Biosynthetic pathways for 3-hydroxypropionic acid production

Abstract

Biobased platform chemicals have attracted growing interest recently. Among them, 3-hydroxypropionic acid receives significant attention due to its applications in the synthesis of novel polymer materials and other derivatives. To establish a biotechnology route instead of the problematic chemical synthesis of 3-hydroxypropionic acid, biosynthetic pathway is required, and the strategies of how to engineer a microbe to produce this product should be considered. In the present review, we summarize and review all known pathways, which could be potentially constructed for 3-hydroxypropionic acid production. Mass and redox balances are discussed in detail. Thermodynamic favorability is evaluated by standard Gibbs free energy. The assembly of pathways and possible solutions are proposed. Several new techniques and future research needs are also covered.

Gene Arrangements in Expression Vector Affect 3-Hydroxypropionic Acid Production in Klebsiella pneumoniae

“…Recently, microbial fermentation instead of chemical synthesis is widely used to generate chemicals. 3-Hydroxypropionic acid (3-HP) ranks the third among the 12 top-value platform chemicals proposed by United States Department of Energy [11,21]. 3-HP can be readily converted into a panel of economically important compounds, such as 1,3-propanediol (1,3-PDO), acrylic acid, and acrylamide [11,18].…”

“…3-Hydroxypropionic acid (3-HP) ranks the third among the 12 top-value platform chemicals proposed by United States Department of Energy [11,21]. 3-HP can be readily converted into a panel of economically important compounds, such as 1,3-propanediol (1,3-PDO), acrylic acid, and acrylamide [11,18]. In addition, 3-HP is a potential nematicide [16], or the monomer of poly-3-hydroxypropionic acid that has advantages over poly-bhydroxybutyrate (PHB) in several physical properties such as tensile strength and elongation at break [1,2].…”

Catalytic routes towards acrylic acid, adipic acid and ε-caprolactam starting from biorenewables

“…Many fermentation routes can produce this compound (eqn (21)). 88 Current yields from glucose are too low for industrial application at high concentrations; although coupled fermentation with co-reactions may overcome this problem. 89 The biobased production of 3-HP is currently not commercialized.…”

Advances in Metabolic Engineering of Saccharomyces cerevisiae for the Production of Industrially and Clinically Important Chemicals

“…3-HP derivatives have a variety of applications in super absorbent polymers, surface coatings, adhesives and paints [11]. Although there are biological pathways to 3-HP via either glycerol, lactate, malonyl-CoA or β-alanine intermediates, no organism is known to produce it as an end product [22]. The pathways based on malonyl-CoA and β-alanine have been constructed in S. cerevisiae [23,24].…”