Biotechnological aspects of Amixin® application as an antiviral drug for treatment of pigs and chicken

Abstract

Veterinary drug AmixinR, active substance dihydrochloride 2,7-bis[2-(diethylamine)ethoxy]fluorene-9-one (AMX), was tested for its antiviral activity with using of the epizootic relevance for Ukraine the infectious agents of Pseudorabies (PR), Teschovirus encephalomyelitis (TEM), classical swine fever (CSF), porcine reproductive and respiratory syndrome pigs (PRRS), 2nd type of porcine circovirus (PCV-2) and parvovirus infections (PPVI), swine (SIV) and avian influenza viruses (AIV). The influence of this drug on agents of the avian mycoplasmosis (Myc) and pasteurellosis (Past) was also learned. AMX action was tested in the concentrations of 0.5–15 mg/ml, expositions for 0.25–12 hours and at the room temperature. Under these conditions, the inactivation of 20–60% (the highest concentrations more likely) the 1000 infectious units (TCID, ELD or PFU50/ml, respectively) of all viruses was resulted. The 5000 ELD50/ml of the SIV and AIV viruses were inactivated for 5 hours almost totally. Moderate toxicity of AMX was registered in it doses ≥1 mg/ml (P≤0.01) for tube cultures of PK-15 cell line and Marc-145. At the same time AMX doses ≥0.125 mg/ml inhibited by 20–75% of infective activities of agents of the PR, PRRS, PCV-2, PPVI (n=42, P≤0.01) in cell cultures Marc-145 and PK-15, respectively. The toxicity of the drug for 7–9-days-old embryos chickens began to emerge with a concentration of 1.5 mg/ml (n=18, P≤0.01). Its virostatic effect on SIV, AIV and PR agents was manifested in doses ≥0.25 mg/ml (n=24, P≤0.01). AMX was administrated in a single dose of 540 mg ana partes with sunflower oil and showed no toxicity for suckling piglets (n=4, P≤0.01) and 10-days-old chickens (n=10, P≤0.005). At the same time the antibiotic resistant Myc and Past from the blood of chicken infected by natural mixes of these agents, acquire the sensitivity to commercial food antibiotic after 5-days course of treatment by AMX. These data is interpreted the mechanism of therapeutic and preventive action of AMX through direct antivirus activity. The hypothesis of acquires the antibiotic sensitivity by pathogenic bacteria throughout its bacteriophages inhibition is proposed.