such as MeJA, SA, copper ions, and YE on centelloside production have been previously demonstrated in both C. asiatica plant cell and hairy root cultures [7]. In this work, we corroborated the positive effect of MeJA, SA, and YE on centelloside biosynthesis, and also for the first time demonstrated the effectiveness of the new elicitor CORO in increasing centelloside production (more than fivefold), even at concentrations 100-fold lower than MeJA, without affecting the growth capacity of the cell cultures ( Fig.…”

Production of Camptothecin from Nothapodytes nimmoniana: An Overview

“…Therefore, biotechnological production methods constitute an alternate and efficient way for obtaining specific secondary metabolites. The abatement in the diversity of plant by uncommon cutting and exploitation lead to various tissue culture methods to preserve the plant diversity and production of concerned products from it [33] .…”

Mass Propagation of Plant Cells – An Emerging Technology Platform for Sustainable Production of Biopharmaceuticals

“…PCCT is a powerful technology platform for production of bioactive phytochemicals, active cosmetic additives, food supplements and nutraceuticals, which in general utilizes non-genetically modified plant cells, grown under controlled microenvironment in a form of suspended single cells and/or small aggregates. Several pharmaceutically active compounds, including: tropane alkaloids atropine, hyoscyamine and scopolamine [4]; benzylisoquinoline alkaloid protopine [5]; diterpenoids as paclitaxel [6]; triterpenoids as ursolic and oieanolic acids [7,8]; pentacyclic triterpenoid saponins as centellosides [9]; stilbenoids as resveratrol [10]; aryl tretralin lactone podophyllotoxin [11]; phenylethanoid glycosides as verbascoside and leucosceptoside A [12]; etc. have been found to accumulate in plant cell suspensions.…”