Biotechnological Aspects and Perspective of Microbial Keratinase Production

Gopinath, Subash C.B.; Anbu, Periasamy; Lakshmipriya, Thangavel; Tang, Thean‐Hock; Chen, Yeng; Hashim, U.; Ruslinda, A. Rahim; Arshad, M. K. Md

doi:10.1155/2015/140726

Cited by 87 publications

(57 citation statements)

References 61 publications

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“…2 Changes in pH of basal salt medium after keratin degradation by Chrysosporium queenslandicum; Data means and error bars at each point indicate ± SE. Statistical analysis showed a significant difference (P \ 0.05) among tested treatments use keratinous substrates as source of carbon and nitrogen (Gopinath et al 2015). Keratinous waste degradation in BSM with microbial cultures is one of the most capable techniques to utilize keratin protein and to obtain important by-products (Laba and Rodziewicz 2014).…”

Section: Discussionmentioning

confidence: 99%

Chrysosporium queenslandicum: a potent keratinophilic fungus for keratinous waste degradation

Kumawat¹,

Sharma²,

Bhadauria³

2017

Int J Recycl Org Waste Agricult

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Purpose Keratinous wastes are the solid environmental pollutant generated from poultry farms, slaughterhouses and barber's shops. The aim of the present study is the degradation of keratinous wastes in an eco-friendly way by biological methods, which should further be helpful to reduce the wastes and recycled into valuable feed and fertilizers. Methods Degradation of keratinous substrates was assessed by highly potent keratinophilic fungi, namely Chrysosporium queenslandicum TKKASb Apinis and R.G. Rees. This chicken feather degrading fungal strain previously isolated and identified by morphological and 18 s rDNA sequencing in laboratory, was used in the present study. Ch. queenslandicum was inoculated into the basal salt medium (BSM) with keratinous substrates for 12 and 24 days at 28 ± 2°C to observe degradation. The rate of degradation was expressed as weight loss of keratin substrate over incubation days and pH variation. Results In this study, Ch. queenslandicum showed maximum degradation on chicken feathers followed by human nail clippings, animal hair and human hair. The degradation rate on chicken feather was 38.40 ± 0.80 and 46.40 ± 2.50% after 12 and 24 days, respectively. The basal salt medium's pH was increased over incubation time. Scanning electron microscopy (SEM) examination also demonstrated the degradation of chicken feathers. Conclusions The results suggest that Ch. queenslandicum possess the potential biotechnological applications which can be used in the hydrolysis of keratinous waste and recycling of poultry waste for environmental protection. The hydrolyzed keratinous material can also be utilized as the source of fertilizers for plants and feed for animals.

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Section: Discussionmentioning

confidence: 99%

Chrysosporium queenslandicum: a potent keratinophilic fungus for keratinous waste degradation

Kumawat¹,

Sharma²,

Bhadauria³

2017

Int J Recycl Org Waste Agricult

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“…), or bacteria (e.g. Bacillus subtilis and Bacillus licheniformis ) (Gopinath et al, 2015;More, 2013;Selvam, Vishnupriya, & Yamuna, 2013). Feather degradation mainly depends on keretinase enzymes which are responsible for releasing peptides from keratin (Nickerson & Durand, 1963) after reduction of native keratine to reduced keratin by disulfite reductase enzyme (Sangali & Brandelli, 2000).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Keratin Degrading Alkaliphiles

Elsayed

Mowafy

Elrazak

et al. 2016

JBLS

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Feather which mainly contains Keratin is one of the main poultry industry wastes and at the same time with high nutritional value if converted to soluble form. In this study, we isolated and characterized four alkaliphilic bacteria for their ability to degrade feather under alkaline conditions. Two of the isolates have been identified as Lysinibacillus spp. and both of them are growing optimally at 30 °C and pH 9. The others were identified as Micrococcus sp. and Nocardiopsis sp. and both of them showed optimum growth at 37 °C and pH 10.5. All the isolates are promising and potential tools that could be used for keratin degradation.

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“…The feather waste contains a number of keratin proteins that have very low digestibility (Gopinath et al, 2015). Keratin is a biopolymer that structures the feather waste (Martínez-Hernández and Velasco-Santos, 2012;Amieva et al, 2014;Cao et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Feather Protein Concentrates Hydrolyzed Using Bacillus subtilis FNCC 0059

Said¹,

Abustam²,

Yuliati³

et al. 2018

OnLine Journal of Biological Sciences

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Fermentation technology is one technology that can be applied in the process of hydrolysis and decomposition of keratin protein in chicken feather waste. The fermentation technology can use microbial bacteria as a decomposer to improve the digestibility of the Feather Waste (FW). The purpose of this study was to evaluate the properties of chicken feather waste and Feather Protein Concentrate (FPC) fermented using Bacillus subtilis FNCC 0059. The properties of the feather-waste as control and FPC were studied specifically related to the dry matter protein digestibility properties performed in-vitro, water content, protein content, fat content, fiber content, NDF levels and amino acid profile. A total of 2 types of samples are compared the properties, namely FPC and FW. Bacterial isolates of Bacillus subtilis FNCC 0059 enriched with nutrient broth and propagated in a mixture of molasses and nutrient broth solution as a fermentation agent. The fermentation process was carried out for 4 weeks in the incubator at 35 °C. The data were processed statistically by using Ttest to compare the properties of FW with FPC fermentation for 4 weeks. The results showed that the properties of In-vitro Protein Digestibility (IvPD), moisture content, fiber content, NDF significantly differed (p>0.05) between FW and FPC, while protein and fat content were not significantly different (p>0.05). The amino acid profile in both samples has similarities.

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Biotechnological Aspects and Perspective of Microbial Keratinase Production

Cited by 87 publications

References 61 publications

Chrysosporium queenslandicum: a potent keratinophilic fungus for keratinous waste degradation

Chrysosporium queenslandicum: a potent keratinophilic fungus for keratinous waste degradation

Characterization of Keratin Degrading Alkaliphiles

Characteristics of Feather Protein Concentrates Hydrolyzed Using Bacillus subtilis FNCC 0059

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